Identification and development of a functional marker from 6-SFT-A2 associated with grain weight in wheat

Abstract

As a class of water-soluble, fructose-based oligo- and polysaccharides, fructans are major nonstructural carbohydrates and an important carbon source for grain filling in wheat (Triticum aestivum L.). Four enzymes are involved in fructan synthesis in higher plants, and 6-SFT is a key enzyme in fructan biosynthesis. In this study, thirteen single nucleotide polymorphisms were detected in 6-SFT-A2 in 24 wheat accessions, forming three haplotypes. Two cleaved amplified polymorphic sequence markers developed based on polymorphisms at sites 1870(A-G) and 1951(A-G) distinguished the three haplotypes. 6-SFT-A2 was located on chromosome 4A, between markers P2454.3 and P3465.1 in a doubled haploid (DH) population derived from the cross Hanxuan 10 × Lumai 14. The DH population comprising 150 lines and a historical population consisting of 154 accessions were used in a 6-SFT-A2 marker–trait association analysis. The three haplotypes were significantly associated with thousand-grain weight (TGW) under rainfed conditions. HapIII had a significant positive effect on TGW. There were significant differences between the Hanxuan 10 and Lumai 14 genotypes in both rainfed and irrigated environments. The average TGW of Lumai 14 (HapIII) was higher than that of Hanxuan 10 (HapI). The frequencies of 6-SFT-A2HapIII in cultivars released at different periods showed that it had been strongly positively selected in breeding programs. The preferred HapIII for TGW occurred at higher frequencies in Gansu, Beijing, Shanxi, and Hebei than other regions in northern China.Electronic supplementary materialThe online version of this article (doi:10.1007/s11032-015-0266-9) contains supplementary material, which is available to authorized users.

A dCAPS marker developed from a stress associated protein gene TaSAP7-B governing grain size and plant height in wheat

“…Single nucleotide polymorphism (SNP) is a solo nucleotide variation that occurs at a specific position in the genomes, and was recently developed as genetic markers (Garces-Claver et al 2007;Zhang et al 2013). As the 3rd generation molecular marker, SNPs were used as a tool for a series of research program, such as, marker-assisted selection, genome mapping and association mapping (Rounsley and Last 2010;Yue et al 2015). Recent developments in bioinformatics and sequencing technology are rapidly promoting SNP development in wheat progressively achievable (Winfield et al 2012).…”

Identification of a Novel Allele of TaCKX6a02 Associated with Grain Size, Filling Rate and Weight of Common Wheat

“…Identification of genes for grain traits in wheat is receiving increased attention of researchers. At present, some genes for grain size and weight are known, including TaGW2 [ 59 – 61 ], TaCKX6 [ 30 ], TaGS-D1 [ 62 ], 6-SFT-A2 [ 63 ]. In our study, TaCKX6a02 is also confirmed to tightly relate to grain size and weight by QTL and association analysis.…”

Functional Analysis and Marker Development of TaCRT-D Gene in Common Wheat (Triticum aestivum L.)

“…It is difficult to use the difference between the homologous sequences to distinguish the different genomic distribution of the target gene in common wheat, and to perform the chromosomal localization and precise genetic mapping. In recent years, however, a number of SNP markers in wheat were developed, and further used for mapping and cloning of the related genes ( Su et al, 2011 ; Zhang et al, 2014 ; Yue et al, 2015 ; Li et al, 2016 ). Sequence polymorphism of the target gene among different genomes in wheat was usually used to develop the genome-specific primers.…”