Identification and differential expression analysis of anthocyanin biosynthetic genes in root-skin color variants of radish (Raphanus sativus L.)

Development of Molecular Markers for Predicting Radish (Raphanus sativus) Flesh Color Based on Polymorphisms in the RsTT8 Gene

“…In B. juncea, an insertion of 1279 bp in exon seven of the TT8 gene disrupts its function, and the presence of a C→T SNP in exon seven results in the production of a truncated protein. On the other hand, upregulation of RsTT8 is associated with red skin or red flesh color in radish [33,37].…”

“…Recently, several research groups performed transcriptomic analysis to identify the genes controlling anthocyanin accumulation in radish [ 3 , 33 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 ] and found that major genes and their regulatory transcription factors involved in the flavonoid biosynthetic pathway are essential for anthocyanin accumulation. However, none of these genes represents a novel regulatory gene that controls anthocyanin biosynthesis in a spatiotemporal or tissue-specific manner.…”

“…In B. juncea , an insertion of 1279 bp in exon seven of the TT8 gene disrupts its function, and the presence of a C→T SNP in exon seven results in the production of a truncated protein. On the other hand, upregulation of RsTT8 is associated with red skin or red flesh color in radish [ 33 , 37 ]. Similar to the results of the current study, four SNPs found in the first three exons of BoTT8 are associated with green or purple color in broccoli ( B. oleracea var.…”

“…The defective expression of TT8 caused by either transposable element (TE) insertion or mutation has been shown to affect anthocyanin accumulation in Brassica species, including B. rapa [ 34 ], B. juncea [ 35 ], and B. oleracea [ 36 ]. In radish, although the upregulation of RsTT8 expression has been associated with red skin or red flesh [ 33 , 37 ], polymorphisms in RsTT8 associated with radish skin or flesh color have not yet been identified.…”

Differential Regulation of Anthocyanins in Cerasus humilis Fruit Color Revealed by Combined Transcriptome and Metabolome Analysis

“…For example, Xu et al [31] found key genes responsible for betalain-/anthocyanidin-production in bougainvilleas by transcriptome sequencing. Similarly, Yu et al [32] illustrated the molecular regulatory mechanism of radish formation by transcriptome sequencing. Additionally, metabolomics analysis could be regarded as a technical means of association analysis, together with other data, to analyze the function of genes involved in the metabolic pathway of interest and also provide powerful supporting information for gene mining [33][34][35].…”