The effect of indigenous and commercial S. cerevisiae yeasts on fermentation and flavour compounds of wines was examined in pasteurised grape juice. The flavour compounds were analysed and identified by GC-FID and GC-MS, respectively and in general, the amounts of these volatiles were increased by the use of both indigenous and commercial yeasts. The levels of isoamyl alcohol, isoamyl acetate, ethyl octanoate and ethyl deconoate exceeded flavour thresholds. All grape juices were fermented to dryness. Selected yeasts produced higher ethanol concentrations compared to spontaneous fermentations.Key words: cv. Kalecik karas¿, fermentation, flavour compounds, Saccharomyces cerevisiae, wine, yeast.
-2863(9'8-32Wine fermentation has traditionally depended on the activity and growth of indigenous yeasts originating from grapes and winery equipment (i.e. natural or spontaneous fermentation) 30 . In enology selected indigenous local, or commercial Saccharomyces cerevisiae strains, can be used to obtain better quality wine than with only natural fermentation 7,30 . Inoculation of grape must with selected yeasts offers better control of alcoholic fermentation and of desirable sensory properties 13,37 . In addition to the main products of ethanol and carbon dioxide, yeasts produce a wide range of flavour compounds during the alcoholic fermentation of grape must. Higher alcohols, esters and acids are quantitatively abundant in wine flavours 19,25 . Previous studies have shown that the yeasts, which induce the fermentative flavour, are responsible for the great differences in the chemical and sensory properties of wines 5,20,21,24,28,36 . Kalecik karas¿ is a native Turkish grape variety of Vitis vinifera. It is a major black grape variety of the Ankara district and is used for the production of good quality red Kalecik karas¿ wine 1,18 . The aim of this study was to investigate differences in fermentation progress and flavour production, using indigenous and commercial S. cerevisiae yeasts, in pasteurised cv. Kalecik karas¿ grape juice, together with spontaneous fermentations for comparison.
1%8)6-%07 %2( 1)8,3(7 =IEWXWThe yeast strain referred to as indigenous S. cerevisiae in this text was selected according to its technological properties from the wine yeasts isolated and identified as S. cerevisiae during cv. Kalecik karas¿ wine fermentations in 1998. The identification of the yeasts was carried out according to API 32C Auxanogramm and API Lab Software (bioMerieux API Systems, Marcy-l'Etoile, France) and Barnett et al 7 . The commercial culture of S. cerevisiae (Fermirouge 7303-Gist Brocades) was obtained from INRA, France.
1IHMYQThe cv. Kalecik karas¿ grape juice was used. Grape juice was prepared by the thermovinification method to promote the colour extraction 3 . Healthy grapes (200 kg) from the vineyard of the Kavakl¿dere Winery (Akyurt, Ankara) were transported to the pilot winery of the Department of Food Engineering, University of Çukurova, Adana. They were de-stemmed and crushed. The crushed grapes were held at 65...