Identification and composition of the tonsillar and anal enterococcal and streptococcal flora of dogs and cats

Devriese, Luc; Colque, J.I. Cruz; Herdt, Peter De; Haesebrouck, Freddy

doi:10.1111/j.1365-2672.1992.tb04998.x

Cited by 115 publications

(83 citation statements)

References 21 publications

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“…strains were isolated from almost all tested dogs. E. faecium and E. faecalis were most frequently isolated, in agreement to the results obtained by other studies [15][16][17].…”

Section: Discussionsupporting

confidence: 82%

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Antimicrobial resistance in Enterococcus strains isolated from healthy domestic dogs

Bertelloni

Salvadori

Lotti

et al. 2016

Acta Microbiologica et Immunologica Hungarica

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Enterococci are opportunistic bacteria that cause severe infections in animals and humans, capable to acquire, express, and transfer antimicrobial resistance. Susceptibility to 21 antimicrobial agents was tested by the disk diffusion method in 222 Enterococcus spp. strains isolated from the fecal samples of 287 healthy domestic dogs. Vancomycin and ampicillin minimum inhibitory concentrations (MICs) and high-level aminoglycoside resistance (HLAR) tests were also performed. Isolates showed resistance mainly to streptomycin (88.7%), neomycin (80.6%), and tetracycline (69.4%). Forty-two (18.9%) isolates showed an HLAR to streptomycin and 15 (6.7%) to gentamicin. Vancomycin and ampicillin MIC values showed 1 and 18 resistant strains, respectively. One hundred and thirty-six (61.2%) strains were classified as multidrug resistant and six (2.7%) strains as possibly extensively drug-resistant bacteria. Enterococcus faecium and Enterococcus faecalis were the most prevalent antimicrobial resistant species. Companion animals, which often live in close contact with their owners and share the same environment, represent a serious source of enterococci resistant to several antibiotics; for this reason, they may be a hazard for public health by providing a conduit for the entrance of resistance genes into the community.

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“…strains were isolated from almost all tested dogs. E. faecium and E. faecalis were most frequently isolated, in agreement to the results obtained by other studies [15][16][17].…”

Section: Discussionsupporting

confidence: 82%

“…This result, in agreement to other studies [16], suggests that dogs are not an important reservoir of vancomycin-resistant enterococci (VRE), even though VRE have been isolated from canine feces hypothesizing dogs as a source of infection for their owners [15].…”

Section: Discussionsupporting

confidence: 81%

Antimicrobial resistance in Enterococcus strains isolated from healthy domestic dogs

Bertelloni

Salvadori

Lotti

et al. 2016

Acta Microbiologica et Immunologica Hungarica

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“…Several Enterococcus species, together with some unidentified E. avium-like isolates, have been isolated from canine tonsils (Devriese et al, 1992). Most enterococcal species are known to be associated with the intestines and their presence elsewhere is usually regarded as an indicator of faecal contamination.…”

mentioning

confidence: 99%

Enterococcus hermanniensis sp. nov., from modified-atmosphere-packaged broiler meat and canine tonsils

Koort

Coenye

Vandamme

et al. 2004

International Journal of Systematic and Evolutionary Microbiology

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Isolates 302, 334, 356, 377 and 379, detected in modified-atmosphere-packaged broiler meat, together with strains LMG 12317 T and LMG 13617, detected in dog tonsils, were analysed in a polyphasic taxonomy study, including numerical analysis of ribopatterns and whole-cell protein patterns, 16S rRNA gene sequence analysis, DNA-DNA hybridization and determination of some phenotypic properties. The results indicated that these isolates represent a novel species in the genus Enterococcus. The isolates showed classical phenotypic reactions for the genus Enterococcus with the exception of not possessing the Lancefield group D antigen. Isolates 334, LMG 12317 T and LMG 13617 showed the highest 16S rRNA gene sequence similarity (98?3-99?0 %) to the Enterococcus pallens type strain. In the distance matrix tree based on 16S rRNA gene sequences, the three isolates were located in the Enterococcus avium group with E. pallens as their closest phylogenetic neighbour. Numerical analyses of whole-cell protein patterns and HindIII/EcoRI ribotypes placed all seven isolates together in a single cluster separated from the E. avium group reference strains. The DNA-DNA hybridization level between strains 334 and LMG 12317 T was 93?5 %, confirming that they represent the same species. Low hybridization levels (12-30 %) were, by contrast, obtained with the E. pallens and Enterococcus raffinosus type strains. The name Enterococcus hermanniensis sp. nov. is proposed, with strain LMG 12317 T (=CCUG 48100 T ) as the type strain.Phylogenetically, the genus Enterococcus belongs to the clostridial branch of the Gram-positive bacteria. It was not officially recognized as a separate genus until 1984, when [Streptococcus] faecalis and [Streptococcus] faecium were reclassified as Enterococcus faecalis and Enterococcus faecium, respectively (Schleifer & Kilpper-Bälz, 1984). Since then, the number of enterococcal species described has increased, not only by the species transferred from the group D Streptococcus, but also by the description of several novel species. At the time of writing, over 20 species are classified as enterococci. Based on 16S rRNA gene sequence analyses, four phylogenetic groups (E. faecium, Enterococcus avium, Enterococcus gallinarum and Enterococcus cecorum species groups) have been described (Williams et al., 1991).During a study of lactic acid bacteria (LAB) isolated from the spoilage population in modified-atmosphere-packaged (MAP), marinated, broiler legs, several unidentified LAB isolates were detected (Björkroth et al., 2004). Five isolates (302, 334, 356, 377 and 379) originating from fresh packages showed HindIII ribopatterns resembling the patterns of some known enterococci. The patterns of these five isolates were designated types UIVa and UIVb by Björkroth et al. (2004). When these isolates were initially studied by means of whole-cell protein analysis, they were found to possess similar patterns to two isolates (LMG 12317 T and LMG 13617) originating from canine tonsils. The present polyphasic taxonomic study, i...

