Identification and characterization of telocytes in rat testis

Liu, Yifei; Wang, Siyi; Tarique, Imran; Vistro, Waseem Ali; Zhang, Haiyan; Haseeb, Abdul; Gandahi, Noor Samad; Iqbal, Adeela; An, Tianci; Yang, Huan; Chen, Qiusheng; Yang, Ping

doi:10.18632/aging.102158

Cited by 28 publications

(33 citation statements)

References 36 publications

(48 reference statements)

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“…In this study, we demonstrated the presence of TCs within the connective tissue in between the lobules and gland cells in the pancreas of Chinese soft-shelled turtles by TEM, IHC, and IF, according to the TC diagnostic criteria. α-SMA+ immunoreactivity has been shown in the peritubular myoid cells (smooth muscle-like cells) in the wall of seminiferous tubules, while CD34+ staining is reported in podoms of telocytes in seminiferous tubules of rat testis [66]. Some studies claim that some of the CD34+ TCs can also be positive for α-SMA+, serving as the source cells for differentiation into fibroblasts and myofibroblasts.…”

Section: Discussionmentioning

confidence: 99%

Identification of Telocytes in the Pancreas of Turtles—A role in Cellular Communication

Gandahi

Ding

Shi

et al. 2020

IJMS

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The existence of telocytes (TCs) has not yet been established in the pancreases of aquatic reptiles. Here, we report TCs in the exocrine pancreas of Pelodiscus sinensis using transmission electron microscope (TEM), immunohistochemistry (IHC), and immunofluorescence (IF) techniques. TCs surrounded the acini and ducts of the connective tissue of the exocrine pancreas and between lobules and gland cells. The cells were located preferably close to the blood vessels, interlobular ducts, and nerve fibers. Ultrastructurally, TCs exhibited small and large bodies with thick and thin portions, podoms, and podomers, and prolongations that form dichotomous branching with hetero-cellular and homo-cellular junctions. The podom (thick) portions showed caveolae, mitochondria, rough endoplasmic reticulum, and vesicles. The nucleus carries heterochromatin and is irregular in shape. The shape of TCs depends on the number of telopodes (Tps) bearing long, short, spindle, triangular, and “beads on a string” shapes with twisted, tortuous prolongations and ramifications. Shed extracellular vesicles and exosomes were found frequently released from projections and Tps within connective tissue in the vicinity of the acini and collagen fibers. IHC and IF results showed CD34+, α-SMA+, and vimentin+, long and triangle-shaped TCs, consistent with the TEM findings. The presence of shaded vesicles from TCs might implicate their possible role in immune surveillance, tissue regeneration as well as regulatory functions in the reptilian pancreas.

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Section: Discussionmentioning

confidence: 99%

Identification of Telocytes in the Pancreas of Turtles—A role in Cellular Communication

Gandahi

Ding

Shi

et al. 2020

IJMS

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“…Telocytes are interstitial cells of stromal origin with an oval shape, and a heterochromatic nucleus with moniliform projections denominated telopodes, which characterize its morphological classification according to the number of projections 9 , 10 . They are founded in the interstitium of tissues such as the testicle 9 , smooth and cardiac muscle 11 , human tongue 10 , pancreas 12 , and liver 13 . It can be identified by the electronic microscopy, immunohistochemistry, and CD34+/immunostaining 14 .…”

Section: Introductionmentioning

confidence: 99%

Myotendinous junction adaptations to ladder-based resistance training: identification of a new telocyte niche

