Identification and developmental profiles of hexamerins in antenna and hemolymph of the honeybee, Apis mellifera

Danty, Emmanuelle; Arnold, Gérard; Burmester, Thorsten; Huet, Jean‐Claude; Huet, D.; Pernollet, Jean‐Claude; Masson, C.

doi:10.1016/s0965-1748(98)00011-3

Cited by 53 publications

(60 citation statements)

References 49 publications

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“…Also, we showed that vitellogenin is a preferred target of oxidative carbonylation in comparison with apolipoprotein 1 and hexamerin, which similar to vitellogenin are major hemolymph proteins in adult bees (56)(57)(58). Preferential carbonylation has been described for Cu,Zn-superoxide dismutase (59, 60), a key antioxidant enzyme with metal-binding characteristics that are comparable with properties of vitellogenin.…”

Section: Discussionmentioning

confidence: 76%

Reproductive protein protects functionally sterile honey bee workers from oxidative stress

Seehuus

Norberg

Gimsa

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

511

468

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Research on aging shows that regulatory pathways of fertility and senescence are closely interlinked. However, evolutionary theories on social species propose that lifelong care for offspring can shape the course of senescence beyond the restricted context of reproductive capability. These observations suggest that control circuits of aging are remodeled in social organisms with continuing care for offspring. Here, we studied a circuit of aging in the honey bee (Apis mellifera). The bee is characterized by the presence of a long-lived reproductive queen caste and a shorter-lived caste of female workers that are life-long alloparental care givers. We focus on the role of the conserved yolk precursor gene vitellogenin that, in Caenorhabditis elegans, shortens lifespan as a downstream element of the insulin͞insulin-like growth factor signaling cascade. Vitellogenin protein is synthesized at high levels in honey bee queens and is abundant in long-lived workers. We establish that vitellogenin gene activity protects worker bees from oxidative stress. Our finding suggests that one mechanistic explanation for patterns of longevity in bees is that a reproductive regulatory pathway has been remodeled to extend life. This perspective is of considerable relevance to research on longevity regulation that builds largely on inference from solitary model species.aging ͉ social evolution ͉ vitellogenin ͉ carbonylation ͉ RNA interference

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Section: Discussionmentioning

confidence: 76%

Reproductive protein protects functionally sterile honey bee workers from oxidative stress

Seehuus

Norberg

Gimsa

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

511

468

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“…Overall, four hexamerin genes, Hex70a, Hex70b, Hex70c, and Hex110, were identified in the honeybee genome (Martins et al 2010). Protein products of these genes were identified in the hemolymph of different stages of the honeybee via 1D-E (Danty et al 1998;Cunha et al 2005;Martins and Bitondi 2012). All of the four hexamerins have been identified by 2D-E in red-eye pupal hemolymph (Erban et al 2014).…”

Section: Hexamerin-storage or Transport Protein?mentioning

confidence: 99%

“…The abundance of the hexamerin 70a subunit does not apparently change, at least until the 7th day of adult life (Cunha et al 2005). Danty et al (1998) showed that hexamerin 70a is the only hexamerin present in a large amount in hemolymph of later adult honeybee worker stages. Hexamerins 70b and 70c exhibit a similar developmental profile because they disappear from the hemolymph with worker emergence, while hexamerin 110 disappears earlier than 70b and 70c (Danty et al 1998).…”

Section: Hexamerin-storage or Transport Protein?mentioning

confidence: 99%

“…Danty et al (1998) showed that hexamerin 70a is the only hexamerin present in a large amount in hemolymph of later adult honeybee worker stages. Hexamerins 70b and 70c exhibit a similar developmental profile because they disappear from the hemolymph with worker emergence, while hexamerin 110 disappears earlier than 70b and 70c (Danty et al 1998). Bitondi et al (2006) found that hexamerin 110 subunits are highly abundant in larval hemolymph, but are decreased at the larval spinning stage, and remain at low levels in pupae and adults (Bitondi et al 2006).…”

Section: Hexamerin-storage or Transport Protein?mentioning

confidence: 99%

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Detailed proteome mapping of newly emerged honeybee worker hemolymph and comparison with the red-eye pupal stage

et al. 2016

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-The honeybee, Apis mellifera , undergoes complete metamorphosis before transitioning to the adult stage. The newly emerged individual and the red-eye pupa stage are well defined and easily recognizable in the time life cycle honeybee and, therefore, very useful for studying physiological and developmental factors. We analyzed in detail the hemolymph proteome of newly emerged honeybee worker using 2D-E-MS/MS (pI 3-10 and 4-7). The comparison of identical hemolymph volumes (20 μL per 2D-E) for newly emerged bee and red-eye pupa revealed a dramatic decrease in the number of spots (qualitative changes) and overall protein quantity during the non-feeding stage. The results increase our knowledge about honeybee metamorphosis during the non-feeding period and clarify previous findings regarding particular proteins. The results will be useful for future comparative physiological, developmental, and host-pathogen studies on individual or population level.Apis mellifera / hemolymph / metamorphosis / hexamerin / ferritin

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“…Hexamerins store amino acids accumulated during larval development until they are used during metamorphosis or by the adult insects (27). Recently, Danty et al (28) used antibodies to Drosophila larval storage proteins to reveal several hexamerins present in the hemolymph and antennal tissue of honey bees. Two of these hexamerins persisted longer in adult queens than in workers, suggesting caste differences in synthesis and utilization.…”

mentioning

confidence: 99%

Differential gene expression between developing queens and workers in the honey bee,Apismellifera

Evans

Wheeler

1999

Proc. Natl. Acad. Sci. U.S.A.

280

200

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Many insects show polyphenisms, or alternative morphologies, which are based on differential gene expression rather than genetic polymorphism. Queens and workers are alternative forms of the adult female honey bee and represent one of the best known examples of insect polyphenism. Hormonal regulation of caste determination in honey bees has been studied in detail, but little is known about the proximate molecular mechanisms underlying this process, or any other such polyphenism. We report the success of a molecular-genetic approach for studying queen-and workerspecific gene expression in the development of the honey bee (Apis mellifera). Numerous genes appear to be differentially expressed between the two castes. Seven differentially expressed loci described here belong to at least five distinctly different evolutionary and functional groups. Two are particularly promising as potential regulators of caste differentiation. One is homologous to a widespread class of proteins that bind lipids and other hydrophobic ligands, including retinoic acid. The second locus shows sequence similarity to a DNAbinding domain in the Ets family of transcription factors. The remaining loci appear to be involved with downstream changes inherent to queen-or worker-specific developmental pathways. Caste determination in honey bees is typically thought of as primarily queen determination; our results make it clear that the process involves specific activation of genes in workers as well as in queens.

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Identification and developmental profiles of hexamerins in antenna and hemolymph of the honeybee, Apis mellifera

Cited by 53 publications

References 49 publications

Reproductive protein protects functionally sterile honey bee workers from oxidative stress

Reproductive protein protects functionally sterile honey bee workers from oxidative stress

Detailed proteome mapping of newly emerged honeybee worker hemolymph and comparison with the red-eye pupal stage

Differential gene expression between developing queens and workers in the honey bee,Apismellifera

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