Identification and differentiation of human schistosomes by polymerase chain reaction

Kato-Hayashi, Naoko; Kirinoki, Masashi; Iwamura, Yukio; Kanazawa, Tamotsu; Kitikoon, Viroj; Matsuda, Hajime; Chigusa, Yuichi

doi:10.1016/j.exppara.2009.11.008

Cited by 50 publications

(36 citation statements)

References 26 publications

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“…The initial detection cycle threshold (C t ) of DNA in first four samples was 39.8-38.24 (Log quantity was 2.6-3.1 IU/ml). This result agrees more or less with Kato-Hayashi et al (2010) who found that schistosome DNA (S. mansoni, S. haematobium, S. japonicum, and S. mekongi) could be detected from day 1 p.i. in experimentally infected animals.…”

Section: Resultssupporting

confidence: 91%

Diagnostic and prognostic value of cell free circulating Schistosoma mansoni DNA: an experimental study

Eraky

Aly

2014

J Parasit Dis

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Searching for a more sensitive and accurate marker for schistosomiasis diagnosis and treatment follow up is a potential necessity. Hereby, we evaluated usefulness of circulating free DNA as a marker for schistosomiasis diagnosis, assessing drug efficacy and monitoring the control interventions impact using SYBR green real-time PCR. A batch of mice were infected by 90 ± 10 Schistosoma mansoni cercariae. Starting from the 2nd day post infection (p.i.), groups of 2 or 3 mice were sacrificed every 3 days until 30 days p.i. The remaining animals were treated by a single dose of 400 mg/kg mefloquine and sacrificed in group at 5, 10, 21 days post treatment (35, 40, 51 days p.i.). Using SYBR green real time qPCR, pooled sera DNA were extracted and amplified. The results showed that, circulating free S. mansoni DNA was detected from the 2nd day post infection (p.i.) onwards with gradual decrease in the cycle threshold value C t which indicates the gradual elevation of the DNA level (Log quantity was 2.6-3.1 IU/ml), As the infection progressed, DNA quantity was increased(Log quantity was 6.29 IU/ml). Initial increase of circulating free DNA was observed 10 days post treatment (40 days p.i.) (Log quantity was 7.38 IU/ ml). That was followed by a progressive decrease in DNA level by the end of 21st day, post treatment (51 p.i.) (Log quantity 4.35 IU/ml). In conclusion, circulating free S. mansoni DNA is a reliable marker in the diagnosis of schistosomiasis and for assessing drug efficacy and monitoring the impact of control interventions.

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Section: Resultssupporting

confidence: 91%

Diagnostic and prognostic value of cell free circulating Schistosoma mansoni DNA: an experimental study

Eraky

Aly

2014

J Parasit Dis

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“…Indeed, PCR identification of S. mansoni in urine sediments has proved superior in diagnostic accuracy to the KK and urine CCA tests in areas of endemicity (224). PCR-based tests can detect CFPD in host serum from a very early schistosome infection (225,226), even in the first week postinfection (227), thus representing a useful adjunct for the early diagnosis of schistosomiasis. In combination with real-time PCR, the approach has proven valuable for monitoring therapeutic responses (222,228), as the amount of CFPD declines gradually following effective treatment.…”

Section: Detection Of Cell-free Parasite Dna In Serum and Other Body mentioning

confidence: 99%

Advances in the Diagnosis of Human Schistosomiasis

et al. 2015

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SUMMARY Schistosomiasis is a major neglected tropical disease that afflicts more than 240 million people, including many children and young adults, in the tropics and subtropics. The disease is characterized by chronic infections with significant residual morbidity and is of considerable public health importance, with substantial socioeconomic impacts on impoverished communities. Morbidity reduction and eventual elimination through integrated intervention measures are the focuses of current schistosomiasis control programs. Precise diagnosis of schistosome infections, in both mammalian and snail intermediate hosts, will play a pivotal role in achieving these goals. Nevertheless, despite extensive efforts over several decades, the search for sensitive and specific diagnostics for schistosomiasis is ongoing. Here we review the area, paying attention to earlier approaches but emphasizing recent developments in the search for new diagnostics for schistosomiasis with practical applications in the research laboratory, the clinic, and the field. Careful and rigorous validation of these assays and their cost-effectiveness will be needed, however, prior to their adoption in support of policy decisions for national public health programs aimed at the control and elimination of schistosomiasis.

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“…Parasite-specific antibody detection by enzyme-linked immunosorbent assay has a high sensitivity and is useful for mass screening, but it has the limitation of a relatively low specificity due to cross-reactivity, and it does not always correlate with an active infection (Doenhoff et al 2004). The detection of parasite DNA in host-derived material is direct evidence of an actual infection, which is why molecularbased approaches to detect S. japonicum DNA, such as conventional polymerase chain reaction (c-PCR) and loop-mediated isothermal amplification, have been developed (Xia et al 2009, Kato-Hayashi et al 2010, Xu et al 2010. Recently, a SYBR ® green-based real-time PCR (Lier et al 2006(Lier et al , 2008, a minor groove-binding probebased real-time PCR (Lier et al 2009), and a TaqMan probe-based real-time PCR (Hung & Remais 2008) were published as sensitive and fast methods that could quantify specific S. japonicum DNA in biological samples and in environmental water.…”

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confidence: 99%

Molecular detection of Schistosoma japonicum in infected snails and mouse faeces using a real-time PCR assay with FRET hybridisation probes

Thanchomnang

Intapan

Sri-Aroon³

et al. 2011

Mem. Inst. Oswaldo Cruz

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Identification and differentiation of human schistosomes by polymerase chain reaction

Cited by 50 publications

References 26 publications

Diagnostic and prognostic value of cell free circulating Schistosoma mansoni DNA: an experimental study

Diagnostic and prognostic value of cell free circulating Schistosoma mansoni DNA: an experimental study

Advances in the Diagnosis of Human Schistosomiasis

Molecular detection of Schistosoma japonicum in infected snails and mouse faeces using a real-time PCR assay with FRET hybridisation probes

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