Neto

Rocha

Barbosa

et al. 2020

Sci Rep

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The present study shows chronic adjustments in the myotendinous junction (MTJ) in response to different ladder-based resistance training (LRT) protocols. Thirty adult male Wistar rats were divided into groups: sedentary (S), calisthenics (LRT without additional load [C]), and resistance-trained (LRT with extra weight [R]). We demonstrated longer lengths of sarcoplasmatic invaginations in the trained groups; however, evaginations were seen mainly in group R. We showed a greater thickness of sarcoplasmatic invaginations in groups C and R, in addition to greater evaginations in R. We also observed thinner basal lamina in trained groups. The support collagen layer (SCL) adjacent to the MTJ and the diameters of the transverse fibrils were larger in R. We also discovered a niche of telocytes in the MTJ with electron micrographs of the plantar muscle and with immunostaining with CD34+ in the gastrocnemius muscle near the blood vessels and pericytes. We concluded that the continuous adjustments in the MTJ ultrastructure were the result of tissue plasticity induced by LRT, which is causally related to muscle hypertrophy and, consequently, to the remodeling of the contact interface. Also, we reveal the existence of a collagen layer adjacent to MTJ and discover a new micro anatomic location of telocytes. The myotendinous junction (MTJ) consists of a highly specific anatomical region in which the sarcoplasmatic membranes connect to bundles of extracellular matrix (ECM) collagen fibers 1. Due to its functionality, MTJ represents the region with the highest transmission of force 2,3. Morphologically, MTJ presents projections that penetrate the muscle tissue, parallel and directed to the myofibrils, forming sarcoplasmatic invaginations. Currently, we know that its development is mainly influenced by the interactions between myoblasts and ECM elements 4,5. The basal lamina of muscle fiber creates a supramolecular connection structure composed of different levels of proteins from the laminin and collagen polymers located in the ECM 6. This arrangement contributes structurally to the transmission of force, adapting, remodeling, and consequently affecting the macroscopic tendon structure to different stimuli 7,8. Initially, telocytes were described as interstitial Cajal cells 9. Telocytes are interstitial cells of stromal origin with an oval shape, and a heterochromatic nucleus with moniliform projections denominated telopodes, which characterize its morphological classification according to the number of projections 9,10. They are founded in the interstitium of tissues such as the testicle 9 , smooth and cardiac muscle 11 , human tongue 10 , pancreas 12 , and liver 13. It can be identified by the electronic microscopy, immunohistochemistry, and CD34+/immunostaining 14. Recently, telocytes have been found in proximity to and engaging in possible interactions with satellite cells 10. The telocytes projections have terminals (pods) with mitochondria that form junctions with adjacent cells, and they have caveolae that perform exocytos...

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“…The mammalian testis is divided into two compartments, the seminiferous tubules including germ cells in various development stages (spermatogonia, spermatocytes, spermatids, spermatozoa) supported by Sertoli cells and the interstitial tissue consisting of loose connective tissue, blood and lymphatic vessels, Leydig cells, fibroblasts, macrophages, leukocytes, and telocytes [1,2]. Testis physiological function consists of spermatogenesis, a process leading to gametes formation occuring in seminiferous tubules regulated by autocrine/paracrine factors, and steroidogenesis that occurs in Leydig cells [3].…”

Section: Introductionmentioning

confidence: 99%

Role of GPER-Mediated Signaling in Testicular Functions and Tumorigenesis

Chimento

Luca

Nocito

et al. 2020

Cells

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Estrogen signaling plays important roles in testicular functions and tumorigenesis. Fifteen years ago, it was discovered that a member of the G protein-coupled receptor family, GPR30, which binds also with high affinity to estradiol and is responsible, in part, for the rapid non-genomic actions of estrogens. GPR30, renamed as GPER, was detected in several tissues including germ cells (spermatogonia, spermatocytes, spermatids) and somatic cells (Sertoli and Leydig cells). In our previous review published in 2014, we summarized studies that evidenced a role of GPER signaling in mediating estrogen action during spermatogenesis and testis development. In addition, we evidenced that GPER seems to be involved in modulating estrogen-dependent testicular cancer cell growth; however, the effects on cell survival and proliferation depend on specific cell type. In this review, we update the knowledge obtained in the last years on GPER roles in regulating physiological functions of testicular cells and its involvement in neoplastic transformation of both germ and somatic cells. In particular, we will focus our attention on crosstalk among GPER signaling, classical estrogen receptors and other nuclear receptors involved in testis physiology regulation.

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Identification and characterization of telocytes in rat testis

Cited by 28 publications

References 36 publications

Identification of Telocytes in the Pancreas of Turtles—A role in Cellular Communication

Identification of Telocytes in the Pancreas of Turtles—A role in Cellular Communication

Myotendinous junction adaptations to ladder-based resistance training: identification of a new telocyte niche

Role of GPER-Mediated Signaling in Testicular Functions and Tumorigenesis

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