Identification and characterization of the nifH and nifH promoter regions located on the nif-plasmid pEA3 of Enterobacter agglomerons 333

Kreutzer, Roland; Singh, Mahavir; Klingmüller, Walter

doi:10.1016/0378-1119(89)90318-1

Cited by 21 publications

(8 citation statements)

References 28 publications

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“…Preparation of total bacterial DNA: Total DNA of the bacterial strains used was prepared based on the methods of KREUTZER et al (1989) andGLIESCHE et al (1997). The total DNA was resuspended in 300 µl TE buffer and 9 µl 10 mg/ml RNase was added and incubated for 1.5 hours at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

Identification of nif genes in N₂‐fixing bacterial strains isolated from rice fields along the Yangtze River Plain

Xie

Cui

et al. 2006

J. Basic Microbiol.

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The aim of this research was to identify nifH and nifHDKYE ' genes in twenty strains of N2-fixing heterotrophic bacteria isolated from rice fields in the Yangtze River Plain. Southern hybridization of the total DNA from each strain was performed with the Klebsiella pneumoniae nifHDKYE ' gene probe (6.2 kb Eco RI fragment from pSA30) and the Azospirillum brasilense nifH gene probe (0.6 kb Eco RI-Hin dIII fragment from pHU8). We found that Eco RI fragments of total DNA from Aeromonas hydrophila HY2, Bacillus azotoformans FD, Bacillus licheniformis NCH1, NCH5, WH4, Bacillus brevis NC2, Bacillus pumilus NC12, Bacillus cereus NCH2, Citrobacter freundii HY5, HY9, Derxia gummosa HZ5, Pseudomonas mendocina HZ1 and Pseudomonas pseudoalcaligenes WH3 were positively hybridized with both of the probes. Agrobacterium radiobacter HY17, Corynebacterium sp. HY12, YZ and Pseudomonas sp. HY11 had Eco RI fragments hybridized with the K. pneumoniae nifHDKYE ' gene probe. An Eco RI fragment of total DNA from Bacillus megaterium YY4 was positively hybridized to the A. brasilense nifH gene probe. No hybridization sign was found in the total DNA fragments from Alcaligenes cupidus YY6 and Corynebacterium sp. NC11 hybridized with either of the gene probes. The data provide the number and size of EcoRI fragments of the total DNA hybridized with the nif gene probes for these strains of rarely studied species, suggesting additional evidence for N2 fixing and nif gene diversity of N2-fixing bacteria in rice fields along the Yangtze River Plain.

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Section: Methodsmentioning

confidence: 99%

Identification of nif genes in N₂‐fixing bacterial strains isolated from rice fields along the Yangtze River Plain

Xie

Cui

et al. 2006

J. Basic Microbiol.

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“…Partial sequences have also been published for nifJ from Enterobacter agglomerans 333 (Kreutzer et al, 1989) and Anabaena PCC7119 (Schmitz et al, 1993) that are also highly similar to the R. rubrum sequence.…”

Section: The Deduced Amino Acid Sequencementioning

confidence: 99%

**Identification and sequence of a nifJ‐like gene in Rhodospirillum rubrum: partial characterization of a mutant unaffected in nitrogen fixation**

Lindblad

Jansson

Brostedt

et al. 1996

Molecular Microbiology

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A nifJ-like gene was identified in the photosynthetic purple non-sulphur bacterium Rhodospirillum rubrum. A DNA segment hybridizing to Klebsiella pneumoniae nifJ was isolated, the gene was inactivated, and a mutant strain, SNJ-1, was constructed by allele replacement. The mutation was confirmed by DNA sequencing. Northern blotting and by the lack of pyruvate oxidoreductase activity. This is the first report of a nifJ-like gene in photosynthetic bacteria. Unexpectedly, SNJ-1 was capable of nitrogen fixation, and growth was similar to the wild-type strain under all conditions investigated. Therefore, this is also the first demonstration that a nifJ homologue, when present, is not essential for nitrogen fixation in a diazotroph. The nifJ-like gene was sequenced and found to have considerable similarity to published nifJ gene sequences from other organisms. By primer extension, the initiation site for transcription was located, and a typical sigma 54 promoter sequence was identified.

