Identification and determination of the qualitative composition of nystatin using thin-layer chromatography and high-performance liquid chromatography

Thomas, A. H.; Pharm, B.; Newland, Penny; Quinlan, Gregory J.

doi:10.1016/s0021-9673(00)82369-9

Cited by 16 publications

(8 citation statements)

References 2 publications

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“…Thin-layer chromatography (TLC) of Nystatin was performed on precoated silica gel plates (silica gel 60 TLC plates; Merck) according to Thomas et al (1981). Briefly, the eluent consisted of the lower phase of chloroformmethanol-water (20:22:10, v/v/v).…”

Section: Analysis and Purification Of Nystatinmentioning

confidence: 99%

Self-association of the polyene antibiotic nystatin in dipalmitoylphosphatidylcholine vesicles: a time-resolved fluorescence study

Coutinho¹,

Prieto²

1995

Biophysical Journal

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The interaction between Nystatin and small unilamellar vesicles of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, both in gel (T = 21 degrees C) and in liquid-crystalline (T = 45 degrees C) phases, was studied by steady-state and time-resolved fluorescence measurements by taking advantage of the intrinsic tetraene fluorophore present in this antibiotic. It was shown that Nystatin aggregates in aqueous solution with a critical concentration of 3 microM. The enhancement in the fluorescence intensity of the antibiotic was applied to study the membrane binding of Nystatin, and it was shown that the antibiotic had an almost fivefold higher partition coefficient for the vesicles in a gel (P = (1.4 +/- 0.1) x 10(3)) than in a liquid-crystalline phase (P = (2.9 +/- 0.1) x 10(2)). Moreover, a time-resolved fluorescence study was used to examine Nystatin aggregation in the membrane. The emission decay kinetics of Nystatin was described by three and two exponentials in the lipid membrane at 21 degrees C and 45 degrees C, respectively. Nystatin mean fluorescence lifetime is concentration-dependent in gel phase lipids, increasing steeply from 11 to 33 ns at an antibiotic concentration of 5-6 microM, but the fluorescence decay parameters of Nystatin were unvarying with the antibiotic concentration in fluid lipids. These results provide evidence for the formation of strongly fluorescent antibiotic aggregates in gel-phase membrane, an interpretation that is at variance with a previous study. However, no antibiotic self-association was detected in a liquid-crystalline lipid bilayer within the antibiotic concentration range studied (0-14 microM).

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Section: Analysis and Purification Of Nystatinmentioning

confidence: 99%

Self-association of the polyene antibiotic nystatin in dipalmitoylphosphatidylcholine vesicles: a time-resolved fluorescence study

Coutinho¹,

Prieto²

1995

Biophysical Journal

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“…A TLC method has been published7, 9 for determining impurities in Zn‐BC and NYS and for their separation and determination in pharmaceutical preparations, but no reports were found for simultaneous separation and determination of Zn‐BC or NYS by planar chromatography in real samples such as feedstuffs. In preliminary experiments, several previously reported mobile phases8, 9, 15–19 were tested for separation and analysis of the investigated compounds.…”

Section: Resultsmentioning

confidence: 99%

“…Mobile phases of similar composition—chloroform: methanol:water (20:22:10, v/v/v)9 for NYS by TLC, and chloroform:ethanol:methanol:water (5:3:3:4, v/v/v/v),8 used as the mobile phase for separating Zn‐BC components by counter‐current chromatography—were not suitable for separating mixtures of Zn‐BC and NYS along with the experiments during these studies by HPTLC. Proposed modifications of glacial acetic acid9 for pH modifier, and of 25% NH 3 for TLC separation of Zn‐BC15 in a mixture with neomycin sulfate and polymyxin B sulfate (methanol: n ‐butanol:25% ammonia:chloroform, 14:4:9:12, v/v/v/v) were also not efficient for analysing Zn‐BC and NYS mixtures in this study.…”

Section: Resultsmentioning

confidence: 99%

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Densitometric determination of zinc bacitracin and nystatin in animal feed

Injac¹,

Štrukelj²,

Djordjevic-Milic³

et al. 2008

J Sci Food Agric

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“…TLC and HPLC [9], LC [3], and derivative spectrometry [10] have been used for the identification and determination of NYS.…”

Section: Introductionmentioning

confidence: 99%

Micellar electrokinetic capillary chromatography determination of zinc bacitracin and nystatin in animal feed

Injac

Kac²,

Mlinarič

et al. 2006

J of Separation Science

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An MEKC procedure was developed for the separation of zinc bacitracin (Zn-BC) and nystatin (NYS) in mixtures and in animal feedstuff. The running buffer was 15 mM borate/19 mM phosphate, pH 8.2, containing 20 mM SDS and 10% v/v methanol. Samples were run at 25 degrees C, the applied voltage was 25 kV, and an additional pressure of 5 mbar was applied. Both analytes were detected by UV simultaneously at 215 nm, Zn-BC alone at 192 and 254 nm, and NYS alone at 305 nm. The method was shown to be specific, accurate (recoveries were 100.0 +/- 0.6% and 100.1 +/- 0.6% for Zn-BC and NYS, respectively), linear over the tested range (correlation coefficients 0.9991 and 0.9994), and precise (RSD below 1.3% for both analytes). The method was applied to determine Zn-BC and NYS as additives in animal feed.

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Identification and determination of the qualitative composition of nystatin using thin-layer chromatography and high-performance liquid chromatography

Cited by 16 publications

References 2 publications

Self-association of the polyene antibiotic nystatin in dipalmitoylphosphatidylcholine vesicles: a time-resolved fluorescence study

Self-association of the polyene antibiotic nystatin in dipalmitoylphosphatidylcholine vesicles: a time-resolved fluorescence study

Densitometric determination of zinc bacitracin and nystatin in animal feed

Micellar electrokinetic capillary chromatography determination of zinc bacitracin and nystatin in animal feed

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