Identification and characterization of plant-specific NAC gene family in canola (Brassica napus L.) reveal novel members involved in cell death

Abstract

NAC transcription factors are plant-specific and play important roles in plant development processes, response to biotic and abiotic cues and hormone signaling. However, to date, little is known about the NAC genes in canola (or oilseed rape, Brassica napus L.). In this study, a total of 60 NAC genes were identified from canola through a systematical analysis and mining of expressed sequence tags. Among these, the cDNA sequences of 41 NAC genes were successfully cloned. The translated protein sequences of canola NAC genes with the NAC genes from representative species were phylogenetically clustered into three major groups and multiple subgroups. The transcriptional activities of these BnaNAC proteins were assayed in yeast. In addition, by quantitative real-time RT-PCR, we further observed that some of these BnaNACs were regulated by different hormone stimuli or abiotic stresses. Interestingly, we successfully identified two novel BnaNACs, BnaNAC19 and BnaNAC82, which could elicit hypersensitive response-like cell death when expressed in Nicotiana benthamiana leaves, which was mediated by accumulation of reactive oxygen species. Overall, our work has laid a solid foundation for further characterization of this important NAC gene family in canola.

Application of protoplast technology to CRISPR/Cas9 mutagenesis: from single‐cell mutation detection to mutant plant regeneration

“…() described a rapeseed protoplast isolation protocol, and plantlets could be regenerated from these protoplasts. A rapeseed protoplast transient transfection system has also been established (Wang et al ., ). Our results indicate that the rapeseed protoplast genome can be mutated by CRISPR/Cas9.…”

Genome-wide survey of switchgrass NACs family provides new insights into motif and structure arrangements and reveals stress-related and tissue-specific NACs

“…All NAC proteins have relatively conserved NAC domains in the N-terminus with five (A-E) subdomains among which DBDs were contained in subdomains D & E, while subdomains B, C, and E corresponded to the proteins’ diverse functional roles for cooperated DNA-binding specificity of NACs 6, 60, 70 . A previous study showed that subdomains A & E were necessary for stable NAC homo- or hetero-dimer formation, while subdomains D & E indispensable for DNA-binding 71 .…”

Antibiotic resistance genes in different animal manures and their derived organic fertilizer

“…and Zhang et al, study described that cattle manure application increased the abundance of ARGs in plant root, while poultry manure application increased ARGs in rhizosphere, root endophyte and phyllosphere [17]. Thus, the continuous increase or high persistence of ARGs in livestock environments may pose potential threats to human health and the ecological environment [18,19].…”