Identification and characterization of the telomerase reverse transcriptase of Bombyx mori (silkworm) and Tribolium castaneum (flour beetle)

Osanai, Mizuko; Kojima, Kenji; Futahashi, Ryo; Yaguchi, Shigeo; Fujiwara, Haruhiko

doi:10.1016/j.gene.2006.04.022

Cited by 69 publications

(64 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…9 When telomerase activity is low, telomere length is also maintained by a recombination-mediated mechanism, 10,11 or, in some species, by both insertion of telomeric retrotransposons into telomerase-synthesized repeats and recombination. 12 In these scenarios, it is important to notice that canonical telomeric repeats remain at the ends.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Telomere maintenance in Drosophila: Rapid transposon evolution at chromosome ends

Villasanté

Pablos²,

Méndez-Lago³

et al. 2008

Cell Cycle

View full text Add to dashboard Cite

The maintenance of terminal sequences is an important role of the telomere, since it prevents the loss of internal regions that encode essential genes. In most eukaryotes, this is accomplished by the telomerase. However, telomere length can also be maintained by other mechanisms, such as homologous recombination and transposition of telomeric retrotransposons to the chromosome ends. A remarkable situation is the case of Drosophila, where telomerase was lost, and thus telomeres managed to be maintained by occasional retrotransposition of telomeric elements to the receding ends. In the recent analysis of 12 Drosophila genomes, the multiplicity of autonomous and non-autonomous telomere-specific retrotransposons has revealed extensive and rapid evolution of telomeric DNA. The phylogenetic relationship among these telomeric retrotransposons is congruent with the species phylogeny, suggesting that they have been vertically transmitted from a common ancestor. In this review, we also suggest that the formation of a non-canonical DNA structure at Drosophila telomeres could be the way to protect the ends.

show abstract

Section: Introductionmentioning

confidence: 99%

“…In agreement with this hypothesis, no telomerase gene has been identified in the genomes of Anopheles gambiae and Drosophila melanogaster. 12 Equally, neither an orthologue of the G overhang-binding proteins nor one of the telomeric repeat-binding proteins have been found in these genomes.…”

Section: Introductionmentioning

confidence: 99%

Telomere maintenance in Drosophila: Rapid transposon evolution at chromosome ends

Villasanté

Pablos²,

Méndez-Lago³

et al. 2008

Cell Cycle

View full text Add to dashboard Cite

show abstract

“…Tribolium has a telomerase and telomeres containing TCAGG repeats 13 , a variant of the standard arthropod TTAGG telomeric repeat. Manual assembly of the proximal regions of multiple telomeres beyond the ends of the assembled scaffolds (Supplementary Information) reveals TCAGG repeats interrupted by full-length and 59-truncated non-LTR retrotransposons belonging to the R1 clade, best known for insertions in the rDNA locus 14 .…”

mentioning

confidence: 99%

The genome of the model beetle and pest Tribolium castaneum

2008

Nature

1,235

694

View full text Add to dashboard Cite

Tribolium castaneum is a member of the most species-rich eukaryotic order, a powerful model organism for the study of generalized insect development, and an important pest of stored agricultural products. We describe its genome sequence here. This omnivorous beetle has evolved the ability to interact with a diverse chemical environment, as shown by large expansions in odorant and gustatory receptors, as well as P450 and other detoxification enzymes. Development in Tribolium is more representative of other insects than is Drosophila, a fact reflected in gene content and function. For example, Tribolium has retained more ancestral genes involved in cell-cell communication than Drosophila, some being expressed in the growth zone crucial for axial elongation in short-germ development. Systemic RNA interference in T. castaneum functions differently from that in Caenorhabditis elegans, but nevertheless offers similar power for the elucidation of gene function and identification of targets for selective insect control.

show abstract

“…As a corollary, identification of gene encoding TERT in P. monodon (PmTERT) is of great importance as the envisaged immortalization of shrimp cells could be possible with the ectopic expression of PmTERT. The TERT cDNA has been isolated and characterized from yeasts ) and vertebrates such as fish (Rao et al 2011), frog (Kuramoto et al 2001), chicken (Delany and Daniels 2004), mouse (Greenberg et al 1998), hamster , dog (Nasir et al 2004) and human ; invertebrates such as ascidians (Li et al 2007), protozoan (Bryan et al 1998), and insects (Gillis et al 2008;Osanai et al 2006). However, the telomerase reverse transcriptase has not yet been identified from P. monodon.…”

Section: Discussionmentioning

confidence: 99%

Impaired telomerase activity hinders proliferation and in vitro transformation of Penaeus monodon lymphoid cells

et al. 2015

View full text Add to dashboard Cite

Retaining terminal transferase activity of telomerase, the ribonucleoprotein enzyme which add telomeric repeats on chromosome end is thought to be required to prevent cellular ageing. Additionally, telomerase considered as a marker for cell proliferation and immortalization in eukaryotes. We examined telomerase activity in tissues and lymphoid cell culture of Penaeus monodon. Along with telomerase activity, telomere repeats and an attempt on identification of telomerase reverse transcriptase (PmTERT) were made. Telomeric repeat amplification protocol revealed that telomerase-dependent telomeric lengthening has been taking place in P. monodon and the adult tissues were retaining this capacity throughout their lifespan with the highest activity in ovary, testis and lymphoid organ. However, telomerase activity could not be detected in lymphoid cells in culture. The canonical telomeric repeats added by telomerase of lymphoid tissue extract were identified as TTAGG, but pentameric repeats GGTTA and AGGTT were also added by the telomerase. PmTERT protein sequence (partial) shared 100 % identity with the TERT sequence of Daphnia pulex, 27 % sequence identity with Purple sea urchin and 24-25 % with Zebra fish. Undetectable telomerase activity in lymphoid cell culture supports the hypothesis that the inadequate telomerase activity or gene expression may be a reason that prevents neoplastic transformation and spontaneous immortalization of the cells in vitro. Thus, it is envisaged that telomerase activation in lymphoid cells may surmount cellular ageing for in vitro transformation and cell line establishment.

show abstract

Identification and characterization of the telomerase reverse transcriptase of Bombyx mori (silkworm) and Tribolium castaneum (flour beetle)

Cited by 69 publications

References 40 publications

Telomere maintenance in Drosophila: Rapid transposon evolution at chromosome ends

Telomere maintenance in Drosophila: Rapid transposon evolution at chromosome ends

The genome of the model beetle and pest Tribolium castaneum

Impaired telomerase activity hinders proliferation and in vitro transformation of Penaeus monodon lymphoid cells

Contact Info

Product

Resources

About