Identification and comparison of biological characteristics and pathogenicity of different mating types of V. dahliae isolated from potato and sunflower

Abstract

Potato is one of the most important staple crops in the world. China is one of the leading producers of potatoes, but the industry faces soilborne diseases such as Verticillium wilt. Most potato planting areas in China rotate the crop with sunflower which is also highly susceptible to Verticillium wilt. The comparison of the biological characteristics and pathogenicity of different mating types of Verticillium dahliae isolated from potato and sunflower in the major planting regions in China is of great importance. This is to help unravel the diversity in V. dahliae population and the sudden increase in infected fields. The diseased samples collected were cultured on PDA and the growing colony of pathogen isolated. Molecular techniques using specific primers were used to identify the V. dahliae pathogens and their mating type of the isolates obtained from the diseased sunflower and potato plants as well as their planting materials. The data obtained revealed that the dominant mating type population in sunflower was MAT1-1, whiles that of potato was MAT1-2, but Race 2 was the only race type identified for all the samples. There was a significant presence of MAT1-1 isolates present in potatoes, which is a new trend. Conventional crop rotation farming using sunflower is causing an increasing prevalence of MAT1-1 and mating type shift of isolates in potato in these regions.

Unraveling verticillium wilt resistance: insight from the integration of transcriptome and metabolome in wild eggplant

“…In recent years, eggplant production in China has become increasingly industrialized, and continuous cropping has led to increasingly severe eggplant wilt disease, causing serious losses in eggplant production ( Pisuttu et al., 2020 ). The research on the resistance mechanism of verticillium wilt has made breakthroughs in crops such as cotton ( Gao et al., 2011 ), tomato ( Chai et al., 2003 ; Fu et al., 2016 ), and potato ( Yan et al., 2022 ), but little is known about the mechanism of resistance to verticillium wilt in eggplant. The rapid development of molecular biology technology has provided a foundation for the in-depth exploration of the mechanism of resistance to wilt disease in eggplant.…”

Identification and Biological Characteristics of Mortierella alpina Associated with Chinese Flowering Cherry (Cerasus serrulata) Leaf Blight in China

“…The TIANGEN Golden Easy PCR kit (TIANGEN Biotech, Beijing, China) was used to carry out the PCR. The PCR amplification reaction was adapted from Liu et al [25] and Yan et al [26]. The PCR products with evident bands, detected with 1% agarose gel electrophoresis, were sent to Tsingke Biotech, Fuzhou, China, for sequencing.…”

Unveiling Cotton's Defense: Harnessing GthZIM17-1 Inhibition for Verticillium Wilt Resistance

“…There six species of the pathogenic fungi however, two are known to be highly virulent, the Verticillium dahliae and Verticillium albo-atrum [9], the others species includes V. tricorpus, V. nigrescens, V. nubilum, and V. theobroma [10]. In China, the main pathogenic fungi is the Verticillium dahliae, V. dahliae is a semi-living nutrient pathogen, that can survive in the form of microsclerotia in the soil for many years, moreover, the microsclerotia are extremely adaptable to extremely harsh environments, and easily dispersed by wind or rain, which makes it di cult to control [11]. The longevity, effective disposal mechanism, and highly adaptive nature to harsh environmental conditions, have made the various control measures to be ineffective, furthermore, there is no effective fungicide that can be used in controlling V. dahliae infection in plants.…”