Identification and Characterization of the Bovine Herpesvirus 5 US4 Gene and Gene Products

Engelhardt, Thyl; Keil, Günther M.

doi:10.1006/viro.1996.0580

Cited by 29 publications

(20 citation statements)

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“…The U S region, extending from positions 116733 to 126280, contains eight genes (US1.67, 2 to 4, and 6 to 9), four of which have been previously sequenced (1,20,22,31). BHV-5 U S genes exhibit 69 to 98% amino acid identity (average, 79%) and overall less conservation to BHV-1 homologues than those within the U L region (84% average amino acid identity) (58).…”

Section: Resultsmentioning

confidence: 99%

“…The US2 homologue in HSV-1 and HSV-2 seems dispensable in tissue culture and is not involved in HSV-2 neuropathogenesis in mice (52,61). BHV-5 US4 encodes a glycosaminoglycan-associated protein (31) and is 72% identical to BHV-1 gG, which is involved in cell-cell virus transmission in vitro (69) and in prevention of apoptosis in certain cell lines (68). BHV-5 US8 encodes gE, which is important for neurovirulence in rabbits.…”

Section: Resultsmentioning

confidence: 99%

“…The complete BHV-1 genomic sequence, a composite including sequences of five different virus strains, is available (GenBank accession number AJ004801). However, less than 15% of the BHV-5 genome (mostly representing envelope glycoproteins) has been sequenced (1,18,20,22,31,40,64,80,86). Here we present the complete sequence of a neurovirulent strain of BHV-5, with analysis and comparison to BHV-1.…”

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confidence: 99%

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Genome of Bovine Herpesvirus 5

Delhon

Moraes

et al. 2003

J Virol

143

114

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Here we present the complete genomic sequence of bovine herpesvirus 5 (BHV-5), an alphaherpesvirus responsible for fatal meningoencephalitis in cattle. The 138,390-bp genome encodes 70 putative proteins and resembles the ␣2 subgroup of herpesviruses in genomic organization and gene content. BHV-5 is very similar to BHV-1, the etiological agent of infectious bovine rhinotracheitis, as reflected by the high level of amino acid identity in their protein repertoires (average, 82%). The highest similarity to BHV-1 products (>95% amino acid identity) is found in proteins involved in viral DNA replication and processing (UL5, UL15, UL29, and UL39) and in virion proteins (UL14, UL19, UL48, and US6). Among the least conserved (<75%) are the homologues of immediate-early (IE) proteins BICP0, BICP4, and BICP22, the three proteins being longer in BHV-5 than in BHV-1. The structure of the BHV-5 latency-related (LR) region departs markedly from that of BHV-1 in both coding and transcriptional regulatory regions. Given the potential significance of IE genes and the LR region in virus-neuron interactions, it is likely these differences contribute to BHV-5 neuropathogenicity.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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confidence: 99%

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Genome of Bovine Herpesvirus 5

Delhon

Moraes

et al. 2003

J Virol

143

114

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“…In addition, secreted gG can be found as protein species ranging from 90 to 240 kDa when linked to GAGs (27,33). BHV-1 gG is nonessential for viral growth but essential for cell-to-cell spread in bovine kidney cells (47,58).…”

Section: Bhv-1 and Bhv-5 Ggmentioning

confidence: 99%

Alphaherpesviruses and Chemokines: Pas de Deux Not Yet Brought to Perfection

Walle

Jarosinski

Osterrieder

2008

J Virol

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“…1C). The upstream fragment that was chosen was the 1.8 kb NruI fragment that starts 149 nt upstream of the end of the gG coding region, covers the complete gD coding region and ends at the start of the gI coding region (5,8). The downstream fragment that was chosen was 1.6 kb NotI fragment that starts at the end of the US9 coding region, covers the partial US1.67 gene and ends in the terminal repeat region (5).…”

Section: Construction Of a Gi/ge/us9 Deletion Fragmentmentioning

confidence: 99%

Construction and characterization of a bovine herpesvirus 5 mutant with a deletion of the gI, gE and US9 genes

Franco

Hübner

Oliveira³

et al. 2007

Braz. J. Microbiol.

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Bovine herpesvirus 5 (BoHV-5) is a important cause of viral encephalitis in cattle in South America. Within the framework of developing a differential vaccine against BoHV-5, a deletion mutant was constructed based on a Brazilian BoHV-5 isolate. The target of the deletions were genes that code proteins implicated in the neurovirulence of BoHV-5, the glycoprotein I (gI), glycoprotein E (gE) and membrane protein US9. To construct the deletion mutant of BoHV-5, the flanking regions of all three genes were cloned in a prokaryotic plasmid. This deletion fragment was co-transfected with the viral DNA into bovine cells. Identification of deletion mutants was performed by immunostaining with an anti-gE monoclonal antibody. One of the gE negative viral populations found was purified, amplified and further examined by restriction endonuclesase analysis of its genomic DNA. The plaque sizes and penetration kinetics of the deletion mutant and wild type viruses were compared. The plaque sizes of the deletion mutant were significantly smaller than those of the parental strain (p ≤ 0.05), but no statistical differences were observed in penetration kinetics. The results indicate that the gI/ gE/US9 deletion mutant of BoHV-5 may have a reduced virulence in the host and is still viable enough to be a good candidate for the development of a BoHV-5 vaccine.

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Identification and Characterization of the Bovine Herpesvirus 5 US4 Gene and Gene Products

Cited by 29 publications

References 0 publications

Genome of Bovine Herpesvirus 5

Genome of Bovine Herpesvirus 5

Alphaherpesviruses and Chemokines: Pas de Deux Not Yet Brought to Perfection

Construction and characterization of a bovine herpesvirus 5 mutant with a deletion of the gI, gE and US9 genes

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