Identification and characterization of three telomere repeat-binding factors in rice

Byun, Mi Young; Hong, Jong Pil; Kim, Woo Taek

doi:10.1016/j.bbrc.2008.04.181

Cited by 19 publications

(26 citation statements)

References 26 publications

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“…In contrast, all of the GFP‐fused class I proteins displayed a speckled pattern in chloroplasts. As a control for specificity, the localization pattern of rice telomere repeat‐binding factor 1 (OsTRBF1) [16] was determined. As previously observed, OsTRBF1 resides in the nucleus.…”

Section: Resultsmentioning

confidence: 99%

Enzymatic characterization of class I DAD1‐like acylhydrolase members targeted to chloroplast in Arabidopsis

Seo

Kim

et al. 2009

FEBS Letters

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Edited by Ulf-Ingo FlüggeKeywords: DEFECTIVE IN ANTHER DEHISCENCE1-like acylhydrolase Phospholipase Galactolipase Chloroplast targeting Alternative RNA splicing a b s t r a c tIn Arabidopsis, there are at least seven class I acylhydrolase members, which have a putative N-terminal chloroplast-targeting signal. Here, we show that all seven class I proteins are localized to the chloroplasts and hydrolyze phosphatidylcholine at the sn-1 position. However, based on their activities toward various lipids, Arabidopsis class I enzymes could be further divided into three sub-groups by substrate specificity, one with phospholipase-specific activity, another with phospholipase and galactolipase activities, and the other with broad lipolytic activity toward phosphatidylcholine, galactolipids, and triacylglycerol. These results suggest that the three sub-groups of class I acylhydrolases have specific roles in chloroplasts.

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Section: Resultsmentioning

confidence: 99%

Enzymatic characterization of class I DAD1‐like acylhydrolase members targeted to chloroplast in Arabidopsis

Seo

Kim

et al. 2009

FEBS Letters

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“…One possibility is that OsKu70 and RTBP1 function cooperatively to stabilize the structure and function of telomeres in rice. Thus, it would be interesting to test whether OsKu70 interacts with RTBP1 and/or OsTRBF, another class of rice telomere-binding proteins (Byun et al, 2008), in the presence or absence of OsKu80.…”

Section: Discussionmentioning

confidence: 99%

OsKu70 Is Associated with Developmental Growth and Genome Stability in Rice

Hong

Byun

et al. 2009

Plant Physiology

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The cellular functions of Ku70 in repair of DNA double-stranded breaks and telomere regulation have been described in a wide range of organisms. In this study, we identified the rice (Oryza sativa) Ku70 homolog (OsKu70) from the rice genome database. OsKu70 transcript was detected constitutively in every tissue and developmental stage examined and also in undifferentiated callus cells in rice. Yeast two-hybrid and in vitro pull-down experiments revealed that OsKu70 physically interacts with OsKu80. We obtained loss-of-function osku70 T-DNA knockout mutant lines and constructed transgenic rice plants that overexpress the OsKu70 gene in the sense (35S:OsKu70) or antisense (35S:anti-OsKu70) orientation. The homozygous G2 osku70 mutant lines were more sensitive than wild-type plants to a DNA-damaging agent (0.01%-0.05% methyl-methane sulfonate), consistent with the notion that OsKu70 participates in the DNA repair mechanism. Terminal restriction fragment analysis revealed that telomeres in homozygous G2 osku70 mutants were markedly longer (10-20 kb) than those in wild-type plants (5-10 kb), whereas telomere length in heterozygous G2 osku70 mutant and T2 OsKu70-overexpressing transgenic (35S: OsKu70) rice resembled that of the wild-type plant. In contrast to what was observed in Arabidopsis (Arabidopsis thaliana) atku70 mutants, homozygous G2 osku70 rice plants displayed severe developmental defects in both vegetative and reproductive organs under normal growth conditions, resulting in sterile flowers. Analysis of meiotic progression in pollen mother cells demonstrated that up to 11.1% (seven of 63) of G2 mutant anaphase cells displayed one or more chromosomal fusions. These results suggest that OsKu70 is required for the maintenance of chromosome stability and normal developmental growth in rice plants.

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“…In vitro pull‐down and immuno‐blot analyses were performed as described by Byun et al . () with anti‐His and anti‐MBP antibody (Applied Biological Materials, Richmond, BC, Canada).…”

Section: Methodsmentioning

confidence: 99%

Suppression of OsRAD51D results in defects in reproductive development in rice (Oryza sativa L.)

Byun

Kim

2014

The Plant Journal

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SUMMARYThe cellular roles of RAD51 paralogs in somatic and reproductive growth have been extensively described in a wide range of animal systems and, to a lesser extent, in Arabidopsis, a dicot model plant. Here, the Os-RAD51D gene was identified and characterized in rice (Oryza sativa L.), a monocot model crop. In the rice genome, three alternative OsRAD51D mRNA splicing variants, OsRAD51D.1, OsRAD51D.2, and OsRAD51D.3, were predicted. Yeast two-hybrid studies, however, showed that only OsRAD51D.1 interacted with Os-RAD51B and OsRAD51C paralogs, suggesting that OsRAD51D.1 is a functional OsRAD51D protein in rice. Loss-of-function osrad51d mutant rice plants displayed normal vegetative growth. However, the mutant plants were defective in reproductive growth, resulting in sterile flowers. Homozygous osrad51d mutant flowers exhibited impaired development of lemma and palea and contained unusual numbers of stamens and stigmas. During early meiosis, osrad51d pollen mother cells (PMCs) failed to form normal homologous chromosome pairings. In subsequent meiotic progression, mutant PMCs represented fragmented chromosomes. The osrad51d pollen cells contained numerous abnormal micro-nuclei that resulted in malfunctioning pollen. The abnormalities of heterozygous mutant and T2 Ubi:RNAi-OsRAD51D RNAi-knock-down transgenic plants were intermediate between those of wild type and homozygous mutant plants. The osrad51d and Ubi:RNAi-OsRAD51D plants contained longer telomeres compared with wild type plants, indicating that OsRAD51D is a negative factor for telomere lengthening. Overall, these results suggest that OsRAD51D plays a critical role in reproductive growth in rice. This essential function of OsRAD51D is distinct from Arabidopsis, in which AtRAD51D is not an essential factor for meiosis or reproductive development.

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Identification and characterization of three telomere repeat-binding factors in rice

Cited by 19 publications

References 26 publications

Enzymatic characterization of class I DAD1‐like acylhydrolase members targeted to chloroplast in Arabidopsis

Enzymatic characterization of class I DAD1‐like acylhydrolase members targeted to chloroplast in Arabidopsis

OsKu70 Is Associated with Developmental Growth and Genome Stability in Rice

Suppression of OsRAD51D results in defects in reproductive development in rice (Oryza sativa L.)

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