Identification and degradation characterization of hexachlorobutadiene degrading strain Serratia marcescens HL1

Li, M.T.; Hao, Linlin; Sheng, L.X.; Xu, Junhua

doi:10.1016/j.biortech.2008.01.048

Cited by 25 publications

(6 citation statements)

References 26 publications

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“…Complete diazinon removal was observed on day 11 when incubated at 25 o C and 30 o C, but on day 13 at 20 o C. Degradation of diazinon was negligible in the control. Therefore, the optimal incubation temperature for DI101 cells to degrade diazinon was between 25 o C and 30 o C. These results were expected, since most members of the family Enterobacteriaceae grow well at 25-37 o C. Similar results reported that the ranges of pH value and temperature for satisfactory growth of Serratia marcescens strain HL1 cells and hexachlorobutadiene degradation were from 7.0 to 8.0, and 25 to 30 o C, respectively [23]. Similar results reported that the addition of Burkholderia sp.…”

Section: Effects Of Inoculum Density Ph and Temperature On Diazinonsupporting

confidence: 76%

Biodegradation of Diazinon by Serratia marcescens DI101 and its Use in Bioremediation of Contaminated Environment

Abo‐Amer¹

2011

J. Microbiol. Biotechnol.

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Four diazinon-degrading bacteria were isolated from agricultural soil by using an enrichment technique. The biochemical analysis and molecular method including RFLP indicated that these isolates were identical, and one strain designated DI101 was selected for further study. Phylogenetic analysis based on 16S rDNA sequencing indicated that the strain DI101 clearly belongs to the Serratia marcescens group. The ability of the strain to utilize diazinon as a source of carbon and phosphorus was investigated under different culture conditions. The DI101 strain was able to completely degrade 50 mg/l diazinon in MSM within 11 days with a degradation rate of 0.226 day-1. The inoculation of sterilized soil treated with 100 mg/kg of diazinon with 10 6 CFU/g DI101 resulted in a faster degradation rate than was recorded in non-sterilized soil. The diazinon degradation rate by DI101 was efficient at temperatures from 25 to 30 o C and at pHs from 7.0 to 8.0. The degradation rate of diazinon was not affected by the absence of a phosphorus supplement, and addition of other carbon sources (glucose or succinate) resulted in the slowing down of the degradation rate. The maximum degradation rate (V max) of diazinon was 0.292 day-1 and its saturation constant (K s) was 11 mg/l, as determined by a Michaelis-Menten curve. The strain was able to degrade diethylthiophosphate-containing organophosphates such as chlorpyrifos, coumaphos, parathion, and isazofos when provided as a source of carbon and phosphorus, but not ethoprophos, cadusafos, and fenamiphos. These results propose useful information for the potential application of the DI101 strain in bioremediation of pesticide-contaminated environments.

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Section: Effects Of Inoculum Density Ph and Temperature On Diazinonsupporting

confidence: 76%

Biodegradation of Diazinon by Serratia marcescens DI101 and its Use in Bioremediation of Contaminated Environment

Abo‐Amer¹

2011

J. Microbiol. Biotechnol.

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“…Besides, HCBD has low mobility in soils due to its high log K ow. These might be the reasons why the concentrations of HCBD at the sampling sites 1 km away from the plant were very low and about 100 times lower than that in the soil within the plant. Correlation analysis was also carried out to examine the factors that might affect the HCBD concentrations in soils.…”

Section: Resultsmentioning

confidence: 63%

“…Hexachlorobutadiene (HCBD), a man-made chlorohydrocarbon, has been listed as a candidate persistent organic pollutant (POP) by the Stockholm Convention in 2011 for its persistence, toxicity, bioaccumulation, and potential long-range transport abilities . It was used as an agricultural fumigant, intermediate in rubber, chlorofluorocarbons and lubricants, hydraulic, heat transfer or transformer liquid, laboratory reagents, as well as wash liquid for removing some hydrocarbons. − HCBD has been detected in various samples such as biota, − food, − water, , leachate, sediment, air, and sewage sludge. − To our knowledge, HCBD is not intentionally produced in large commercial quantities in China but may be unintentionally produced. Thus, investigations on the local or regional contamination and distributions of HCBD in China are urgently needed.…”

Section: Introductionmentioning

confidence: 99%

Levels and Distributions of Hexachlorobutadiene and Three Chlorobenzenes in Biosolids from Wastewater Treatment Plants and in Soils within and Surrounding a Chemical Plant in China

Zhang

Wang

Sun

et al. 2014

Environ. Sci. Technol.

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Although hexachlorobutadiene (HCBD) was recently proposed as a candidate persistent organic pollutant (POP) under the Stockholm Convention, information about its environmental levels and distributions is still very limited. In this work, HCBD was determined in the sewage sludge from 37 wastewater treatment plants (WWTPs) in 23 cities and 17 soils near a chemical plant in China. Three chlorobenzenes (CBs) (1,2,4-trichlorobenzene, 1,2,4,5-tetrachlorobenzene, and hexachlorobenzene) were simultaneously studied to help better understand the environmental behavior of HCBD. Concentrations of HCBD in sludge samples ranged from <0.03 to 74.3 ng/g dry weight (dw) with a median value of 0.30 ng/g dw, which was lower than those of the three CBs. Levels of HCBD were not correlated with capacity of the WWTPs and total organic carbon. For soils, high level of HCBD was found in the sample within the plant, with a rapid decreasing concentration trend with the increase of distance from the plant. It was suspected that releasing as a byproduct during manufacturing of chlorinated chemicals was the primary source of HCBD in the studied location. Further risk assessment indicated that the environmental risk of HCBD to soil organisms and the health risk to employees were very low through soil exposure within the plant.

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“…Conversely, debromination is the most crucial process in the detoxification of BFRs and in the reduction of resistance to aerobic biodegradation of toxic intermediates during subsequent metabolic steps (Janssen et al, 2001). Removal of bromine or chlorine atom(s) frequently renders the molecule more susceptible to complete mineralization (Armenante et al, 1999;Li et al, 2008). The complete microbial mineralization of TBBPA was previously accomplished with two steps: reductive debromination of TBBPA under anaerobic conditions with subsequent mineralization of BPA by a Gram-negative aerobic bacterium (Ronen and Abeliovich, 2000).…”

Section: Introductionmentioning

confidence: 99%

One-step process for debromination and aerobic mineralization of tetrabromobisphenol-A by a novel Ochrobactrum sp. T isolated from an e-waste recycling site

et al. 2011

Bioresource Technology

109

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Identification and degradation characterization of hexachlorobutadiene degrading strain Serratia marcescens HL1

Cited by 25 publications

References 26 publications

Biodegradation of Diazinon by Serratia marcescens DI101 and its Use in Bioremediation of Contaminated Environment

Biodegradation of Diazinon by Serratia marcescens DI101 and its Use in Bioremediation of Contaminated Environment

Levels and Distributions of Hexachlorobutadiene and Three Chlorobenzenes in Biosolids from Wastewater Treatment Plants and in Soils within and Surrounding a Chemical Plant in China

One-step process for debromination and aerobic mineralization of tetrabromobisphenol-A by a novel Ochrobactrum sp. T isolated from an e-waste recycling site

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