Identification and comparative expression profiles of chemosensory genes in major chemoreception organs of a notorious pests, Laodelphax striatellus

Li, Yao; Hu, Jia; Yin, Xiaoxing; Zhang, Yunye; Chen, Danyu; Liu, Fang

doi:10.1016/j.cbd.2019.100646

Cited by 5 publications

(13 citation statements)

References 54 publications

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“…Mapped genes were manually retrieved using keywords (e.g., E2, ubiquitin, ubiquitin-conjugating enzyme, ubiquitin related enzyme, UBC, ubiquitination) and a BLASTx algorithm-based search, as described previously [ 40 ]. Predicted protein sequences of potential UBC-containing genes were recovered with ORFfinder ( ) and confirmed by BLASTp.…”

Section: Methodsmentioning

confidence: 99%

“…Sequences encoding LstrE2s were initially aligned with ClustalW. Phylogenetic trees were obtained using maximum likelihood analysis in MEGA 5.2 ( ) and analyzed as described previously [ 40 ].…”

Section: Methodsmentioning

confidence: 99%

“…Total RNA was extracted from 50 insect samples [40], and RNA concentration and purity were evaluated as described [41]. cDNA libraries were generated using established methods [40]; these were sequenced with Illumina HiSeq™ 2000 (Illumina, San Diego, CA, USA), and 125-150 bp paired-end reads were generated. Raw reads were filtered and assembled, and clean data were generated and assembled as described [40].…”

Section: Illumina Sequencingmentioning

confidence: 99%

“…cDNA libraries were generated using established methods [40]; these were sequenced with Illumina HiSeq™ 2000 (Illumina, San Diego, CA, USA), and 125-150 bp paired-end reads were generated. Raw reads were filtered and assembled, and clean data were generated and assembled as described [40]. Small brown planthopper genome and annotation files were downloaded directly from the GigaScience repository, GigaDB (RRID: SCR004002), and clean reads were aligned with the reference genome (Bioproject number: PRJNA393384) using HISAT v. 2.…”

Section: Illumina Sequencingmentioning

confidence: 99%

“…Similarly, LstrE2s and LstrActin (control) primers (Table S2) were designed based on LstrE2s and LstrActin sequences, respectively. RT-qPCR was conducted using a CFX96™ Real-Time PCR Detection System using reagents and parameters described previously [40]. Relative expression levels for triplicate samples were calculated using the ∆∆Ct method, and expression levels of target genes were normalized to LstrActin.…”

Section: Real-time Rt-qpcrmentioning

confidence: 99%

See 4 more Smart Citations

Ubiquitin-Conjugating Enzyme E2 E Inhibits the Accumulation of Rice Stripe Virus in Laodelphax striatellus (Fallén)

Shen

et al. 2020

Viruses

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The ubiquitin–proteasome system (UPS) is an essential protagonist in host–pathogen interactions. Among the three classes of enzymes in the UPS, ubiquitin-conjugating enzyme E2 plays a dual role in viral pathogenesis; however, the role of insect E2s in interactions with plant viruses is unclear. Twenty E2-encoding genes in Laodelphax striatellus, the small brown planthopper, were identified and classified into 17 groups by transcriptomic and phylogenetic analysis. Full-length cDNAs of four LstrE2s (LstrE2 A/E/G2/H) were obtained by rapid-amplification of cDNA ends (RACE-PCR) analysis. Expression of the four LstrE2s showed tissue- and development-specific patterns. RT-qPCR analyses revealed that Rice stripe viruse (RSV) infection increased the level of LstrE2 A/E/G2/H. Further study indicated that repression of LstrE2 E via RNAi caused significant increases in the expression of RSV coat protein mRNA and protein levels. These findings suggest that LstrE2 E inhibits RSV accumulation in the planthopper body. Understanding the function of LstrE2 E in RSV accumulation may ultimately result in the development of novel antiviral strategies.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Illumina Sequencingmentioning

confidence: 99%

Section: Illumina Sequencingmentioning

confidence: 99%

Section: Real-time Rt-qpcrmentioning

confidence: 99%

See 3 more Smart Citations

Ubiquitin-Conjugating Enzyme E2 E Inhibits the Accumulation of Rice Stripe Virus in Laodelphax striatellus (Fallén)

