Identification and differential expression of two dehydrin cDNAs during maturation of Jatropha curcas seeds

Omar, Samar; Elsheery, Nabil I.; Kalaji, Hazem M.; Ebrahim, Mohsen K. H.; Pietkiewicz, S.; Lee, C. H.; Allakhverdiev, Suleyman I.; Xu, Zeng-Fu

doi:10.1134/s0006297913050076

Cited by 14 publications

(12 citation statements)

References 22 publications

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“…In some plants, only one DHN gene is available, e.g., OsDhn1 in rice (Lee et al, 2005). On the other hand, multiple DHN genes were identified in other plant species, such as Jatropha curcas, which has 2 genes, JcDHN-1 and JcDHN-2 (Omar et al, 2013). Close (1996) classified plant DHNs into 5 classes: Y n SK n , Y n K n , SK n , K n , and K n S. However, the domain organization of the DHN protein family (PF00257) revealed 12 distinguishable architectures according to the Pfam protein families database (Punta et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Characterization of dehydrin AhDHN from Mediterranean saltbush (Atriplex halimus)

Sadder¹,

Al‐Doss²

2014

Turk J Biol

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IntroductionAtriplex spp. (saltbush) is a xerohalophyte that belongs to the subfamily Chenopodiaceae and family Amaranthaceae (http://www.mobot.org/MOBOT/research/APweb/). Saltbush is able to produce huge biomass (Glenn et al., 1999). In addition, it has been utilized as livestock fodder with good protein content (Khan et al., 2000). Natural populations of the Mediterranean saltbush (A. halimus L.) are distributed in subhumid and arid regions including North Africa and the Arabian Peninsula (Le Houerou, 1992;Al-Turki et al., 2000; http://ww2.bgbm. org/herbarium/). They have evolved many adaptation mechanisms and physiological responses to cope with different abiotic stresses (Sadder et al., 2013).Water deficit is a major stress caused by limited water availability in soil; however, it can also be induced by salt stress. Generated stress signals usually activate controlling pathways in plants for ionic/osmotic homeostasis and detoxification (Rodríguez-Milla and Salinas, 2009;Li et al., 2013). Dehydrins (DHNs) are plant proteins that belong to the "late embryogenesis abundant II" group. They play a fundamental role in plant response and adaptation to multiple abiotic stresses (Hanin et al., 2011). DHNs were found to be associated with drought stress in olives (Tripepi et al., 2011), with osmotic stress in tea (Paul and Kumar, 2013) and with salt stress in Physcomitrella patens (Ruibal et al., 1012) and tomatoes (Muñoz-Mayor et al., 2012). In addition, they were found associated with cold stress in soybean (Yamasaki et al., 2013) and spinach (Chen et al., 2012).DHNs contain several hydrophilic residues. Any reduction in hydration or increase in compatible solutes can lead to conformational and functional changes (Danyluk et al., 1998;Hanin et al., 2011). DHN in α-helical conformation displays amphipathic protein-protein or protein-membrane interactions. Therefore, DHN can protect other proteins from further loss of water envelope (Koag et al., 2003). Furthermore, DHNs can elevate tolerance through radical-reducing activity, where H, R, and other reactive residues on their surfaces exhibit reactive oxygen species (ROS) scavenging ability (Hanin et al., 2011). The interactions of a residue with ROS can lead to oxidation of the residue, and thus DHNs can function as antioxidants (Hara et al., 2013). Moreover, DHNs can maintain cellular structure by acting as space-fillers between intracellular complexes (Tunnacliffe and Wise, 2007). This study was carried out to clone and characterize a novel DHN gene from A. halimus.

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Section: Discussionmentioning

confidence: 99%

Characterization of dehydrin AhDHN from Mediterranean saltbush (Atriplex halimus)

Sadder¹,

Al‐Doss²

2014

Turk J Biol

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“…Only DHN1a_s was reported to be involved in resistance to cold and drought as well as the growth of Botrytis cinerea (Rosales et al, 2014). There are two forms of DHN in Jatropha curcas seeds, JcDHN_1 (Y 3 SK 2 ) and JcDHN_2 (Y 2 SK 2 ); the transcript level change of JcDHN_2 was 8-fold that of JcDHN_1 at its maximum value, a time when the water content of the seed changed dramatically from 42% for mature seeds to 12% for desiccated seeds (Omar et al, 2013). In addition, LEA proteins might function as molecular chaperones (Wise and Tunnacliffe, 2004) to help non-natural proteins resist aggregation in vitro (Goyal et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

The K-segments of wheat dehydrin WZY2 are essential for its protective functions under temperature stress

Yang

Zhang

et al. 2015

Front. Plant Sci.

