1990
DOI: 10.1128/mcb.10.5.2269
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Identification and comparison of stable and unstable mRNAs in Saccharomyces cerevisiae.

Abstract: We developed a procedure to measure mRNA decay rates in the yeast Saccharomyces cerevisiae and applied it to the determination of half-lives for 20 mRNAs encoded by well-characterized genes. The procedure utilizes Northern (RNA) or dot blotting to quantitate the levels of individual mRNAs after thermal inactivation of RNA polymerase II in an rpbl-1 temperature-sensitive mutant. We compared the results of this procedure with results obtained by two other procedures (approach to steady-state labeling and inhibit… Show more

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Cited by 384 publications
(399 citation statements)
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References 108 publications
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“…The AMTal mRNA decays rapidly with a tj/2 of 3.5 min ( Fig. 2A), slightly faster than previously described (5 min [18,27]). This small difference presumably reflects the more complete transcriptional shutoff achieved through simultaneous thermal and glucose repression as opposed to simply the thermal repression used previously.…”
Section: Resultssupporting
confidence: 55%
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“…The AMTal mRNA decays rapidly with a tj/2 of 3.5 min ( Fig. 2A), slightly faster than previously described (5 min [18,27]). This small difference presumably reflects the more complete transcriptional shutoff achieved through simultaneous thermal and glucose repression as opposed to simply the thermal repression used previously.…”
Section: Resultssupporting
confidence: 55%
“…3A (27). Second, treatment of cells with cycloheximide greatly stabilizes the ALToId mRNA (18). The observation that rare codons are a component of the MIE suggests a means through which translation and decay of the AL4ToLl mRNA may be coupled.…”
Section: Discussionmentioning
confidence: 97%
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“…Northern blots were quantitated by using a Betagen Blot Analyzer (Betagen, Waltham, MA. ; Herrick et al 1990). Data are expressed as the loglo of the percentage of each RNA remaining versus time at 36~ Reproducibility of mRNA decay rate measurements was ---15%.…”
Section: Methodsmentioning
confidence: 99%
“…mRNA decay rates were determined by thermal inactivation of rpb1-1 with the use of a protocol modified from Herrick et al (1990). Cultures of yRP384 carrying either pCD61 or pCD62 were grown in 200 ml of synthetic selective media with 2% glucose at 24°C from an OD 600 of 0.1 to an OD 600 of 0.3-0.4.…”
Section: Rna Analysesmentioning
confidence: 99%