Identification and comparative expression analysis of chalcone synthase, flavanone 3-hydroxylase and dihydroflavonol 4-reductase genes in wild pomegranate (Punica granatum L.) organs

Kaur, Ramanjeet; Aslam, Lubna; Kapoor, Nisha; Mahajan, Ritu

doi:10.1007/s40415-020-00648-x

Cited by 9 publications

(5 citation statements)

References 74 publications

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“…These genes are closely related to fruit coloration, and the expression pattern increases with fruit development [42]. PgCHS, PgF3H, and PgDFR isolated in pomegranate are highly expressed in red fruits, and their transcript levels show a synchronous increase with the accumulation of flavonoids [43]. C4H, 4CL, and UFGT play a decisive role in anthocyanin production in passionfruit [44].…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic and Metabolomic Analysis of Quality Changes during Sweet Cherry Fruit Development and Mining of Related Genes

Chen

Yang

et al. 2022

IJMS

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Sweet cherries are economically important fruit trees, and their quality changes during development need to be determined. The mechanism of fruit quality changes in sweet cherries were determined by analyzing sweet cherry fruits at 12 developmental stages. The results showed that the soluble sugar, anthocyanin content, and hormones of sweet cherries all changed drastically during the color transition. Therefore, the fruits at the beginning of color conversion, at the end of color conversion, and at the ripening state were selected for the comprehensive analysis of their metabolome and transcriptome. Different sugars, such as D-glucose, sucrose, and trehalose, were identified in the metabolome. Dihydroquercetin, delphinidin-3-glucoside, cyanidin-3-rutincoside, and other flavonoid species were also identified. D-glucose and cyanidin-3-rutinoside were among the most important components of sweet cherry soluble sugars and anthocyanins, respectively. The transcriptional analysis identified key structural genes and nine transcription factors involved in the ABA, sugar, organic acid, and anthocyanin synthesis pathways, with the following specific regulatory patterns. NAC71, WRKY57, and WRKY3 regulate fruit sugar accumulation mainly by acting on INV, SPS, and SUS. MYC2 is involved in the synthesis of anthocyanin precursors by activating PAL and C4H, whereas TCP7 mainly regulates CHI and F3H. WRKY3, NAC71, and WRKY57 have important positive regulatory significance on anthocyanin accumulation, mainly by activating the expression of DFR, ANS, and 3GT.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic and Metabolomic Analysis of Quality Changes during Sweet Cherry Fruit Development and Mining of Related Genes

Chen

Yang

et al. 2022

IJMS

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“…In plants, fungi, and bacteria, chalcone synthase (CHS) is a crucial enzyme for the bio-synthesis of a wide range of compounds [17,18]. In S. marianum, the expression of the CHS 1, 2, and 3 genes has been linked to flavonoid bio-synthesis, including silybin composition [17].…”

Section: Discussionmentioning

confidence: 99%

“…The chalcone synthase enzyme (CHS) family supports plants, fungi, and bacteria to synthesize a variety of secondary metabolites [18,19]. It is an allosteric enzyme that is essential for the synthesis of flavonolignans in S. marianum and other plants [17,20].…”

Section: Introductionmentioning

confidence: 99%

Exogenous Application of Aloe vera Leaf Extract Improves Silybin Content in Silybum marianum L. by Up-Regulating Chalcone Synthase Gene

et al. 2022

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Biotic elicitors such as Aloe vera extract (ALE) have been shown to stimulate growth and modify the bioactive composition of various plant species. ALE has a unique mixture of nutrients that support plant production and growth. In this study, the bio-stimulative effects of ALE foliar spray on plant production and growth, silybin levels, and chalcone synthase gene expression in Silybum marianum were examined. The findings indicated that foliar spray of all the ALE concentrations under study increased plant growth and yield. Additionally, by raising the silybin level of the plant extract, ALE increased the therapeutic value of S. marianum. Further, the activation of the chalcone synthase gene by ALE was analyzed by gene expression research. S. marianum’s growth and production were improved by the application of 60 mL/L ALE, while the silybin level and the chalcone synthase gene expression levels were improved by the application of 40 mL/L ALE. In addition, methanolic fruit extract that contained a higher silybin content also demonstrated a higher anti-microbial activity against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus.

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“…The primers used for qRT-PCR are listed in (Table 1). And, the PgActin gene was used as the reference gene [43,44].…”

Section: Extraction Of Rna and Qrt-pcr Validationsmentioning

confidence: 99%

Effects of 5-Aminolevulinic Acid (5-ALA) on Physicochemical Characteristics and Growth of Pomegranate (Punica granatum L.)

Liu

et al. 2023

Horticulturae

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The pomegranate is one of the most essential crop fruits in the world. 5-Aminolevulinic acid (ALA) regulates the growth and development of 5-year-old pomegranate. However, for plenty of pome crops, conventional information on the physio-chemical characteristics underlying 5-ALA is limited. Therefore, in this study, we applied four different concentrations of 5-ALA treatments (0 mg L−1, 10 mg L−1, 20 mg L−1, 50 mg L−1, and 100 mg L−1), where 0 mg L−1 was considered as a control group, to evaluate the effects of 5-ALA on the physiochemical characteristics of pomegranates. Our results showed that maximal photochemical efficiency (FV/FM), photosystem (ΦPSII), and photochemical quenching (qP) increased at concentrations of 50 mg L−1 and 100 mg L−1 compared with controls. Anthocyanin contents were elevated by 2.27% and 1.33% at the 5-ALA concentrations of 50 mg L−1 and 100 mg L−1. Furthermore, qRT-PCRs of the DEGs, such as punica granatum chalcone synthase (PgCHS), chalcone isomerase (PgCHI), flavanone 3-hydroxylase (PgF3H), dihydroflavonol 4-reductase (PgDFR), anthocyanidin synthase (PgANS), and ultrafine-grained (PgUFG), showed up-regulations, except for chalcone isomerase (PgCHI), after 5-ALA treatments. The fresh weight of the fruit and the weight of the grains were elevated under 50 mg L−1 and 100 mg L−1 concentrations, and both the fresh weight of the fruit and the grain weight were higher than controls. Total sugar (TS) increased by 8.49% and 24.99%, total soluble solids (TSS) increased by 2.02% and 6.07%, soluble proteins increased by 1.16% and 1.15%, and the pH level of juice increased by 0.12% and 0.19%, respectively. In addition, the contents of ascorbic acid, total phenols, and total flavonoids increased by 24.18%, 2.53%, and 1.29%, respectively, compared with controls. Taken together, the antioxidant activities of SOD and POD also increased by 13.33% and 11.95% at concentrations of 100 mg L−1. Our results show that concentrations of 5-ALA treatments at 50 mg L−1 and 100 mg L−1 will be beneficial for crop cultivation.

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Identification and comparative expression analysis of chalcone synthase, flavanone 3-hydroxylase and dihydroflavonol 4-reductase genes in wild pomegranate (Punica granatum L.) organs

Cited by 9 publications

References 74 publications

Transcriptomic and Metabolomic Analysis of Quality Changes during Sweet Cherry Fruit Development and Mining of Related Genes

Transcriptomic and Metabolomic Analysis of Quality Changes during Sweet Cherry Fruit Development and Mining of Related Genes

Exogenous Application of Aloe vera Leaf Extract Improves Silybin Content in Silybum marianum L. by Up-Regulating Chalcone Synthase Gene

Effects of 5-Aminolevulinic Acid (5-ALA) on Physicochemical Characteristics and Growth of Pomegranate (Punica granatum L.)

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