Identification and characterization of six peroxiredoxin transcripts from mud crab Scylla paramamosain : The first evidence of peroxiredoxin gene family in crustacean and their expression profiles under biotic and abiotic stresses

Tu, Dan Dan; Zhou, Yi; Gu, Wen Bin; Zhu, Qi; Xu, Bin; Zhou, Zhong Kai; Liu, Ze Peng; Wang, Cong; Chen, Yu Yin; Miao, Shuying

doi:10.1016/j.molimm.2017.11.029

Cited by 18 publications

(8 citation statements)

References 88 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The identified NdPrx3 cDNA sequence contained 684 bp ORF which encoded 24.7 kDa protein within the average size (20–30 kDa) of Prxs [42] . Prediction and comparison results of NdPrx3 conserve domain showed it is most common with some reported Prx3 in crustaceans, including H. americanus [43] , Scylla paramamosain [44] , Trinorchestia longiramus [45] , and Armadillidium nasatum [46] . For its existence in mitochondria, the homology of Prx3 was high in different species.…”

Section: Discussionmentioning

confidence: 83%

Molecular characterization and functional analysis of peroxiredoxin 3 (NdPrx3) from Neocaridina denticulata sinensis

Wang

Zhang

et al. 2023

Fish and Shellfish Immunology Reports

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 83%

Molecular characterization and functional analysis of peroxiredoxin 3 (NdPrx3) from Neocaridina denticulata sinensis

Wang

Zhang

et al. 2023

Fish and Shellfish Immunology Reports

View full text Add to dashboard Cite

“…These motifs are required for the enzyme's function, acting as the catalytic site and a resolving residue, respectively (Duan et al, 2013;Wan et al, 2014). CsPrx6 only has one motif (PVCTTE) in the N-terminus, which is similar to the Prx6 from certain insect species, clawed frog, and human (Duan et al, 2013;Fisher, 2011;Manevich and Fisher, 2005;Tu et al, 2018). Correspondingly, the motif conservation analysis and multiple alignments indicated that CsPrx5 is a member of the atypical 2-Cys subgroup and CsPrx6 is a member of the 1-Cys subgroup of Prxs.…”

Section: Expression Of Csprx5 and Csprx6 Under The Stress Of The Trap...mentioning

confidence: 99%

“…BInsects encounter a great variety of potentially damaging environmental factors, which inevitably lead to oxidative stress. Aerobic organisms generate reactive oxygen species (ROS) via their endogenous metabolism; however, excess ROS accumulation can damage biological macromolecules, disturb cellular processes, and prolong oxidative stress, thus leading to disease, aging, and cell death (Liu et al, 2023; Pulido and Parrish, 2003; Tu et al, 2018; Xian et al, 2014). In organisms, ROS levels usually increase markedly in response to environmental stress‐induced disruption of homeostasis and cellular function, as the products of organismal physiological metabolism (Inupakutika et al, 2016; Sanchez‐Hernandez et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Prxs are ubiquitous proteins that play a significant role to reduce peroxides, serving as the site of oxidation via an active site cysteine (Cys) residue (Karplus and Hall, 2007; Rhee, 2016; Sharapov et al, 2014). Humans, mice, nematodes, crustaceans, and fishes all contain Prxs (Chen et al, 2020; Helm et al, 2018; Ma et al, 2016; Tu et al, 2018). Prxs are classified by their structure, subcellular location, and the mode of action.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identification and characterization of Prx5 and Prx6 in Chilo suppressalis in response to environmental stress

