Identification and DNA typing of two Cw7 alleles (Cw∗0702 and Cw∗0704) in Japanese, with the corrected sequence of Cw∗0702

Wang, Huiru; Tokunaga, Katsushi; Ishikawa, Yoshihide; Asahina, Akihiko; Kuwata, Shoji; Akaza, Tatsuya; Tadokoro, Kenji; Shibata, Yoichi; Takiguchi, Masafumi; Juji, Takeo

doi:10.1016/0198-8859(95)00150-6

Cited by 15 publications

(5 citation statements)

References 15 publications

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“…As previously reported by Wang et al. (23), HLA‐C*0701 and C*0702 differed by two nucleotide substitutions at −7 and −20. All HLA‐A and C alleles examined showed a C‐C sequence at +9 and +10 after the stop codon in the 3′ UTR in comparison to the consensus sequence, whereas the corresponding sequences reported by Summers et al.…”

Section: Resultssupporting

confidence: 82%

“…The HLA‐A and ‐C stop codon was used as a reference point for numbering all 3′‐UTR sequences. The majority of the 5′‐ and 3′‐ UTR sequences adjacent to exon 1 and 7 were identical to those reported previously (19, 23) however, there were some exceptions. We did not detect any substitutions at the start codon of HLA‐A*0301, but a G to C substitution was found at the −7 position of HLA‐A*1101.…”

Section: Resultssupporting

confidence: 82%

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Efficient cloning and expression of HLAclass I cDNA in human B‐lymphoblastoidcell lines

et al. 2002

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Analysis of HLA restriction specificity is one of the important steps in characterizing T cell clones. This usually requires either a panel of HLA-typed cells or HLA cDNA transfectants. Although preparation of HLA cDNA transfectants is laborious, utilization of transfectants is advantageous when a suitable panel is not available due to linkage disequilibrium or rarity of the HLA allele of interest. In this report, we describe an efficient and rapid HLA cloning and expression system. Three sets of PCR primers specific for HLA-A, B and C loci were designed by extensively sequencing 5'- and 3'- untranslated regions of HLA class I genes. The PCR-amplified products were introduced into modified Phoenix retrovirus vectors containing a puromycin resistant gene under the control of a LTR promotor. Gibbon ape leukemia virus (GALV)-pseudotyped retrovirus was produced and infected into B-lymphoid cell lines. Following expansion in selection media, more than 80% of cells expressed transduced HLA at a comparable level to that normally expressed. These results indicate that locus-specific PCR cloning and utilization of GALV-pseudotyped retroviral vector can be an effective and relatively efficient tool for constructing a panel of different HLA transfectants.

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Section: Resultssupporting

confidence: 82%

Section: Resultssupporting

confidence: 82%

Efficient cloning and expression of HLAclass I cDNA in human B‐lymphoblastoidcell lines

et al. 2002

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“…These studies were performed with serology,1, 6 which is an unreliable technique for accurately determining HLA‐C alleles. The progressive development of molecular biology techniques has allowed more accurate detection of histocompatibility alleles 8, 9, 22–24. In malignant thyroid pathology, the only association between the HLA‐Cw7 allele and papillary and follicular carcinomas was found by Ozaki et al18 Our results indicate an association between the lower frequency of the HLA‐Cw7 allele and the presence of differentiated carcinoma.…”

Section: Discussionmentioning

confidence: 48%

Frequency of HLA‐C alleles in differentiated thyroid carcinoma in southeastern Spain

Rı́os

Rodrı́guez

Moya

et al. 2004

Cancer

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BACKGROUND The development of thyroid carcinoma may be influenced genetically and has been associated with certain HLA alleles. HLA‐C alleles have not been studied in depth, because available serology techniques have not been very reliable in detecting them. However, the development of molecular biology provided an efficient and reliable technique for allele detection. The aims of the current study were to determine whether there is a significant association between any HLA‐C allele and differentiated thyroid carcinoma and to establish the possible susceptibility or protection alleles related to these tumors. METHODS HLA‐C genotyping was performed in 63 patients undergoing surgery for differentiated thyroid carcinoma (57 patients with the papillary subtype and 6 patients with the follicular subtype). A representative sample of 100 nonrelated healthy Caucasians was used as a control group from the same geographic area. The polymerase chain reaction–sequence‐specific primer technique was used for HLA‐C genotyping. The clinical variables analyzed were age, gender, family history of thyroid pathology, residence in areas of endemic goiter, asymptomatic status, presence of hyperthyroidism, compressive symptoms, presence of an intrathoracic thyroid component, histologic variables of the tumor, and evolution. The chi‐square test, the Mantel–Haenszel test, and the Bonferroni correction were used for the statistical analysis. RESULTS In the control group, a significant correlation was observed between the lower frequency of the HLA‐Cw7 allele and the development of differentiated thyroid carcinoma (P < 0.05). Analysis of the different clinical variables revealed a relation between HLA‐C alleles and three clinical situations: cervical lymph node involvement with HLA‐Cw7 and HLA‐Cw2, vascular involvement with HLA‐Cw7 and HLA‐Cw1, and cervical carcinoma recurrence with HLA‐Cw1. However, after application of the Bonferroni correction, only the association between HLA‐Cw7 and lymphatic (Pc = 0.0483) or vascular involvement (Pc = 0.0324) persisted. CONCLUSIONS The results revealed a relation between HLA‐Cw7 and differentiated thyroid carcinoma. In future investigations, HLA‐C typing may help to identify patients with a poor prognosis. Cancer 2004;100:264–9. © 2003 American Cancer Society.

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“…Family segregation allowed to define the Cw*070403‐bearing haplotype as follows: A*680102–Cw*070403–B*1518–DRB1*1103–DRB3*020201–DQB1*03AF. Cw*0704 was identified in haplotypes including B*1518 in Oriental populations and associated to B*4402 in Caucasoid and Black people (2, 3). Therefore, the new Cw*070403 variant here described in a Caucasian individual would show probably a strong relation with the Cw*070401 allele found in the B*1518‐bearing oriental haplotype.…”

mentioning

confidence: 64%

Sequencing of four new HLA‐C alleles, Cw070403, Cw0757, Cw1705, and Cw0223

Balas¹,

Bustamante²,

García‐Sánchez³

et al. 2009

Tissue Antigens

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Identification and DNA typing of two Cw7 alleles (Cw∗0702 and Cw∗0704) in Japanese, with the corrected sequence of Cw∗0702

Cited by 15 publications

References 15 publications

Efficient cloning and expression of HLAclass I cDNA in human B‐lymphoblastoidcell lines

Efficient cloning and expression of HLAclass I cDNA in human B‐lymphoblastoidcell lines

Frequency of HLA‐C alleles in differentiated thyroid carcinoma in southeastern Spain

Sequencing of four new HLA‐C alleles, Cw070403, Cw0757, Cw1705, and Cw0223

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Identification and DNA typing of two Cw7 alleles (Cw∗0702 and Cw∗0704) in Japanese, with the corrected sequence of Cw∗0702

Cited by 15 publications

References 15 publications

Efficient cloning and expression of HLAclass I cDNA in human B‐lymphoblastoidcell lines

Efficient cloning and expression of HLAclass I cDNA in human B‐lymphoblastoidcell lines

Frequency of HLA‐C alleles in differentiated thyroid carcinoma in southeastern Spain

Sequencing of four new HLA‐C alleles, Cw*070403, Cw*0757, Cw*1705, and Cw*0223

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Sequencing of four new HLA‐C alleles, Cw070403, Cw0757, Cw1705, and Cw0223