Identification and characterization of phospholipases A2 from the skin secretion of Pithecopus azureus anuran

Souza, Beatriz Blenda Pinheiro de; Fh, José L. Cardozo; Murad, André M.; Prates, Maura V.; Coura, Marcelo M.A.; Brand, Guilherme D.; Barbosa, Éder Alves; Bloch, Carlos

doi:10.1016/j.toxicon.2019.06.002

Cited by 4 publications

(2 citation statements)

References 57 publications

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“…Moreover, relevant enzymatic activities were detected in the skin secretion, such as fibrinogenolytic, hyaluronidasic and metallopeptidasic. Enzymes presenting such activities have already been described as important toxins for snake venoms [81,82] and were also described in some amphibians from different genus, for example phospholipase in Pithecopus azureus [83] and serine peptidases in Duttaphrynus melanostictus [84]. Furthermore, Fusco et al 2020 [85] studied the epidermal secretion of Argenteohyla siemersi and described both phospholipasic and hemolytic activities.…”

Section: Amphibianmentioning

confidence: 97%

Neglected Venomous Animals and Toxins: Underrated Biotechnological Tools in Drug Development

Coelho

Silva

Beraldo-Neto

et al. 2021

Toxins

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Among the vast repertoire of animal toxins and venoms selected by nature and evolution, mankind opted to devote its scientific attention—during the last century—to a restricted group of animals, leaving a myriad of toxic creatures aside. There are several underlying and justifiable reasons for this, which include dealing with the public health problems caused by envenoming by such animals. However, these studies became saturated and gave rise to a whole group of animals that become neglected regarding their venoms and secretions. This repertoire of unexplored toxins and venoms bears biotechnological potential, including the development of new technologies, therapeutic agents and diagnostic tools and must, therefore, be assessed. In this review, we will approach such topics through an interconnected historical and scientific perspective that will bring up the major discoveries and innovations in toxinology, achieved by researchers from the Butantan Institute and others, and describe some of the major research outcomes from the study of these neglected animals.

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Section: Amphibianmentioning

confidence: 97%

Neglected Venomous Animals and Toxins: Underrated Biotechnological Tools in Drug Development

Coelho

Silva

Beraldo-Neto

et al. 2021

Toxins

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show abstract

“…When peptides and bacterial membranes interact, the antimicrobial effect is often immediate, lysing the membrane through several membrane permeabilization mechanisms . There is a myriad of molecules with such an effect described in the literature, isolated from a wide variety of sources, including reptiles , and amphibians, , both prolific sources of these biomolecules. Cathelicidins have antibacterial activity against Klebsiella pneumoniae , Serratia marcescens , Enterobacter cloacae , E. coli , and P. aeruginosa , with minimum inhibitory concentrations (MIC) around 30 μg/mL .…”

mentioning

confidence: 99%

Mechanistic Insights into the Leishmanicidal and Bactericidal Activities of Batroxicidin, a Cathelicidin-Related Peptide from a South American Viper (Bothrops atrox)

et al. 2021

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Snake venoms are important sources of bioactive molecules, including those with antiparasitic activity. Cathelicidins form a class of such molecules, which are produced by a variety of organisms. Batroxicidin (BatxC) is a cathelicidin found in the venom of the common lancehead (Bothrops atrox). In the present work, BatxC and two synthetic analogues, BatxC(C-2.15Phe) and BatxC(C-2.14Phe)des-Phe1, were assessed for their microbicidal activity. All three peptides showed a broad-spectrum activity on Gram-positive and -negative bacteria, as well as promastigote and amastigote forms of Leishmania (Leishmania) amazonensis. Circular dichroism (CD) and nuclear magnetic resonance (NMR) data indicated that the three peptides changed their structure upon interaction with membranes. Biomimetic membrane model studies demonstrated that the peptides exert a permeabilization effect in prokaryotic membranes, leading to cell morphology distortion, which was confirmed by atomic force microscopy (AFM). The molecules considered in this work exhibited bactericidal and leishmanicidal activity at low concentrations, with the AFM data suggesting membrane pore formation as their mechanism of action. These peptides stand as valuable prototype drugs to be further investigated and eventually used to treat bacterial and protozoal infections.

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A first look at the N- and O-glycosylation landscape in anuran skin secretions

et al. 2022

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Identification and characterization of phospholipases A2 from the skin secretion of Pithecopus azureus anuran

Cited by 4 publications

References 57 publications

Neglected Venomous Animals and Toxins: Underrated Biotechnological Tools in Drug Development

Neglected Venomous Animals and Toxins: Underrated Biotechnological Tools in Drug Development

Mechanistic Insights into the Leishmanicidal and Bactericidal Activities of Batroxicidin, a Cathelicidin-Related Peptide from a South American Viper (Bothrops atrox)

A first look at the N- and O-glycosylation landscape in anuran skin secretions

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