Identification and Characterization of the<i>OCT4</i>Upstream Regulatory Region in<i>Sus scrofa</i>

Kim, Seung‐Hun; Choi, Kwang‐Hwan; Lee, Dong‐Kyung; Lee, Mingyun; Hwang, Jae Yeon; Lee, Chang Kyu

doi:10.1155/2019/2130973

Cited by 6 publications

(14 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the two conditions were similar to embryonic carcinoma cells than embryonic stem cells in mouse. In a previous luciferase analysis experiment in mouse, the porcine OCT4 distal enhancer was highly activated in the naïve state on E14, and the proximal enhancer was highly activated in the primed state on P19 [29]. However, the proximal enhancer in the porcine embryonic broblast reprogramming process was more activated than the distal enhancer in both reprogramming culture conditions (Fig.…”

Section: Distal and Proximal Enhancer Activity Patterns In Lif-or Fgf-dependent Dox Reprogramming Media Culture Conditionmentioning

confidence: 68%

“…Nucleotide sequences from the porcine OCT4 upstream region were more similar to the human OCT4 upstream region rather than counterpart of mouse Oct4. In all conserved regions and a 3.2-kb upstream region, total nucleotide, including distal and proximal enhancers, homology between human and pig is higher than between mouse and pig [29]. Enhancers are key contributors to gene expression patterns via various mechanisms [14].…”

Section: Discussionmentioning

confidence: 99%

“…Porcine OCT4 upstream region-derived dual reporter system Porcine-speci c OCT4 upstream region-derived dual reporter systems were produced as described previously [29]. The following two vectors were used primarily in this study: pOCT4-∆PE-eGFP vector (DE-GFP) containing Conserved regions (CR) 1 and 4, and a pOCT4-∆DE-DsRed2 vector (PE-RFP) containing CR1, 2, and 3.…”

Section: Methodsmentioning

confidence: 99%

“…However, a large difference was found in the Oct4 upstream region nucleotide sequence between species, and the differences in expression occurred when the Oct4 upstream region-based reporter systems constructed from one species were inserted into another species. Porcine proximal enhancer-based vectors did not work properly in mouse pluripotent stem cells [29]. Therefore, a porcine-speci c OCT4 reporter system, which is important for porcine pluripotency research, required functional evaluation in porcine origin pluripotent cells.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Porcine OCT4 Reporter System Can Monitor Species-Specific Pluripotency During Somatic Cell Reprogramming

Kim

Choi

Lee

et al. 2021

Cellular Reprogramming

Self Cite

View full text Add to dashboard Cite

l Background: The present study examined the activity and function of pig OCT4 enhancer in porcine reprogramming cells. Dual uorescent protein reporter systems controlled by the upstream regulatory region of OCT4, which is one of the master regulators for pluripotency, are widely used in studies of the mechanism of pluripotency. We analyzed how this reporter system functions in FGF-or LIF-dependent reprogrammed porcine pluripotent stem cells using the previously established porcine-speci c reporter system.l Results: Porcine embryonic broblasts were coinfected with the pOCT4-∆PE-eGFP (DE-GFP) and pOCT4-∆DE-DsRed2 (PE-RFP) vectors, and GFP and RFP expression was veri ed during a DOX-dependent reprogramming process. We demonstrated that the porcine OCT4 distal enhancer and proximal enhancer were activated in different expression patterns simultaneously as the changes in the expression of pluripotent marker genes during the establishment of porcine-induced pluripotent stem cells (iPSCs). l Conclusions: Porcine OCT4 upstream region-derived dual uorescent protein reporter systems serve as live naïve/primed pluripotency indicators for porcine induced pluripotent cell establishment. This work demonstrates the applicability of the porcine OCT4 upstream region-derived dual uorescence reporter system, which may be applied to investigations of species-speci c pluripotency in porcine-origin cells.These reporter systems may be useful tools for studies of porcine-speci c pluripotency, early embryo development and embryonic stem cells.

show abstract

Section: Distal and Proximal Enhancer Activity Patterns In Lif-or Fgf-dependent Dox Reprogramming Media Culture Conditionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Porcine OCT4 Reporter System Can Monitor Species-Specific Pluripotency During Somatic Cell Reprogramming

