Identification and discrimination of extracellularly active cathepsins B and L in high-invasive melanoma cells

Investigation on the anti-tumor efficacy by expression of GPI-anchored mIL-21 on the surface of B16F10 cells in C57BL/6 mice

“…Based on these researches and our previous works (Jun et al 2004(Jun et al , 2007(Jun et al , 2008a, we investigated if the expression of cytokines on the surface of B16F10 cells surface as a GPI-anchored form was effective in inducing anti-tumor immune response. Due to B16F10 cells characterized by low immunogenicity and the highly invasive melanoma cell line, it forms lethal subcutaneous tumors, with no metastasis to the lung unless injected intravenously, and the B16F10 cells is also highly malignant tumorigenicity in mice model (Klose et al 2006). Accordingly, The experiment results from our study showed that after the B16F10 cells were inoculated into mice, the growth rate and tumor expansion were so fast that all nine mice generated tumors in 20 days (Fig.…”

Cystatins as regulators of cancer

“…Other protease families show increased expression in cancer cells, but the cathepsins have multiple roles which relate to the aggressiveness of most cancers. While cathepsins are normally lysosomal, cancer cells often overexpress these enzymes on the cell surface and/or secrete them (Klose et al, 2006). The cancer cell associated cathepsins are involved in many cancer cell functions including extracellular matrix (ECM) degradation, invasion, proliferation, and angiogenesis (Gocheva et al, 2006).…”

Oxygen–glucose deprivation (OGD) and interleukin-1 (IL-1) differentially modulate cathepsin B/L mediated generation of neuroprotective perlecan LG3 by neurons

“…The cathepsin L selective inhibitor, Z-FY-CHO has previously been shown to be membrane permeable (Klose et al, 2006; Pacheco et al, 2005) and may, therefore, act both outside and inside the cell. Hence, the results presented here do not distinguish extracellular cathepsin L activity from intracellular activity.…”

“…Cultures were treated with 10 μM of Z-FY-CHO or CA074, a concentration range which has previously been shown to be active (Klose et al, 2006; Matarrese et al, 2010). Control cultures were treated with an equal volume of vehicle, DMSO.…”