Identification and chromosomal mapping of a third mouse runt-like locus

Calabi, Franco; Rhodes, Michael D.; Williamson, Penny; Boyd, Yvonne

doi:10.1016/0888-7543(95)80184-n

Cited by 15 publications

(6 citation statements)

References 18 publications

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“…In mammals the runtrelated genes are termed RUNX [van Wijnen et al, 2004]. Three human and murine RUNX genes-RUNX1 (AML1, CBFA2 and Pebp2ab), RUNX2 (AML3, CBFA1 and Pebp2aa) and RUNX3 (AML2, CBFA3 and Pebp2ac)-have been identified and localised on human chromosomes 21q22.12, 6p21 and 1p36.1 and mouse chromosomes 16, 17 and 4 respectively [Bae et al, 1994;Levanon et al, 1994;Avraham et al, 1995;Calabi et al, 1995;Otto et al, 1997;Zhang et al, 1997].…”

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confidence: 99%

Control of RUNX2 isoform expression: The role of promoters and enhancers

Stöck

Otto

2005

J. Cell. Biochem.

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The three mammalian RUNX genes constitute the family of runt domain transcription factors that are involved in the regulation of a number of developmental processes such as haematopoiesis, osteogenesis and neuronal differentiation. All three genes show a complex temporo-spatial pattern of expression. Since the three proteins are probably mutually interchangeable with regard to function, most of the specificity of each family member seems to be based on a tightly controlled regulation of expression. While RUNX gene expression is driven by two promoters for each gene, the promoter sequence alone does not seem to suffice for a proper expressional control. This review focuses on the available evidence for the existence of such control mechanisms and studies aiming at discovering cis-acting regulatory sequences of the RUNX2 gene.

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confidence: 99%

Control of RUNX2 isoform expression: The role of promoters and enhancers

Stöck

Otto

2005

J. Cell. Biochem.

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“…The Runt domain is responsible for both sequence-specific binding at the consensus sequence PyGPyG GTPy and the interaction with the ␤ subunit (43,45,57,59,72). CBF␣ subunits are encoded by a family of three known genes, Cbf/␣1 (also called Pebp2␣A and Aml3), Cbf/␣2 (Pebp2␣B, Aml1), and Cbf ␣3 (Pebp2␣C, Aml2) (2,4,11,45,51,63,72,102). In humans, these genes are called CBFA1, CBFA2, and CBFA3, respectively (33).…”

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confidence: 99%

Transcriptional activation of a retrovirus enhancer by CBF (AML1) requires a second factor: evidence for cooperativity with c-Myb

Zaiman

Lenz

1996

J Virol

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Transcriptional enhancer sequences within the long terminal repeats (LTRs) of murine leukemia viruses are the primary genetic determinants of the tissue specificity and potency of the oncogenic potential of these retroviruses. SL3-3 (SL3) is a murine leukemia virus that induces T-cell lymphomas. The LTR enhancer of this virus contains two binding sites for the transcription factor CBF (also called AML1 and PEBP2) that flank binding sites for c-Myb and the Ets family of factors. Using cotransfection assays in P19 cells, we report here that CBF and c-Myb cooperatively stimulate transcription from the SL3 LTR. By itself, c-Myb had no stimulatory effect on transcription. However, when cotransfected with a cDNA encoding one form of the ␣ subunit of CBF called CBF␣2-451, a level of transactivation higher than that seen with CBF␣2-451 alone was detected. The negative regulatory domain near the carboxyl terminus of c-Myb did not affect this activity. Electrophoretic mobility shift assays indicated that CBF and c-Myb bind to DNA independently. Therefore, it appears that the cooperative stimulation of transcription by these factors occurs at a step in the process of transcription after the two factors are bound to the enhancer. Sequences near the carboxyl terminus of CBF␣2-451 were important for cooperativity with c-Myb, consistent with previous reports that this region contains an activation domain. However, CBF␣2-451 failed to activate transcription from a version of the SL3 LTR in which the enhancer was replaced with five tandem CBF-binding sites. Thus, it appears that transcriptional activation of the SL3 enhancer by CBF requires that an appropriate heterologous transcription factor be bound to a neighboring site in the regulatory sequences.

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“…Three highly conserved human and mouse runt domain (RD) genes RUNX1, RUNX2 and RUNX3 (RUNX is used when description applies to both human and mouse, whereas Runx is used when findings involve only the mouse genes) were identified and localized on human chromosomes 21q22.12, 6p21 and 1p36.1 and on mouse chromosomes 16, 17 and 4, respectively (Bae et al, 1994(Bae et al, , 1995Levanon et al, 1994;Avraham et al, 1995;Calabi et al, 1995;Zhang et al, 1997a). The RUNX are key regulators of lineage-specific gene expression in major developmental pathways.…”

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confidence: 99%

Structure and regulated expression of mammalian RUNX genes

2004

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The RUNX are key regulators of lineage-specific gene expression in major developmental pathways. The expression of RUNX genes is tightly regulated, leading to a highly specific spatio/temporal expression pattern and to distinct phenotypes of gene knockouts. This review highlights the extensive structural similarities between the three mammalian RUNX genes and delineates how regulation of their expression at the levels of transcription and translation are orchestrated into the unique RUNX expression pattern.

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Identification and chromosomal mapping of a third mouse runt-like locus

Cited by 15 publications

References 18 publications

Control of RUNX2 isoform expression: The role of promoters and enhancers

Control of RUNX2 isoform expression: The role of promoters and enhancers

Transcriptional activation of a retrovirus enhancer by CBF (AML1) requires a second factor: evidence for cooperativity with c-Myb

Structure and regulated expression of mammalian RUNX genes

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