Identification and Characterization of Sex-Biased MicroRNAs in Bactrocera dorsalis (Hendel)

Peng, Wei; Tariq, Kaleem; Xie, Junfei; Zhang, Hongyu

doi:10.1371/journal.pone.0159591

Cited by 21 publications

(19 citation statements)

References 61 publications

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“…melanogaster , where expression was preferentially associated with the reproductive functions [ 78 ]. More recent work that compared miRNA expression between mature male and female reproductive organs in Bactrocera dorsalis demonstrated that sex-biased miRNAs are likely involved in sexual differentiation [ 79 ]. Furthermore, the expression of miRNAs varied across developmental stages of Xenopus laevis [ 80 ] and across different human tissues [ 81 ].…”

Section: Discussionmentioning

confidence: 99%

Differentially expressed microRNAs associated with changes of transcript levels in detoxification pathways and DDT-resistance in the Drosophila melanogaster strain 91-R

Seong¹,

Coates²,

Kim³

et al. 2018

PLoS ONE

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Dichloro-diphenyl-trichloroethane (DDT) resistance among arthropod species is a model for understanding the molecular adaptations in response to insecticide exposures. Previous studies reported that DDT resistance may involve one or multiple detoxification genes, such as cytochrome P450 monooxygenases (P450s), glutathione S-transferases (GSTs), esterases, and ATP binding cassette (ABC) transporters, or changes in the voltage-sensitive sodium channel. However, the possible involvement of microRNAs (miRNAs) in the post-transcriptional regulation of genes associated with DDT resistance in the Drosophila melanogaster strain 91-R remains poorly understood. In this study, the majority of the resulting miRNAs discovered in small RNA libraries from 91-R and the susceptible control strain, 91-C, ranged from 16–25 nt, and contained 163 precursors and 256 mature forms of previously-known miRNAs along with 17 putative novel miRNAs. Quantitative analyses predicted the differential expression of ten miRNAs between 91-R and 91-C, and, based on Gene Ontology and pathway analysis, these ten miRNAs putatively target transcripts encoding proteins involved in detoxification mechanisms. RT-qPCR validated an inverse correlation between levels of differentially-expressed miRNAs and their putatively targeted transcripts, which implies a role of these miRNAs in the differential regulation of detoxification pathways in 91-R compared to 91-C. This study provides evidence associating the differential expression of miRNAs in response to multigenerational DDT selection in Drosophila melanogaster and provides important clues for understanding the possible roles of miRNAs in mediating insecticide resistance traits.

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Section: Discussionmentioning

confidence: 99%

Differentially expressed microRNAs associated with changes of transcript levels in detoxification pathways and DDT-resistance in the Drosophila melanogaster strain 91-R

Seong¹,

Coates²,

Kim³

et al. 2018

PLoS ONE

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show abstract

“…As a dipteran insect, B. dorsalis is a typical holometabolous insect that undergoes larval–pupal metamorphosis. Additionally, several studies have shown that miRNAs are involved in B. dorsalis development and reproduction (Huang et al, 2014; Peng et al, 2016, 2017). Considering the diverse roles of miRNAs, some miRNAs in the regulation of gene expression during metamorphosis probably exist in B. dorsalis .…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Analysis of MicroRNAs in Relation to Pupariation in Oriental Fruit Fly

et al. 2019

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Insect metamorphosis is a complex process involving drastic morphological and physiological changes. microRNAs (miRNAs) are a class of endogenous small non-coding RNAs that play key roles in regulating various biological processes, including metamorphosis, by post-transcriptional repression of mRNAs. The oriental fruit fly, Bactrocera dorsalis , is one of the most destructive insect pests in many Asian countries and the Pacific Islands. The regulatory role of miRNAs in B. dorsalis metamorphosis is unclear. To better understand the molecular regulatory mechanisms of miRNAs in pupariation, Illumina sequencing of the wandering stage (WS), the late WS and the white puparium stage of B. dorsalis were performed. Two hundred forty-nine miRNAs, including 184 known miRNAs and 65 novel miRNAs, were obtained. Among these miRNAs, 19 miRNAs were differentially expressed in pupariation, and eight miRNAs showed relative high expression levels (>50 TPM), of which five differentially expressed miRNAs (DEMs) had target differentially expressed genes (DEGs) predicted by the expected miRNA–mRNA negative regulation pattern using the Illumina HiSeq data. Four sets of DEMs and their predicted target DEGs were confirmed by qPCR. Of the four miRNAs, two miRNAs were down-regulated: miR-981, which may target pdpc , and Bdo-novel-mir-55, which potentially regulates spsX1, psB/C , and chit3 . The other two miRNAs were up-regulated: let-7a-3p, which possibly controls lap , and Bdo-novel-mir-24, which may regulate ipc and sp1/2 . This study provides a useful resource to elucidate the regulatory role of miRNAs and understand the molecular mechanisms of metamorphosis.

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“…Various insects such as Drosophila melanogaster [ 7 ], Bombyx mori [ 8 ], Blattella germanica [ 9 ], Apis mellifera [ 10 ] and Aedes aegypti [ 11 ] have also been reported to encode miRNAs that play important roles in development, adaptation and host-virus interactions. The analysis of small RNA libraries generated from various developmental stages in different species enables an in-depth study of miRNA in physiological processes [ 12 ]. In insects, including D .…”

Section: Introductionmentioning

confidence: 99%

Characterization and comparative analysis of microRNAs in the rice pest Sogatella furcifera

et al. 2018

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MicroRNAs (miRNAs) are a class of endogenous regulatory RNA molecules 21-24 nucleotides in length that act as functional regulators of post-transcriptional repression of messenger RNA. We report the identification and characterization of a conserved miRNA and 171 novel miRNAs in the migratory rice pest Sogatella furcifera by deep sequencing, which were observed to be biased towards female adults of the insect, modulating the functionality and targets of the miRNAs in sex differentiation. A switch in arm usage was also observed in 9 miRNA when compared to the insect ancestor during insect evolution. The miRNA loci showed high 5’ fidelity in both miRNA and star species and about 93.4% of WBPH miRNAs conserved within non-planthopper species were homologous with planthopper species. The novel miRNAs identified in this study provide a better understanding of the sRNA and the regulatory role of miRNA in sexual dimorphism and alteration in the expression or function of miRNAs in the rice pest.

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Identification and Characterization of Sex-Biased MicroRNAs in Bactrocera dorsalis (Hendel)

Cited by 21 publications

References 61 publications

Differentially expressed microRNAs associated with changes of transcript levels in detoxification pathways and DDT-resistance in the Drosophila melanogaster strain 91-R

Differentially expressed microRNAs associated with changes of transcript levels in detoxification pathways and DDT-resistance in the Drosophila melanogaster strain 91-R

Genome-Wide Analysis of MicroRNAs in Relation to Pupariation in Oriental Fruit Fly

Characterization and comparative analysis of microRNAs in the rice pest Sogatella furcifera

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