Identification and characterization of the novel leaf rust resistance gene Lr81 in wheat

Xu, Xiangyang; Kolmer, J. A.; Li, Genqiao; Tan, Chibing; Carver, Brett F.; Bian, Ruolin; Bernardo, Amy; Bai, Guihua

doi:10.1007/s00122-022-04145-5

Cited by 14 publications

(13 citation statements)

References 35 publications

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“…The chromosome 2AS-located Lr17a gene was widely presented in wheat varieties adapted to North America and its resistance has been overcome by newly merged Pt pathotypes [28]. Seedling resistance gene Lr81 was identified in a Croatian breeding line "PI 470121" and mapped to an approximately 100 Kb genomic region on chromosome 2AS using Kompetitive allele-specific PCR (KASP) markers [29]. A novel leaf rust resistance gene LrM introgressed from Aegilops markgrafii provides high degree of resistance against multiple Pt pathotypes.…”

Section: Lr/qlr On Chromosome 2amentioning

confidence: 99%

Genetics of Resistance to Leaf Rust in Wheat: An Overview in A Genome-Wide Level

Ren¹,

Wang²,

Ren³

et al. 2022

Preprint

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Due to the global warming and dynamic changes in pathogenic virulence, leaf rust caused by Puccinia triticina has greatly expanded its epidermic region and become a severe threat to global wheat production. Genetic bases of wheat resistance to leaf rust mainly relies on the leaf rust resistance (Lr) gene or quantitative trait locus (QLr). Although these genetic loci have been insensitively studied during the last two decades, an updated overview of Lr/QLr in a genome-wide level is urgently needed. This review summarized recent progresses in genetic studies of wheat resistance to leaf rust. Wheat germplasms with great potentials in genetic improvement of resistance to leaf rust were highlighted. Key information about the genetic loci carrying Lr/QLr were summarized. A genome-wide chromosome distribution map for all the Lr/QLr was generated based on the released wheat reference genome. In conclusion, this review has provided valuable sources for both wheat breeders and researchers to understand the genetics of resistance to leaf rust in wheat.

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Section: Lr/qlr On Chromosome 2amentioning

confidence: 99%

Genetics of Resistance to Leaf Rust in Wheat: An Overview in A Genome-Wide Level

Ren¹,

Wang²,

Ren³

et al. 2022

Preprint

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“…However, wheat production worldwide is threatened by leaf rust caused by Puccinia triticina (Pt), which is the most destructive and widespread rust in wheat growing regions, causing yield losses higher than 1% globally (Savary et al 2019) and even up to 70% for susceptible cultivars (Aktar-Uz-Zaman et al 2017). The most e cient and eco-friendly method to reduce the threat of wheat leaf rust is developing resistant cultivars (Xu et al 2022).…”

Section: Introductionmentioning

confidence: 99%

“…To date, 108 genes conferring resistance to wheat leaf rust have been formally catalogued (Dinh et al 2020;Rothwell et al 2020;Kumar et al 2021;Xu et al 2022). Almost half of these leaf rust resistance (Lr) genes are derived from wild relatives (Prasad et al 2020).…”

Section: Introductionmentioning

confidence: 99%

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Genetic mapping of the wheat leaf rust resistance gene Lr19 and development of translocation lines to break the linkage with PSY-E1

Lyu

Zhang

et al. 2023

Preprint

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The Lr19 locus, which was transferred to wheat from the wheat relative Thinopyrum ponticum in 1966, still confers broad resistance to most known leaf rust isolates worldwide. However, this gene has not been fine mapped to day, and its tight linkage with the yellow pigment gene PSY-E1 has limited the deployment of Lr19 in bread wheat breeding. In this paper, we genetically mapped Lr19 using a bi-parental population obtained by crossing two wheat-Th. ponticum substitution lines, 7E1(7D) (carrying Lr19) and 7E2(7D) (susceptible to leaf rust). Genetic analysis of the F2 population and F2:3 families showed that Lr19 is a single dominant gene, and it was successfully mapped within a 0.3 cM interval on the long arm of the Th. ponticum chromosome 7E1, flanked by markers XsdauK3734 and XsdauK2839. To reduce the size of the Th. ponticum chromosome segment carrying Lr19, the Chinese Spring Ph1b mutant was employed to promote recombination between the homoeologous chromosomes of the wheat chromosome 7D and 7E1. Two translocation lines with short Th. ponticum chromosome fragments carrying Lr19 were identified using markers closely linked to Lr19, and in one of the lines the linkage between Lr19 and PSY-E1 was broken. Both translocation lines were resistant to 16 Pt isolates collected from China. Thus, the Lr19 linkage markers and translocation lines developed in this study can be used for marker-assisted selection in common wheat breeding programs.

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“…Due to its high crossability with hexaploid wheat, several resistance genes have been incorporated into wheat ( Li et al, 2019a ). Up to now, a total of 81 leaf rust ( Xu et al, 2022 ) and 83 stripe rust resistance genes ( Li et al, 2020 ) have been officially named in wheat, respectively, but only Lr38 ( Friebe et al, 1993 ) and Yr50 ( Liu et al, 2013 ) were reported from Th. intermedium .…”

Section: Introductionmentioning

confidence: 99%

Molecular cytogenetic characterization of a new wheat-Thinopyrum intermedium homoeologous group-6 chromosome disomic substitution line with resistance to leaf rust and stripe rust

Zhang

Li²,

Ge³

et al. 2022

Front. Plant Sci.

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Thinopyrum intermedium (JJJsJsStSt, 2n = 6x = 42), a member of tertiary gene pool of hexaploid wheat (Triticum aestivum L., AABBDD, 2n = 6x = 42), provides several beneficial genes for wheat improvement. In this study, line CH51 was developed from the BC1F8 progeny of a partial wheat-Th. intermedium amphiploid TAI8335 (2n = 56) and wheat cultivar (cv.) Jintai 170. Somatic metaphase chromosome counting showed that CH51 had stable 42 chromosomes. Genomic in situ hybridization (GISH) analysis showed that CH51 had 40 wheat chromosomes and two Th. intermedium chromosomes involving translocation between Js- and St-genome chromosomes. Non-denaturing fluorescence in situ hybridization (ND-FISH) analysis revealed that CH51 lacked a pair of wheat chromosome 6B. Wheat 55K SNP array analysis verified that chromosome 6B had the highest percentage of missing SNP loci in both CH51 and Chinese Spring (CS) nullisomic 6B-tetrasomic 6D (CS-N6BT6D) and had the highest percentage of polymorphic SNP loci between CH51 and cv. Jintai 170. We identified that CH51 was a wheat-Th. intermedium T6StS.6JsL (6B) disomic substitution line. Disease resistance assessment showed that CH51 exhibited high levels of resistance to the prevalent Chinese leaf rust and stripe rust races in the field. Therefore, the newly developed line CH51 can be utilized as a potential germplasm in wheat disease resistance breeding.

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Identification and characterization of the novel leaf rust resistance gene Lr81 in wheat

Cited by 14 publications

References 35 publications

Genetics of Resistance to Leaf Rust in Wheat: An Overview in A Genome-Wide Level

Genetics of Resistance to Leaf Rust in Wheat: An Overview in A Genome-Wide Level

Genetic mapping of the wheat leaf rust resistance gene Lr19 and development of translocation lines to break the linkage with PSY-E1

Molecular cytogenetic characterization of a new wheat-Thinopyrum intermedium homoeologous group-6 chromosome disomic substitution line with resistance to leaf rust and stripe rust

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