Identification and characterization of the protein product of gene 67 in equine herpesvirus type 1 strain Ab4

Sun, Yi; Maclean, Alasdair; Brown, Stuart A.

doi:10.1099/0022-1317-76-3-541

Cited by 3 publications

(6 citation statements)

References 12 publications

(16 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another indication of a close evolutionary relationship of CHV to EHV-1 is the presence ofa homologue to gene 67, which encodes a 36 kDa virion protein (Sun et al, 1995). Gene 67 homologues are not found in PRV, VZV or HSV-1 (Telford et al, 1992).…”

Section: Organization Of Conserved Genesmentioning

confidence: 99%

Gene organization in the UL region and inverted repeats of the canine herpesvirus genome

Remond

Sheldrick

Lebreton

et al. 1996

Journal of General Virology

View full text Add to dashboard Cite

Restriction mapping and the determination of scattered nucleotide sequences have permitted a description of the global structure and evolutionary affinities of the canine herpesvirus (CHV) genome. The global structure closely resembles that of the totally sequenced genomes of varicella-zoster virus and equine herpesvirus 1 (EHV-1) in having a 37 bp inverted repeat flanking a long unique region (UL) of approximately 100000bp, and a 10100-10 700 bp inverted repeat flanking a short unique region (Us) of roughly 7400-8600 bp. On the basis of the sequences obtained, 35 homologues to previously identifled herpesvirus gene products were found in U L and the major inverted repeat, and the level of the similarities indicated that CHV belongs to the genus Varicellovirus. Within the genus, CHV appears to be most closely related to EHV-1, pseudorabies virus and feline herpesvirus. Surprisingly, genes for both subunits of the viral ribonucleotide reductase were found to be missing from their equivalent place in other herpesvirus genomes. Either they have been translocated to another position in the CHV genome or, we think more likely, they have been lost.

show abstract

Section: Organization Of Conserved Genesmentioning

confidence: 99%

Gene organization in the UL region and inverted repeats of the canine herpesvirus genome

Remond

Sheldrick

Lebreton

et al. 1996

Journal of General Virology

View full text Add to dashboard Cite

show abstract

“…2A). Both fluorescences were associated with thread-like structures that are typical of those formed by the wild-type IR6 protein late in infection (17,19,20,29). When the IR6-specific and laminspecific fluorescences were superimposed, the staining patterns of both the RacL11 IR6 and the lamin proteins were identical as demonstrated by the yellow appearance of the structure ( Fig.…”

Section: Resultsmentioning

confidence: 81%

“…The EHV-1 IR6 protein colocalizes with nuclear lamins. The appearance of the EHV-1 IR6 protein-specific rod-like structures in infected cells suggested a colocalization with proteins that influence the cell's architecture (17,19,20,29). To test the hypothesis that the IR6 protein colocalizes with lamin proteins which are associated with the inner nuclear membrane and are involved in cell division (6), two different approaches were taken.…”

Section: Resultsmentioning

confidence: 99%

“…Of the more than 76 proteins encoded by EHV-1, most share extensive homology with the prototype member of the virus subfamily, herpes simplex virus type 1 (HSV-1) (30). Among the open reading frames (ORFs) that are not present in HSV-1, the IR6 gene (gene 67) and gene product have been identified (2,17,29). Structural homologs of the EHV-1 IR6 protein have been described on the basis of nucleotide sequence analyses in equine herpesvirus 4 (EHV-4), bovine herpesvirus 1 (BHV-1), and canine herpesvirus (CHV) (11,14,26,31).…”

mentioning

confidence: 99%

“…The observed aggregation of the IR6 protein to the rod-like structures led to the hypothesis that it could interact with cellular proteins that form the cytoskeleton (17). However, no association of the IR6 protein with the investigated proteins actin, tubulin, vimentin, dynein, kinesin, and desmin could be shown (17,19,29). To date, the nuclear lamins which represent members of the intermediate filament family have not been analyzed for a putative aggregation with the IR6 protein, although they are expressed in all eukaryotic cells.…”

mentioning

confidence: 99%

See 2 more Smart Citations

The Equine Herpesvirus 1 IR6 Protein That Colocalizes with Nuclear Lamins Is Involved in Nucleocapsid Egress and Migrates from Cell to Cell Independently of Virus Infection

