Identification and Characterization of the Pheromones in the Minute Pirate Bug Orius sauteri (Heteroptera: Anthocoridae)

Uehara, Takuya; Maeda, Taro; Shimoda, Masami; Fujiwara-Tsujii, Nao; Yasui, Hiroe

doi:10.1007/s10886-019-01104-1

Cited by 9 publications

(3 citation statements)

References 41 publications

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“…However, as living organisms, these insects may not always exhibit the targeted effectiveness of chemical pesticides. We have developed tools to address this issue thus far (Ogino et al, 2016; Tokushima et al, 2016; Uehara et al, 2019a,b). With the advent of genome editing technology, enhancement of the efficacy of natural enemies through breeding has become increasingly feasible.…”

Section: Resultsmentioning

confidence: 99%

High-quality genome of the zoophytophagous stink bug,Nesidiocoris tenuis, informs their food habit adaptation

Shibata,

Shimoda,

Kobayashi

et al. 2023

Preprint

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The zoophytophagous stink bug,Nesidiocoris tenuis, is a promising natural enemy of micro-pests such as whiteflies and thrips. This bug possesses both phytophagous and entomophagous food habits, enabling it to obtain nutrition from both plants and insects. This trait allows us to maintain its population density in agricultural fields by introducing insectary plants, even when the pest prey density is extremely low. However, if the bugs' population becomes too dense, they can sometimes damage crop plants. This dual character seems to arise from the food preferences and chemosensation of this predator. To understand the genomic landscape ofN. tenuis, we examined the whole genome sequence of a commercially available Japanese strain. We used long-read sequencing and Hi-C analysis to assemble the genome at the chromosomal level. We then conducted a comparative analysis of the genome with previously reported genomes of phytophagous and hematophagous stink bugs to focus on the genetic factors contributing to this species' herbivorous and carnivorous tendencies. Our findings suggest that the gustatory gene set plays a pivotal role in adapting to food habits, making it a promising target for selective breeding. Furthermore, we identified the whole genomes of microorganisms symbiotic with this species through genomic analysis. We believe that our results shed light on the food habit adaptations ofN. tenuisand will accelerate breeding efforts based on new breeding techniques for natural enemy insects, including genomics and genome editing.

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Section: Resultsmentioning

confidence: 99%

High-quality genome of the zoophytophagous stink bug,Nesidiocoris tenuis, informs their food habit adaptation

Shibata,

Shimoda,

Kobayashi

et al. 2023

Preprint

Self Cite

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“…Studies found that they take advantage of kairomone emanating from their prey [60,61] to benefit their offspring. Moreover, we know that O. insidiosus and O. sauteri exhibit a pronounced sexual dimorphism of the metathoracic scent gland secretion [62,63]. Females of O. insidiosus produce a volatile sex pheromone and a non-volatile trail pheromone.…”

Section: Discussionmentioning

confidence: 99%

Influence of Plant Physical and Anatomical Characteristics on the Ovipositional Preference of Orius sauteri (Hemiptera: Anthocoridae)

Zhang

Qin

et al. 2021

Insects

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Natural enemies play an important role in managing insect pests. Orius sauteri (Poppius) (Hemiptera: Anthocoridae), a predator of many soft-body insects, is an important biological control agent in Asia. Understanding this predator’s egg-laying preferences and a habitat needs is important for its success in pest control. We investigated the plant acceptability and ovipositional preference of O. sauteri for coriander (Coriadrum sativum L., Apiales: Apiaceae), marigold (Tagetes erecta L., Asterales: Asteraceae), sweet alyssum (Lobularia maritima L., Brassicales: Brassicaceae), and alfalfa (Medicago sativa L., Fabales: Fabaceae), and focused on the effects of plant physical and anatomical characteristics on the ovipositional preference of O. sauteri. The results showed that O. sauteri can lay eggs on uninfested plants in the vegetative stage and their eggs hatched normally. Orius sauteri females prefer plants with high stomatal density, a large stomatal area, and fewer trichomes as oviposition hosts, and the depth of egg placement was determined by leaf thickness. Our studies suggested that O. sauteri females can select oviposition hosts and specific oviposition sites by assessing the structural qualities of plant surface. Coriander and marigold are potentially suitable host plants for O.sauteri. The results aid the selection of cover crops to enhance natural enemies in the fields.

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“…(Hemiptera: Anthopteridae) originated in Asia but are now distributed worldwide. As an important euryphagous predator, they are widely used to control small pests, such as aphids, thrips, mites, and some lepidopterans, on crops in fields and greenhouses [1][2][3][4][5]. In Asia, it is an important biological control agent for agricultural and forestry insect pests [6].…”

Section: Introductionmentioning

confidence: 99%

Effect of Guanylate Cyclase-22-like on Ovarian Development of Orius nagaii (Hemiptera: Anthocoridae)

Du,

Wang,

Dai

et al. 2024

Insects

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This study identified and characterized the gene encoding recep tor-type guanylate cyclase-22-like (GCY-22; OnGCY) from the pirate bug Orius nagaii, an important biological control agent. The full-length cDNA of the GCY of O. nagaii was obtained by rapid amplification of cDNA ends (RACE); it had a total length of 4888 base pairs (bp), of which the open reading frame (ORF) was 3750 bp, encoding a polypeptide of 1249 amino acid residues. The physicochemical properties of OnGCY were predicted and analyzed by using relevant ExPASy software, revealing a molecular formula of C6502H10122N1698O1869S57, molecular weight of ~143,811.57 kDa, isoelectric point of 6.55, and fat index of 90.04. The resulting protein was also shown to have a signal peptide, two transmembrane regions, and a conserved tyrosine kinase (tyrkc). Silencing OnGCY by RNA interference significantly inhibited ovarian development and decreased fertility in female O. nagaii in the treated versus the control group. Additionally, OnGCY silencing significantly decreased the expression levels of other GCY and Vg genes. Thus, these results clarify the structure and biological function of OnGCY, which has an important role in insect fecundity. The results also provide a reference for agricultural pest control and future large-scale breeding of biological control agents.

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Identification and Characterization of the Pheromones in the Minute Pirate Bug Orius sauteri (Heteroptera: Anthocoridae)

Cited by 9 publications

References 41 publications

High-quality genome of the zoophytophagous stink bug,Nesidiocoris tenuis, informs their food habit adaptation

High-quality genome of the zoophytophagous stink bug,Nesidiocoris tenuis, informs their food habit adaptation

Influence of Plant Physical and Anatomical Characteristics on the Ovipositional Preference of Orius sauteri (Hemiptera: Anthocoridae)

Effect of Guanylate Cyclase-22-like on Ovarian Development of Orius nagaii (Hemiptera: Anthocoridae)

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