Identification and differential expression of human carcinoma-associated antigens in hepatocellular carcinoma tissues

Abstract

This study was designed to identify and verify hepatocellular carcinoma (HCC)-associated human carcinoma antigens (HCAs) that may be useful as tumor markers for HCC. We found that BCE075 and BCD021 anti-HCA antibodies were immunostained in the liver tissue samples and showed specific staining. Their expression was increased in HCC compared with normal liver tissues (P = 0.008). Immunoprecipitation and mass spectrometry analyses of the proteins precipitated by these two antibodies were identified to be cytoskeleton-associated protein 4 (CLIMP63) and brain-type glycogen phosphorylase (PYGB). This study demonstrated that HCC tissues expressed specific HCA glycoproteins, suggesting that our mouse monoclonal anti-HCA antibodies could be useful for immunohistochemical analysis of HCA expression as potential biomarkers for HCC diagnosis.

Glycogen Phosphorylase B Is Regulated by miR101-3p and Promotes Hepatocellular Carcinoma Tumorigenesis

“…High PYGB expression was positively correlated with poor prognosis of ovarian cancer patients (Zhou et al, 2019). Here, we found that mRNA expression levels of PYGB were enhanced in tumor tissues such as HCC, prostate adenocarcinoma (PRAD), colon cancer (COAD), lung cancer (LUAD) and lung squamous cell carcinoma (LUSC), consistent with the results reported by previous studies (Uno et al, 1998;Tashima et al, 2000;Lee et al, 2006;Zhou et al, 2013;Wang et al, 2018). Moreover, we further confirmed the high mRNA and protein expression of PYGB using GEO datasets and ZZU HCC cohort, respectively.…”

“…Dysregulated expression of PYGB in a series of cancers was discovered in last two decades (Uno et al, 1998;Tashima et al, 2000;Lee et al, 2006;Wang et al, 2018). In HCC, mouse monoclonal anti-HCA antibody staining indicated PYGB could be served as a potential biomarker for HCC diagnosis (Zhou et al, 2013), which showed the high expression of PYGB in HCC. In this study, we focused on the function and regulatory mechanism of PYGB in HCC development and metastasis.…”

“…More importantly, PYGB overexpression was found during the conversion process from adenoma cells into carcinoma cells, which indicates that PYGB may be served as a prognostic biomarker for cancer from precancerous lesions (Uno et al, 1998;Tashima et al, 2000). In HCC, mouse monoclonal anti-Human Carcinoma antigen (HCAs) antibody specifically stained PYGB in HCC tissues, indicating that PYGB could be served as a potential biomarker for HCC diagnosis (Zhou et al, 2013). However, the detailed roles of PYGB in HCC, as well as the regulatory mechanisms, are still unclear.…”