Kim

Choi

Lee

et al. 2021

Cellular Reprogramming

Self Cite

View full text Add to dashboard Cite

show abstract

“…They can be duplicated over 250 times and have long telomers [13,33]. Also, it is important to consider that OCT4 upstream region is important in pluripotency in early embryo development and the establishment of embryonic stem cells [34].…”

Section: Msc Characteristics and Sourcesmentioning

confidence: 99%

Different approaches for transformation of mesenchymal stem cells into hepatocyte-like cells

et al. 2020

View full text Add to dashboard Cite

Due to the prominent role of the liver in the body and detoxification, its functionality can be affected in an irreversible manner by diseases. This phenomenon renders the liver to stop working, leading to morbidity and mortality. Therefore, liver transplantation is the only way to tackle this issue. In order to compensate for the lack of adequate healthy liver tissue for transplantation, therapeutic approaches such as hepatocyte transplantation have been proposed as an alternative. Recognizing the fact that mesenchymal stem cells are adult stem cells with the capacity to differentiate into several cell types, different methods have been invented to produce hepatocyte-like cells from mesenchymal stem cells. They can be divided into three main categories, such as addition of cytokines and growth factors, genetic modifications, and adjustment of microenvironment as well as physical parameters. In this review, we attempted to introduce diverse efficient methods for differentiating mesenchymal stem cells and their capability for transformation into hepatocyte-like cells.

show abstract

Changes in OCT4 expression play a crucial role in the lineage specification and proliferation of preimplantation porcine blastocysts

Lee

Choe

et al. 2022

Cell Proliferation

Self Cite

View full text Add to dashboard Cite

Objectives: Curiosity about the role of OCT4, a core transcription factor that maintains inner cell mass (ICM) formation during preimplantation embryogenesis and the pluripotent state in embryonic development, has long been an issue. OCT4 has a speciesspecific expression pattern in mammalian preimplantation embryogenesis and is known to play an essential role in ICM formation. However, there is a need to study new roles for OCT4-related pluripotency networks and second-cell fate decisions. Materials and Methods:To determine the functions of OCT4 in lineage specification and embryo proliferation, loss-and gain-of-function studies were performed on porcine parthenotes using microinjection. Then, we performed immunocytochemistry and quantitative real-time polymerase chain reaction (PCR) to examine the association of OCT4 with other lineage markers and its effect on downstream genes.Results: In OCT4-targeted late blastocysts, SOX2, NANOG, and SOX17 positive cells were decreased, and the total cell number of blastocysts was also decreased. According to real-time PCR analysis, NANOG, SOX17, and CDK4 were decreased in OCT4-targeted blastocysts, but trophoblast-related genes were increased. In OCT4-overexpressing blastocysts, SOX2 and NANOG positive cells increased, while SOX17 positive cells decreased, and while total cell number of blastocysts increased. As a result of real-time PCR analysis, the expression of SOX2, NANOG, and CDK4 was increased, but the expression of SOX17 was decreased. Conclusion:Taken together, our results demonstrated that OCT4 leads pluripotency in porcine blastocysts and also plays an important role in ICM formation, secondary cell fate decision, and cell proliferation. | INTRODUCTIONMammalian embryos undergo two cell fate decisions during preimplantation embryogenesis. 1 The first decision is divided into an inner cell mass and trophectoderm, 2 and the second decision segregates ICM into epiblast and primitive endoderm. 3,4 Numerous genes and mechanisms are involved in this lineage specification, and OCT4 has been heavily studied due to it's importance. OCT4 is one of the core transcription factors of pluripotency, has a DNAbinding domain, and controls expression by binding to a consensus

show abstract

Identification and Characterization of theOCT4Upstream Regulatory Region inSus scrofa

Cited by 6 publications

References 38 publications

Porcine OCT4 Reporter System Can Monitor Species-Specific Pluripotency During Somatic Cell Reprogramming

Porcine OCT4 Reporter System Can Monitor Species-Specific Pluripotency During Somatic Cell Reprogramming

Different approaches for transformation of mesenchymal stem cells into hepatocyte-like cells

Changes in OCT4 expression play a crucial role in the lineage specification and proliferation of preimplantation porcine blastocysts

Contact Info

Product

Resources

About