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“…Çeşitli hayvanların ve insanların gastrointestinal kanalında, ağzında, genital ve üriner sisteminde ve derisi üzerinde bulunabilirler (4,16). Canlı organizmalar dışında, yüzey sularından, akarsulardan, lağım sularından, topraktan ve çeşitli besin maddelerinden enterokok izolasyonu yapılmıştır (10,13).…”

Section: Introductionunclassified

Balık kökenli Enterococcus faecalis suşlarının antibiyotik dirençlilikleri

KAYA¹

2008

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Özet: İnsan ve hayvanlarda komensal olarak bulunan enterokoklar, belirli koşullarda hastalıklara neden olabilirler. Kültür balıkçılığının gelişmesi ve artması ile balıklarda diğer bakteriyel infeksiyonlarla birlikte enterokokkal infeksiyonlar da büyük bir problem olmaya başlamışlardır. Enterokoklar, doğadaki geniş dağılımları, yüksek düzeyde antibiyotik dirençlilikleri ve neden oldukları infeksiyonlardaki yüksek mortaliteleri ile ilgi çeken mikroorganizmalardır. Bu çalışmada, balıklardan izole edilen Enterokokların vankomisin ve diğer antibiyotiklere karşı dirençliliklerinin belirlenmesi amaçlandı. Çalışmamızda Ege Bölgesinde hasta ve ölü balıklardan izole edilen 26 Enterococcus faecalis suşu kullanıldı. E. faecalis suşlarının siprofloksasin, eritromisin, tetrasiklin, streptomisin, ampisilin, gentamisin, penisilin ve vankomisin duyarlılıkları agar-disk difüzyon tekniği ile incelendi. Vankomisin dirençli enterokokların (VRE) vankomisin direnç fenotipleri agar dilusyon yöntemi ile belirlendi. İncelenen E. faecalis suşları, siprofloksasin, eritromisin, tetrasiklin, streptomisin, ampisilin, gentamisin, penisilin ve vankomisine sırasıyla %34.6, %3.8, %3.8, %96.1, %11.5, %92.3, %11.5 ve %3.8 oranında dirençli bulundu. İncelenen 26 suştan 1'inin (%3.8) vankomisine dirençli olduğu saptandı. Bu suşun vankomisin direnç fenotipinin vanA olduğu belirlendi. Bu bulgular, balıklarda streptomisin, gentamisin ve siprofloksasine dirençli suşların yaygın olarak bulunduğunu; düşük oranda da olsa vankomisine dirençli suş bulunması, balıkların VRE kaynağı olarak dikkate alınması gerektiğini gösterdi.Anahtar sözcükler: Antibiyotik dirençlilik, balık, Enterococcus faecalis, vankomisin Antimicrobial resistance in Enterococcus faecalis from fishesSummary: Enterococci, which are found as commensal in animals and human, can cause infections in definite conditions.The improvement and increment of aquaculture cause to happen a big problem for not only the other bacterial infections but also enterococcal infections in fishes. Enterococci are interesting microorganisms owing the wide range of distribution in nature, high level of antimicrobial resistance, and high mortality rates in enterococcal infections. The aim of this study was to determine the resistance to vancomycin and other antimicrobials in fish isolates of enterococci. Twenty six Enterococcus faecalis strains which were isolated in sick and dead fishes were used in study. Ciprofloxacin, erythromycin, tetracycline, streptomycin, ampicillin, gentamicin, penicillin, and vancomycin susceptibility of E.faecalis strains were examined with agar disc diffusion techniques. The vancomycin resistance phenotype of vancomycin resistant enterococci (VRE) was determined by agar dilution method. Ciprofloxacin, erythromycin, tetracycline, streptomycin, ampicillin, gentamicin, penicillin and vancomycin resistance of E.faecalis strains were found as 34.6%, 3.8%, 3.8%, 96.1%, 11.5%, 92.3%, 11.5%, and 3.8%, respectively. One of the 26 E.faecalis strains (3.8%) was found as vancomycin resistant (VRE) an...

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Identification and composition of the tonsillar and anal enterococcal and streptococcal flora of dogs and cats

Cited by 115 publications

References 21 publications

Antimicrobial resistance in Enterococcus strains isolated from healthy domestic dogs

Antimicrobial resistance in Enterococcus strains isolated from healthy domestic dogs

Enterococcus hermanniensis sp. nov., from modified-atmosphere-packaged broiler meat and canine tonsils

Balık kökenli Enterococcus faecalis suşlarının antibiyotik dirençlilikleri

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