Identification and Characterization of Phenylpropanoid Biosynthetic Genes and Their Accumulation in Bitter Melon (Momordica charantia)

Cường, Đỗ Mạnh; Kwon, Soon‐Jae; Jeon, Jin Pyeong; Park, Yun Ji; Park, Jong‐Seok; Park, Sang Un

doi:10.3390/molecules23020469

Cited by 19 publications

(16 citation statements)

References 31 publications

(38 reference statements)

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“…The reason is that high blue light in LED-mix greatly contributes to the increase of the secondary metabolites such as phenols and flavonoids as compared with other treatments [26, 82, 83]. More studies suggested that blue light should be recommended as a supplementary light source for accumulation of phenols and flavonoids [84, 85]. Moreover, the promotion of primary metabolism partly promoted contents of phenols and flavonoids in the LED-mix treatment (Table 3), since the products of primary metabolism are utilized to synthesize a wide range of secondary metabolites through the shikimate pathway, mevalonic acid pathway, and methylerythritol 4-phosphate (MEP) pathway in higher plants [86–89].…”

Section: Discussionmentioning

confidence: 99%

Supplementary Light Source Affects Growth, Metabolism, and Physiology ofAdenophora triphylla(Thunb.) A.DC. Seedlings

Liu

Ren

Jeong

2019

BioMed Research International

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Adenophora triphylla(Thunb.) A.DC., a well-known herbaceous medicinal species, has been reported to protect against human obesity, cancer, and inflammation. Supplementary lighting is a practical strategy to improve crop quality, especially at a propagation stage. However, there has been no study available on the optimal supplementary light source for the commercial production ofA. triphyllaseedlings. In this study, plug seedlings were cultivated in a greenhouse for four weeks under an average daily light intensity of 490μmol·m−2·s−1PPFD coming from the sun and a supplemental lighting (16 h per day) at 120μmol·m−2·s−1PPFD provided by high pressure sodium (HPS), metal halide (MH), far-red (FR) light, white LED (red: green: blue = 2:4:3, LED-w), or mixed (red: green: blue = 4:1:4) LED (LED-mix). The results showed that LED-mix, with a higher percentage of red and blue light, substantially promoted seedling growth compared to other treatments by increasing stem diameter, biomass, specific leaf weight, and root to shoot ratio. The LED-mix also promoted accumulation of soluble sugar, starch, and chlorophyll in the tissue and increased contents of total phenols and flavonoids. Moreover, stomata density and pore area per leaf area under the LED-mix were remarkably greater than those under other treatments. Furthermore, the Western blot analysis revealed that the expression of photosynthetic protein, D1, was notably enhanced by the LED-mix as compared with other light sources. In addition, the LED-mix alleviated the oxidative damage of seedlings by improving enzymatic and nonenzymatic antioxidant systems. Collectively, these results suggest that the LED-mix was the optimal supplementary light source for the production of highest qualityA. triphyllaseedlings.

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Section: Discussionmentioning

confidence: 99%

Supplementary Light Source Affects Growth, Metabolism, and Physiology ofAdenophora triphylla(Thunb.) A.DC. Seedlings

Liu

Ren

Jeong

2019

BioMed Research International

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“…In this study, a previously published compound extraction method was used [26] with some modifications. Phenylpropanoids were extracted by adding 2 mL MeOH (80%) to each sample (100 mg), vortexing and incubating for 1 h in a sonicator (Branson Ultrasonic Co., Danbury, CT, USA), then centrifuging (12,000 rpm at 4 • C) for 15 min.…”