Osterrieder

Neubauer

Brandmüller

et al. 1998

J Virol

View full text Add to dashboard Cite

The equine herpesvirus 1 (EHV-1) IR6 protein forms typical rod-like structures in infected cells, influences virus growth at elevated temperatures, and determines the virulence of EHV-1 Rac strains (Osterrieder et al., Virology 226:243–251, 1996). Experiments to further elucidate the functions and properties of the IR6 protein were conducted. It was shown that the IR6 protein of wild-type RacL11 virus colocalizes with nuclear lamins very late in infection as demonstrated by confocal laser scan microscopy and coimmunoprecipitation experiments. In contrast, the mutated IR6 protein encoded by the RacM24 strain did not colocalize with the lamin proteins at any time postinfection (p.i.). Electron microscopical examinations of ultrathin sections were performed on cells infected at 37 and 40°C, the latter being a temperature at which the IR6-negative RacH virus and the RacM24 virus are greatly impaired in virus replication. These analyses revealed that nucleocapsid formation is efficient at 40°C irrespective of the virus strain. However, whereas cytoplasmic virus particles were readily observed at 16 h p.i. in cells infected with the wild-type EHV-1 RacL11 or an IR6-recombinant RacH virus (HIR6-1) at 40°C, virtually no capsid translocation to the cytoplasm was obvious in RacH- or RacM24-infected cells at the elevated temperature, demonstrating that the IR6 protein is involved in nucleocapsid egress. Transient transfection assays using RacL11 or RacM24 IR6 plasmid DNA and COS7 or Rk13 cells, infection studies using a gB-negative RacL11 mutant (L11ΔgB) which is deficient in direct cell-to-cell spread, and studies using lysates of IR6-transfected cells demonstrated that the wild-type IR6 protein is transported from cell to cell in the absence of virus infection and can enter cells by a yet unknown mechanism.

show abstract

An Equine Herpesvirus-1 Gene 71 Deletant Is Attenuated and Elicits a Protective Immune Response in Mice

Kr¹,

Sun²,

et al. 1997

Virology

View full text Add to dashboard Cite

The pathogenesis of pulmonary infection and the immune response following intranasal inoculation of mice with two equine herpesvirus type 1 (EHV-1) deletion mutants have been assessed. The mutants, ED71 and ED75, have deletions in genes 71 (EUS4) and 75 (10K), respectively. Deletions were replaced by the Escherichia coli lacZ gene driven by the simian virus 40 (SV40) early promoter. It has previously been shown that the protein products of genes 71 and 75 are dispensable in vitro but that removal of gene 71 results in a defect in virus maturation and capsid envelopment which impairs the ability of mutant virus to spread via release and readsorption. This study demonstrated that the 192-kDa gene 71 product is required for full expression of virulence in mice, whereas the putative 10-kDa product of gene 75 has minimal effect. Both mutants exhibited the same tissue and cytotropism as wild-type EHV-1 and induced both humoral and cell-mediated immune responses indistinguishable from those induced by the parental strain. Irrespective of the reduced pathogenicity of the gene 71 mutant, infected mice were protected against a challenge with wild-type EHV-1. These findings highlight the potential of ED71 as a vaccine candidate.

show abstract

Identification and characterization of the protein product of gene 67 in equine herpesvirus type 1 strain Ab4

Cited by 3 publications

References 12 publications

Gene organization in the UL region and inverted repeats of the canine herpesvirus genome

Gene organization in the UL region and inverted repeats of the canine herpesvirus genome

The Equine Herpesvirus 1 IR6 Protein That Colocalizes with Nuclear Lamins Is Involved in Nucleocapsid Egress and Migrates from Cell to Cell Independently of Virus Infection

An Equine Herpesvirus-1 Gene 71 Deletant Is Attenuated and Elicits a Protective Immune Response in Mice

Contact Info

Product

Resources

About