Identification and Characterization of Soft Rot Bacterial Pathogens on Phalaenopsis Orchid in Bali

Suputra, I Putu Wirya; Wirya, Gusti Ngurah Alit Susanta; Sari, Nyoman Bintang Kartika; Temaja, I Gede Rai Maya; Innosensia, Ni Luh Putu Citra

doi:10.24198/cropsaver.v5i1.39284

Cited by 2 publications

(2 citation statements)

References 8 publications

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“…Salah satu penyakit yang menimbulkan masalah serius dan mengakibatkan turunnya produksi tanaman anggrek adalah penyakit busuk lunak (Wu et al 2011;Suputra et al 2022). Penyakit ini disebabkan oleh infeksi bakteri patogen yang menyerang bagian jaringan tanaman anggrek, antara lain dari genus Pectobacterium, Pseudomonas, Dickeya, Burkholderia, dan Erwinia.…”

Section: Pendahuluanunclassified

Potensi dan Keragaman Bakteri Endofit sebagai Agens Pemacu Pertumbuhan dan Biokontrol Anggrek

Amba Linggi,

Joko,

Widada

2023

J. Ilmu. Pertan. Indones.

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Soft rot disease in orchid plants is a factor in decreasing orchid production. One way of biological control that has the potential to spur growth is to utilize endophytic bacteria. This study aims to describe the diversity of orchid endophytic bacteria that have the potential as growth promoters and biocontrol agents. Observation parameters include indole acetic acid (IAA) production, inorganic phosphate dissolution, ammonia production of orchid endophytic bacteria, and antagonism of endophytic bacteria to pathogenic bacteria that cause soft rot in vitro. The diversity of orchid endophytic bacteria was analyzed using rep-PCR with BOX-A1R primer. The results showed that 10 isolates of orchid endophytic bacteria were able to produce IAA with a concentration of 79–321 μg/mL, able to dissolve phosphate, and produce ammonia. In antagonism testing against soft rot-causing bacteria, all isolates were able to form an inhibitory zone with a diameter of 3–7.2 cm. Furthermore, diversity analysis using rep-PCR showed that isolates of endophytic bacteria DnPh5, BgCt2, BgVt10, DnLp7, DnBl1, and DnAr4 had a uniform DNA band pattern so that they were grouped together. Meanwhile, isolates of TbPh7, IbtPhm1, DnDr2, and AkOc1 showed different DNA band patterns, indicating that the four isolates were different species or subspecies of bacteria. In dendrogram analysis, DnPh5, BgCt2, BgVt10, DnLp7, DnBl1, and DnAr4 isolates have a similarity of 100%. The similarity of isolates IbtPhm1, DnDr2, and AkOc1 was 90%, 79%, and 68%, respectively, against the other six isolates. TbPh7 isolate is an isolate with a low level of similarity to other isolates, which is about 51%. Keywords: orchid, endophytic bacteria, growth promoter, biocontrol agent, rep-PCR

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Section: Pendahuluanunclassified

Potensi dan Keragaman Bakteri Endofit sebagai Agens Pemacu Pertumbuhan dan Biokontrol Anggrek

Amba Linggi,

Joko,

Widada

2023

J. Ilmu. Pertan. Indones.

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“…This soft rot disease causes huge losses in orchid cultivation, including orchid species, namely Phalaenopsis amabilis (Fu & Huang 2011;Sudarsono et al, 2018), in which Phalaenopsis has a high value in orchid commodities (Griesbach, 2002). The incidence of this pathogenic disease is 12-28% in Phalaenopsis orchids in Bali (Suputra et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Inoculation Methods to Determine Resistance of Phalaenopsis amabilis (L.) Regeneratded from Irradiated Protocorms to Dickeya dadantii

Putri,

Sukma,

Sudarsono

et al. 2024

Planta Tropika

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Soft-rot disease (SRD) in Phalaenopsis, caused Dickeya dadantii, has resulted in significant losses in the orchid sector in Indonesia. This study aimed to evaluate the inoculation method of Dickeya dadantii and identify the resistance response of individual regenerated plantlets of Phal. amabilis from irradiated protocorm. A detached leaf assay was used to evaluate the inoculation method and resistance response of SRD. Based on the results of this study, Dickeya dadantii bacteria could only infect the leaves through wounding tissue. The density of bacteria that could infect leaf tissue was OD600 = 0.2. All dilution factors tested caused soft rot symptoms in P. amabilis. On the other hand, Vanilla planifolia only showed symptoms at a dilution factor of 10-0. Four accessions of regenerated plantlets from irradiated protocorms were resistant to SRD. They were from irradiation 5 Gy (IP 05 Gy-23, IP 05 Gy-31, and IP 05 Gy-33) and one accession from the control treatment or without irradiation (IP 0 Gy -1). These results showed that 5 Gy irradiation increased plant resistance to SRD in Phalaenopsis. A dose of 5 Gy can potentially produce mutant lines resistant to SRD in Phalaenopsis or other plants, too.

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Identification and Characterization of Soft Rot Bacterial Pathogens on Phalaenopsis Orchid in Bali

Cited by 2 publications

References 8 publications

Potensi dan Keragaman Bakteri Endofit sebagai Agens Pemacu Pertumbuhan dan Biokontrol Anggrek

Potensi dan Keragaman Bakteri Endofit sebagai Agens Pemacu Pertumbuhan dan Biokontrol Anggrek

Inoculation Methods to Determine Resistance of Phalaenopsis amabilis (L.) Regeneratded from Irradiated Protocorms to Dickeya dadantii

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