Identification and Comparison of Bioactive Components of Two Dryopteris sp. Extract Using LC-QTOF-MS

Abstract

Dryopteris sp. is known for its various pharmacological effects and is used as a traditional medicine in Asia. The present study investigated the chemical composition and antimicrobial activity of Dryopteris sp. distributed in Korea. The chemical compounds in the ethanolic extracts of Dryopteris lacera and Dryopteris bissetiana were investigated by ultra-high performance liquid chromatography–quadrupole time-of-flight–mass spectrometry analysis and identified by exploring the UNIFI traditional medicine library. Flavonoids such as juglanin, 6-hydroxyluteolin 7-O-laminaribioside, peltatoside, kaempferitrin, hyperoside, and astragalin were identified in both D. lacera and D. bissetiana. Neochlorogenic acid was identified as a caffeoylquinic acid in D. bissetiana. Both extracts of D. lacera and D. bissetiana exhibited antibacterial activity against Gram-positive pathogens, Staphylococcus aureus and Streptococcus mutans. The minimum inhibitory concentration of D. bissetiana against S. aureus was less than 625 ppm. The antibacterial activity was attributed to the identified phenolic compounds, juglanin, 6-hydroxyluteolin 7-O-laminaribioside, kaempferitrin, astragalin, and neochlorogenic acid. Therefore, D. lacera and D. bissetiana can be used as Gram-positive selective antibiotics for further investigation.

Characterization of Bioactive Metabolites and Antioxidant Activities in Solid and Liquid Fractions of Fresh Duckweed (Wolffia globosa) Subjected to Different Cell Wall Rupture Methods

“…Compounds 30 and 31 had been tentatively identified as astragalin 7-rhamnoside ([M – H] − m / z 593.1486) and (3″-apiosyl-6″-malonyl)astragalin ([M – H] − m / z 725.144) in the CWR, the TWR, the TWF, and the BWF, respectively, in our study. In previous research, this compound was identified in Hibiscus mutabilis L. 67 and Dryopteris sp., 68 which has been proven to be able to inhibit the growth of liver cancer, 69 as well as to inhibit the proliferation of lung cancer. 70 Compounds 32 and 33 were identified in the TWR, TWF, and CWF samples as apigenin 7-glucoside and luteolin 4′- O -glucoside with the precursor ion [M – H] − m / z 431.0968 and [M – H] − m / z 447.0941, respectively.…”