et al. 2023

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

The antioxidant proteins, peroxiredoxins (Prxs), function to protect insects from reactive oxygen species‐induced toxicity. In this study, two Prx genes, CsPrx5, and CsPrx6, were cloned and characterized from the paddy field pest, Chilo suppressalis, containing open reading frames of 570 and 672 bp encoding 189 and 223 amino acid polypeptides, respectively. Then, we investigated the influence of various stresses on their expression levels using quantitative real‐time PCR (qRT‐PCR). The results showed expression of CsPrx5 and CsPrx6 in all developmental stages, with eggs having the highest level. CsPrx5 and CsPrx6 showed higher expression in the epidermis and fat body, and CsPrx6 also showed higher expression in midgut, fat body, and epidermis. Increasing concentrations of insecticides (chlorantraniliprole and spinetoram) and hydrogen peroxide (H2O2) increased the expression levels of CsPrx5 and CsPrx6. In addition, the expression levels of CsPrx5 and CsPrx6 were almost markedly upregulated in larvae under temperature stress or fed by vetiver. Thus, CsPrx5 and CsPrx6 upregulation might increase the C. suppressalis defense response by reducing the impact of environmental stress, providing a better understanding of the relationship between environmental stresses and insect defense systems.

show abstract

“…Presently, PRDX1-PRDX6 have been found to contain a 1-Cys PRDX group and 2-Cys PRDX group. PRDX1-5 belongs to the 2-Cys group, and PRDX6 belongs to the 1-Cys PRDX group 18,19. Besides helping to protect cells against oxidative stress (OS), PRDX6 uniquely possesses calcium independent phospholipase A2 (PLA2) activity and glutathione peroxidase activity that can help to prevent oxidative stress 20.…”

Section: Introductionmentioning

confidence: 99%

<p>Differential Expression And Effects Of Peroxiredoxin-6 On Drug Resistance And Cancer Stem Cell-Like Properties In Non-Small Cell Lung Cancer</p>

Xu¹,

Su²,

Gao³

et al. 2019

OTT

View full text Add to dashboard Cite

ObjectiveCancer stem-like cells (CSC) are thought to be involved in the cisplatin resistance of tumors. This study was designed to investigate the effect of PRDX6 on CSCs present in cisplatin-resistant non-small cell lung cancer (NSCLC) tumors.Materials and methodsCD133+/ABCG2+ H1299 CSCs and A549 CSCs were isolated. The IC50 values for cisplatin in treatment of CSCs were detected using the CCK8 assay. Then the isolated cells were identified using CD133. Wnt/β-catenin expression was evaluated by Western blot assays. Specimens of tumor and adjacent para-carcinoma tissue were collected from 30 NSCLC patients and examined by immunohistochemistry (IHC), qRT-PCR, and Western blotting to determine and compare their levels of PRDX6 and CD133 expression. Finally, siRNA-mediated silencing of PRDX6 was employed with both types of CSCs to determine the impact of PRDX6 on CD133 enrichment by flow cytometry, cell viability, and sphere formation ability.ResultsHigh levels of PRDX6 and CD133 expression were detected in samples of tumor tissue from NSCLC patients, and expression of PRDX6 and CD13 presented a positive relationship. Increasing levels of cisplatin resistance and upregulated levels of PRDX6, ABCG2, Wnt, and β-catenin expression were detected in CD133+/ABCG2+ H1299 and A549 CSCs. Transfection with siRNA targeting PRDX6 changed these cellular characteristics by decreasing the levels of PRDX6, ABCG2, Wnt, and β-catenin expression. We further demonstrated that exogenous silencing of PRDX6 effectively inhibited the sphere formation ability of CSCs and re-sensitized them to cisplatin.ConclusionOur results strongly suggest that PRDX6 promotes cisplatin resistance in human lung cancer cells by promoting the stem-like properties of cancer cells. Our findings also suggest PRDX6 as a target for treating cisplatin resistant NSCLC.

show abstract

Identification and characterization of six peroxiredoxin transcripts from mud crab Scylla paramamosain : The first evidence of peroxiredoxin gene family in crustacean and their expression profiles under biotic and abiotic stresses

Cited by 18 publications

References 88 publications

Molecular characterization and functional analysis of peroxiredoxin 3 (NdPrx3) from Neocaridina denticulata sinensis

Molecular characterization and functional analysis of peroxiredoxin 3 (NdPrx3) from Neocaridina denticulata sinensis

Identification and characterization of Prx5 and Prx6 in Chilo suppressalis in response to environmental stress

<p>Differential Expression And Effects Of Peroxiredoxin-6 On Drug Resistance And Cancer Stem Cell-Like Properties In Non-Small Cell Lung Cancer</p>

Contact Info

Product

Resources

About