Shen

et al. 2020

Viruses

Self Cite

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Identification of Olfactory Genes From the Greater Wax Moth by Antennal Transcriptome Analysis

et al. 2021

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The olfactory system is used by insects to find hosts, mates, and oviposition sites. Insects have different types of olfactory proteins, including odorant-binding proteins (OBPs), chemosensory proteins (CSPs), odorant receptors (ORs), ionotropic receptors (IRs), and sensory neuron membrane proteins (SNMPs) to perceive chemical cues from the environment. The greater wax moth, Galleria mellonella, is an important lepidopteran pest of apiculture. However, the molecular mechanism underlying odorant perception in this species is unclear. In this study, we performed transcriptome sequencing of G. mellonella antennae to identify genes involved in olfaction. A total of 42,544 unigenes were obtained by assembling the transcriptome. Functional classification of these unigenes was determined by searching against the Gene Ontology (GO), eukaryotic orthologous groups (KOG), and the Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. We identified a total of 102 olfactory-related genes: 21 OBPs, 18 CSPs, 43 ORs, 18 IRs, and 2 SNMPs. Results from BLASTX best hit and phylogenetic analyses showed that most of the genes had a close relationship with orthologs from other Lepidoptera species. A large number of OBPs and CSPs were tandemly arrayed in the genomic scaffolds and formed gene clusters. Reverse transcription-quantitative PCR results showed that GmelOBP19 and GmelOR47 are mainly expressed in male antennae. This work provides a transcriptome resource for olfactory genes in G. mellonella, and the findings pave the way for studying the function of these genes.

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Genomics Analysis of Bacillus megaterium 1259 as a Probiotic and Its Effects on Performance in Lactating Dairy Cows

Deng

Wang

et al. 2021

Animals

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In this study, we isolated a novel bacterium, Bacillus megaterium 1259 (BM1259), from chicken manure. Whole-genome sequencing analysis showed that the BM1259 complete genome is composed of a 5,043,095 bp circular chromosome and three circular plasmids, and it encodes 5379 coding genes and 182 RNA genes. Among these genes, a series of nitrate assimilation-related genes and pathways were identified, implying a potential role of BM1259 in nitrate metabolism. In addition, 24 lactating Holstein dairy cows were randomly assigned to four groups that were fed a total mixed ration (TMR) diet only (C), a TMR diet supplemented with 5 g/day of BM1259 (T1), a TMR diet supplemented with 10 g/day of BM1259 (T2), or a TMR diet supplemented with 15 g/day of BM1259 (T3). The results showed that supplementing dairy cows with 15 g/day of BM1259 increased 4% fat-corrected milk production. The molar proportion of propionate (C3) was significantly higher in T2 than in C. The C2:C3 ratio of T3 was higher than those of C and T2. No negative effect of BM1259 on blood indicators was detected. This study demonstrates BM1259 can be applied as a potential probiotic to improve nitrogen utilization and milk production in lactating dairy cows.

show abstract

Identification and comparative expression profiles of chemosensory genes in major chemoreception organs of a notorious pests, Laodelphax striatellus

Cited by 5 publications

References 54 publications

Ubiquitin-Conjugating Enzyme E2 E Inhibits the Accumulation of Rice Stripe Virus in Laodelphax striatellus (Fallén)

Ubiquitin-Conjugating Enzyme E2 E Inhibits the Accumulation of Rice Stripe Virus in Laodelphax striatellus (Fallén)

Identification of Olfactory Genes From the Greater Wax Moth by Antennal Transcriptome Analysis

Genomics Analysis of Bacillus megaterium 1259 as a Probiotic and Its Effects on Performance in Lactating Dairy Cows

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