Identification and characterization of starvation induced msdgc-1 promoter involved in the c-di-GMP turnover

Bharati, Binod K.; Swetha, R.; Chatterji, Dipankar

doi:10.1016/j.gene.2013.07.043

Cited by 22 publications

(23 citation statements)

References 35 publications

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“…M. smegmatis has only one copy of the gene encoding the enzyme involved in c-di-GMP turnover. We had noticed previously that the level of c-di-GMP appeared to be low in M. smegmatis (Bharati et al, 2012(Bharati et al, , 2013. The reported cellular concentration in other bacteria is~1-10 mM (Hengge, 2009).…”

Section: Dual-active Dcpa and C-di-gmp Turnovermentioning

confidence: 91%

Characterization of a dual-active enzyme, DcpA, involved in cyclic diguanosine monophosphate turnover in Mycobacterium smegmatis

et al. 2014

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We have reported previously that the long-term survival of Mycobacterium smegmatis is facilitated by a dual-active enzyme MSDGC-1 (renamed DcpA), which controls the cellular turnover of cyclic diguanosine monophosphate (c-di-GMP). Most mycobacterial species possess at least a single copy of a DcpA orthologue that is highly conserved in terms of sequence similarity and domain architecture. Here, we show that DcpA exists in monomeric and dimeric forms. The dimerization of DcpA is due to non-covalent interactions between two protomers that are arranged in a parallel orientation. The dimer shows both synthesis and hydrolysis activities, whereas the monomer shows only hydrolysis activity. In addition, we have shown that DcpA is associated with the cytoplasmic membrane and exhibits heterogeneous cellular localization with a predominance at the cell poles. Finally, we have also shown that DcpA is involved in the change in cell length and colony morphology of M. smegmatis. Taken together, our study provides additional evidence about the role of the bifunctional protein involved in c-di-GMP signalling in M. smegmatis.

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Section: Dual-active Dcpa and C-di-gmp Turnovermentioning

confidence: 91%

Characterization of a dual-active enzyme, DcpA, involved in cyclic diguanosine monophosphate turnover in Mycobacterium smegmatis

et al. 2014

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“…Surface-associated bacteria usually harbor more c-di-GMP regulators than free-living bacteria, presumably as an adaptive strategy (120). O 2 , H 2 O 2 , NO, redox potential, light, sucrose, amino acids, polyamines (such as norspermidine and spermidine), Zn 2ϩ , bile acids, bicarbonate, indole, QS autoinducers, cis-2-dodecenoic acid and cis-11-methyl-dodecenoic acid (unsaturated fatty acids that serve as bacterial diffusible signal factors), and nutritional conditions that cause starvation (or depletion of a specific carbon source such as glucose or glycerol) have been identified as environmental cues that induce the bacterial response via altering the intracellular c-di-GMP concentration (480,(492)(493)(494)(495)(496)(497)(498)(499)(500)(501)(502)(503)(504)(505)(506)(507)(508). However, the vast majority of the environmental signals that modulate the activity of the DGCs and PDEs remain unidentified.…”

Section: Centralized Regulation By Second Messengersmentioning

confidence: 99%

Microbial Surface Colonization and Biofilm Development in Marine Environments

Dang

Lovell

2016

Microbiol Mol Biol Rev

829

636

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SUMMARYBiotic and abiotic surfaces in marine waters are rapidly colonized by microorganisms. Surface colonization and subsequent biofilm formation and development provide numerous advantages to these organisms and support critical ecological and biogeochemical functions in the changing marine environment. Microbial surface association also contributes to deleterious effects such as biofouling, biocorrosion, and the persistence and transmission of harmful or pathogenic microorganisms and their genetic determinants. The processes and mechanisms of colonization as well as key players among the surface-associated microbiota have been studied for several decades. Accumulating evidence indicates that specific cell-surface, cell-cell, and interpopulation interactions shape the composition, structure, spatiotemporal dynamics, and functions of surface-associated microbial communities. Several key microbial processes and mechanisms, including (i) surface, population, and community sensing and signaling, (ii) intraspecies and interspecies communication and interaction, and (iii) the regulatory balance between cooperation and competition, have been identified as critical for the microbial surface association lifestyle. In this review, recent progress in the study of marine microbial surface colonization and biofilm development is synthesized and discussed. Major gaps in our knowledge remain. We pose questions for targeted investigation of surface-specific community-level microbial features, answers to which would advance our understanding of surface-associated microbial community ecology and the biogeochemical functions of these communities at levels from molecular mechanistic details through systems biological integration.

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“…Identification of sigma factors was carried out essentially as described by Bharati et al (2013). In brief, a 350 bp fragment encompassing the promoter region of Rv0494 was PCR amplified using ARpEJ494for and biotin-labelled reverse primer AR494pulldownbiotin.…”

Section: Methodsmentioning

confidence: 99%

“…Such a type of differential expression pattern requires the use of multiple sigma factors. In order to identify the sigma factors initiating the transcription of Rv0494, we followed the approach reported by Bharati et al (2013). Briefly, biotin-labelled reverse primer was used to amplify the 350 bp fragment containing the promoter elements.…”

Section: Rv0494 Shows Enhanced Expression During Nutrient Starvationmentioning

confidence: 99%

Rv0494 is a starvation-inducible, auto-regulatory FadR-like regulator from Mycobacterium tuberculosis

et al. 2015

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Fatty acid metabolism plays an important role in the survival and pathogenesis of Mycobacterium tuberculosis. Lipids are assumed to be the major source of energy during dormancy. Here, we report the characterization of a starvation-inducible, lipid-responsive transcriptional regulator, Rv0494, divergently transcribed from the Rv0493c probable operon. The striking difference in the transcriptional regulatory apparatus between mycobacteria and other well-studied organisms, such as Escherichia coli, is the organization of mycobacterial promoters. Mycobacterial promoters have diverse architectures and most of these promoters function inefficiently in E. coli. In this study, we characterized the promoter elements of Rv0494 along with the sigma factors required for transcription initiation. Rv0494 promoter activity increased under nutrient starvation conditions and was transcribed via two promoters: the promoter proximal to the translational start site was active under standard growth conditions, whilst both promoters contributed to the increased activity seen during starvation, with the major contribution from the distal promoter. Furthermore, Rv0494 translation initiated at a codon located 9 bp downstream of the annotated start codon. Rv0494 bound to its upstream sequence to auto-regulate its own expression; this binding was responsive to long-chain fatty acyl-CoA molecules. We further report Rv0494-mediated transcriptional regulation of the Rv2326c gene -a probable transmembrane ATP-binding transporter encoding gene.

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Identification and characterization of starvation induced msdgc-1 promoter involved in the c-di-GMP turnover

Cited by 22 publications

References 35 publications

Characterization of a dual-active enzyme, DcpA, involved in cyclic diguanosine monophosphate turnover in Mycobacterium smegmatis

Characterization of a dual-active enzyme, DcpA, involved in cyclic diguanosine monophosphate turnover in Mycobacterium smegmatis

Microbial Surface Colonization and Biofilm Development in Marine Environments

Rv0494 is a starvation-inducible, auto-regulatory FadR-like regulator from Mycobacterium tuberculosis

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