Section: Analysis Of Phenylpropanoid Content By Hplcmentioning

confidence: 99%

Effect of Salinity Stress on Phenylpropanoid Genes Expression and Related Gene Expression in Wheat Sprout

et al. 2020

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The effect of salinity (NaCl treatment) on the nutritive value of wheat sprouts was investigated by analyzing the expression of phenylpropanoid biosynthetic pathway genes and the levels of phenylpropanoid compounds. Treatment with various concentrations of NaCl (50, 100, and 200 mM) resulted in increased epicatechin levels but decreased accumulation of catechin hydrate, benzoic acid, and quercetin compounds in the sprouts compared with the control (0 mM). The trans-cinnamic acid, 4-hydroxybenzoic acid, ferulic acid, epicatechin, and total phenylpropanoid level in wheat sprout was the highest at 50 mM of NaCl treatment. Six-day-old wheat plantlets exposed to 50 mM NaCl for 6, 12, 24, 48, and 72 h, showed that the total phenylpropanoids accumulation was the highest at 48 h after the treatment and most of the treatments showed higher phenylpropanoid content than the control at the same time points. Although the shoot and root length and the fresh weight of wheat sprouts decreased with NaCl treatment, these results suggest that treatment of 50 mM NaCl improves the nutritional quality of wheat sprouts, due to increased phenylpropanoid concentrations.

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“…Samples of wheat sprouts were extracted and subjected to HPLC analysis of soluble phenolic compounds using the method by Cuong et al, (2018) with slight modifications [27]. Prior to HPLC analysis, samples of wheat sprouting were freeze-dried at −80 • C for at least 48 h, and then ground into a fine powder using mortar and pestle.…”

Section: Analysis Of Phenylpropanoid Content By Hplcmentioning

confidence: 99%

“…After centrifugation at 10,000 g for 15 min, the supernatants were filtered through 0.45-µm filters for HPLC analysis. The conditions and gradient program for HPLC, used in this study, have previously been described by Cuong et al, (2018) [27]. HPLC analysis was performed with a C18 column (250 mm × 4.6 mm, 5 µm; RStech; Daejeon, Korea).…”

Section: Analysis Of Phenylpropanoid Content By Hplcmentioning

confidence: 99%

Effects of LED lights on Expression of Genes Involved in Phenylpropanoid Biosynthesis and Accumulation of Phenylpropanoids in Wheat Sprout

Cường

Park

et al. 2019

Agronomy

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Phenylpropanoids are naturally occurring compounds that exert beneficial pharmacological effects on human health. Phenylpropanoids can act as antioxidants and are involved in resistance to ultraviolet light and cancer; these compounds possess anti-inflammatory, antiviral, and antibacterial activity, and aid in wound healing. The expression of genes involved in phenylpropanoid biosynthesis, and consequent accumulation of phenylpropanoids in wheat sprout under conditions of stress, have not been extensively studied. This is the first study to examine the effects of light-emitting diodes (LED) on the expression of genes involved in phenylpropanoid biosynthesis and accumulation of phenylpropanoids in wheat sprouts. Our results, obtained using qRT-PCR and HPLC analyses, indicate that white light (380 nm) was the optimal wavelength for epicatechin biosynthesis in wheat sprouts. Compared with the effects of white light, blue light (470 nm) enhanced the accumulation of gallic acid and quercetin, but decreased the levels of p-coumaric acid and epicatechin; red light (660 nm) increased the accumulation of ferulic acid at 8 day and p-coumaric acid at 12 day. Compared gene expression with phenylpropanoid content showed that TaPAL3, TaPAL4, and TaDFR maybe important genes in phenylpropanoid biosynthesis in wheat sprout. This study provides insights into the effects of led lights on phenylpropanoid production in wheat sprouts. This knowledge will help improve secondary metabolite production in wheat sprouts.

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Identification and Characterization of Phenylpropanoid Biosynthetic Genes and Their Accumulation in Bitter Melon (Momordica charantia)

Cited by 19 publications

References 31 publications

Supplementary Light Source Affects Growth, Metabolism, and Physiology ofAdenophora triphylla(Thunb.) A.DC. Seedlings

Supplementary Light Source Affects Growth, Metabolism, and Physiology ofAdenophora triphylla(Thunb.) A.DC. Seedlings

Effect of Salinity Stress on Phenylpropanoid Genes Expression and Related Gene Expression in Wheat Sprout

Effects of LED lights on Expression of Genes Involved in Phenylpropanoid Biosynthesis and Accumulation of Phenylpropanoids in Wheat Sprout

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