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“…Sequencing of the Enterobacter agglomerans nifU promoter For sequencing, a 435bp PstI-Sau3a fragment of the Enterobacter agglomerans nifU-lacZ' fusion plasmid pMK182P8 (23) was cloned into pUC 18 (24). Both strands were sequenced on double stranded template DNA using dideoxynucleotide termination sequencing methods.…”

Section: Assays Of Promoter Activitymentioning

confidence: 99%

Activation of theKlebsiella pneumoniae nifUpromoter: identification of multiple and overlapping upstream NifA binding sites

Cannon¹,

Kreutzer²,

Kent³

et al. 1990

Nucl Acids Res

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The Klebsiella pneumoniae nifU promoter is positively controlled by the NifA protein and requires a form of RNA polymerase holoenzyme containing the rpoN encoded sigma factor, sigma 54. Occupancy of the K. pneumoniae nifU promoter by NifA was examined using in vivo dimethyl sulphate footprinting. Three binding sites for NifA (Upstream Activator Sequences, UASs 1, 2 and 3) located at -125, -116 and -72 were identified which conform to the UAS consensus sequence TGT-N10-ACA. An additional NifA binding site was identified at position -90. The UASs located at -125 (UAS1) and -116 (UAS2) overlap and do not appear to bind NifA as independent sites. They may represent a NifA binding site interacting with two NifA dimers. UAS3 is located at -72, and abuts a binding site for integration host factor (IHF) and is not normally highly occupied by NifA. In the absence of IHF UAS3 showed increased occupancy by NifA. Mutational and footprinting analysis of the three UASs indicates (1) IHF and NifA can compete for binding and that this competition influences the level of expression from the nifU promoter (2) that UAS2 is a principle sequence of the UAS 1,2 region required for activation and (3) that none of the NifA binding sites interacts with NifA independently. In vivo KMnO4 footprinting demonstrated that NifA catalyses open complex formation at the nifU promoter. IHF was required for maximal expression from the nifU and nifH promoters in Escherichia coli, and for the establishment of a Nif+ phenotype in E. coli from the nif plasmid pRD1.

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Identification and characterization of the nifH and nifH promoter regions located on the nif-plasmid pEA3 of Enterobacter agglomerons 333

Cited by 21 publications

References 28 publications

Identification of nif genes in N₂‐fixing bacterial strains isolated from rice fields along the Yangtze River Plain

Identification of nif genes in N₂‐fixing bacterial strains isolated from rice fields along the Yangtze River Plain

**Identification and sequence of a nifJ‐like gene in Rhodospirillum rubrum: partial characterization of a mutant unaffected in nitrogen fixation**

Activation of theKlebsiella pneumoniae nifUpromoter: identification of multiple and overlapping upstream NifA binding sites

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Identification and characterization of the nifH and nifH promoter regions located on the nif-plasmid pEA3 of Enterobacter agglomerons 333

Cited by 21 publications

References 28 publications

Identification of nif genes in N2‐fixing bacterial strains isolated from rice fields along the Yangtze River Plain

Identification of nif genes in N2‐fixing bacterial strains isolated from rice fields along the Yangtze River Plain

Identification and sequence of a nifJ‐like gene in Rhodospirillum rubrum: partial characterization of a mutant unaffected in nitrogen fixation

Activation of theKlebsiella pneumoniae nifUpromoter: identification of multiple and overlapping upstream NifA binding sites

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Identification of nif genes in N₂‐fixing bacterial strains isolated from rice fields along the Yangtze River Plain

Identification of nif genes in N₂‐fixing bacterial strains isolated from rice fields along the Yangtze River Plain

**Identification and sequence of a nifJ‐like gene in Rhodospirillum rubrum: partial characterization of a mutant unaffected in nitrogen fixation**