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Dehydrins (DHNs), group 2 of late embryogenesis abundant (LEA) proteins, are up-regulated in most plants during cold, drought, heat, or salinity stress. All DHNs contain at least one K-segment, which is believed to play a significant role in DHN function by forming an amphipathic helix. In previous studies, wzy2, an YSK2-type DHN gene, was isolated from the Zhengyin 1 cultivar of Triticum aestivum under cold and drought stress treatment conditions. Four WZY2 truncated derivatives were constructed to knock out the K-, Y- or S-segment, which potentially affect the function of the protein. In vivo assays of Escherichia coli viability enhancement, in vitro lactate dehydrogenase (LDH) activity protection and ex vivo protein aggregation prevention assays revealed that WZY2 acted as a protectant and improved stress tolerance during temperature variation. The results also showed that unlike the truncated derivative without K-segments, the derivative containing two K-segments had remarkable effects that were similar to those of full-length WZY2, indicating that the K-segment is the major functional component of WZY2. Moreover, compared with the other segments, the first K-segment might be the most critical contributor to WZY2 functionality. In general, this work highlights the behavior of DHNs in relieving cold stress ex vivo and the contribution of the K-segment to DHN function.

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“…MDA is the decay product of poly unsaturated fatty acids of biomembranes. Increasing of H 2 O 2 and subsequently MDA contents clearly indicate the oxidative status of the cell (Omar et al, 2013). Thus, oxidative stress may be one of the potential mechanisms by which harmful effect of herbicide is occurred (Bongiovanni et al, 2012;Hassan and Alla, 2005).…”

Section: Changes In H 2 O 2 and Mda Valuesmentioning

confidence: 97%

Nano Selenium: Reduction of severe hazards of Atrazine and promotion of changes in growth and gene expression patterns on Vicia faba seedlings

Zedan¹,

Omar²

2019

Afr. J. Biotechnol.

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Atrazine is a widely used herbicide on many crops and is considered one of water pollutant with approved biological hazards on plants, animals and human. Vicia faba seedlings as a biological system, is used to investigate the genotoxicity of Atrazine. Also, Nano selenium (N-Se), a Nano particle with reactive oxygen species scavenging activity was applied to reduce the genotoxicity of Atrazine. Atrazine treatment at concentration of 35 mg/L is applied. Two concentrations of N-Se (10 and 20 ppm) were used alone and in combination with Atrazine (35 mg/L) in addition to control treatment. Changes in germination percentage, shoot and root length, hydrogen peroxide (H 2 O 2) content, lipid peroxidation product malondialdehyde (MDA), chromosomal aberrations, and mitotic index were determined. Semi quantitative RT-PCR analysis (sqRT-PCR) was applied to investigate associated changes in expression pattern of some stress related genes such as antioxidant enzymes, heat shock proteins (HSP17.9, HSP70.1), photosystem II (PSII) and Metalothioniene (MT). Atrazine treatment recorded the lowest germination percentage and caused a reduction in shoot and root length. Significant increase in H 2 O 2 value, MDA contents, and chromosomal abnormalities percent resulted in Atrazine treatment. Noticeable suppression in expression level of all studied genes was accompanied with Atrazine treatment. N-Se, in its two concentrations with Atrazine causing a reduction in all severe effects of Atrazine and improving seedlings performance. Treatments with N-Se induced the expression of MT gene with increase in expression level alongside increase in the concentration of N-Se. This is one of rare studies that investigate the biological effects of N-Se in vivo anti-mutagenesis of Atrazine as well as a first record of Nano metalic particles N-Se as inducer for MT genes.

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Identification and differential expression of two dehydrin cDNAs during maturation of Jatropha curcas seeds

Cited by 14 publications

References 22 publications

Characterization of dehydrin AhDHN from Mediterranean saltbush (Atriplex halimus)

Characterization of dehydrin AhDHN from Mediterranean saltbush (Atriplex halimus)

The K-segments of wheat dehydrin WZY2 are essential for its protective functions under temperature stress

Nano Selenium: Reduction of severe hazards of Atrazine and promotion of changes in growth and gene expression patterns on Vicia faba seedlings

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