Identification and characterization of P15RS, a novel P15INK4b related gene on G1/S progression

Li, Jun; Huitu, Liu; Zhang, Xue; Gao, Ping; Wang, Juan; Hu, Zhijun

doi:10.1016/s0006-291x(02)02684-0

Cited by 17 publications

(27 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S3). This result is in consistent with the notion that p15RS functions as a negative regulator in the G 1 phase in cell cycle control (19). We speculate that p15RS may play a role in tumorigenesis.…”

Section: Discussionsupporting

confidence: 92%

“…A previous report showed that the knockdown of p15RS in MLIK6 cells up-regulates the expression of CYCLIN D1 (19), indicating that p15RS inhibits CYCLIN D1 expression. Because CYCLIN D1 is a direct target gene transactivated by the ␤-catenin⅐LEF-1 complex through a LEF-1 binding site in its promoter (20), we speculated that p15RS may negatively regulate CYCLIN D1 expression via the ␤-catenin⅐LEF-1 complex.…”

Section: Discussionmentioning

confidence: 93%

“…P15RS, a novel p15INK4b-related gene involved in G 1 /S progression, was identified by a differential display experiment from cells overexpressing p15INK4b (19). Antisense p15RS was shown to up-regulate the expression of CYCLIN D1 in MLIK6 cells.…”

mentioning

confidence: 99%

“…Antisense p15RS was shown to up-regulate the expression of CYCLIN D1 in MLIK6 cells. Therefore, p15RS was regarded as a negative regulator in cell cycle control in G 1 phase by down-regulating CYCLIN D1 expression (19). Because CYCLIN D1 is a direct target gene transactivated by the ␤-catenin⅐TCF4/LEF-1 complex through a LEF-1 binding site in its promoter (20), we speculated that p15RS might be involved in regulating Wnt/␤-catenin signaling.…”

mentioning

confidence: 99%

See 3 more Smart Citations

p15RS Attenuates Wnt/β-Catenin Signaling by Disrupting β-Catenin·TCF4 Interaction

Zhang

et al. 2010

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

The formation of a ␤-catenin⅐TCF4 complex in the nucleus of cells is well known as a prerequisite for the transcription of Wnt target genes. Although many co-factors have been identified to regulate the activity of the ␤-catenin⅐TCF4 complex, it remains unclear how the complex association is negatively regulated. In this study, we report that p15RS, a negative regulator of the cell cycle, blocks ␤-catenin⅐TCF4 complex formation and inhibits Wnt signaling. We observed that p15RS interacts with ␤-catenin and TCF4. Interestingly, whereas the interaction of p15RS with ␤-catenin is increased, its interaction with TCF4 is decreased upon Wnt1 stimulation. Moreover, overexpression of p15RS reduces the interaction of ␤-catenin with TCF4, whereas the depletion of p15RS enhances their interaction. We further demonstrate that overexpression of p15RS suppresses canonical Wnt signaling and results in retarded cell growth, whereas depletion of p15RS shows an enhanced effect on Wnt signaling. We analyzed that inhibition of Wnt signaling by p15RS leads to decreased expression of CYCLIN D1 and c-MYC, two Wnt targeted genes critical for cell growth. Our data suggest that p15RS inhibits Wnt signaling by interrupting ␤-catenin⅐TCF4 complex formation and that Wnt signaling initiates downstream gene expression by removing p15RS from promoters.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 93%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

p15RS Attenuates Wnt/β-Catenin Signaling by Disrupting β-Catenin·TCF4 Interaction

Zhang

et al. 2010

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…33 Previous studies showed that RPRD1A, also called P15RS, regulates cell cycle genes and attenuates WNT signaling, although the mechanism was unclear. 34,35 The interaction of RPRD1A with RNAP II has not been reported previously, and the RPRD1B and RPRD2 proteins are functionally uncharacterized.…”

mentioning

confidence: 96%

Control of the RNA polymerase II phosphorylation state in promoter regions by CTD interaction domain-containing proteins RPRD1A and RPRD1B

Ni¹,

Olsen²,

Guo³

et al. 2011

Transcription

View full text Add to dashboard Cite

CREPT and p15RS regulate cell proliferation and cycling in chicken DF‐1 cells through the Wnt/β‐catenin pathway

Jin

Chen

Zuo

et al. 2017

J of Cellular Biochemistry

View full text Add to dashboard Cite

The CREPT (cell cycle-related and expression elevated protein in tumor, also known as RPRD1B) and p15RS (p15 -related sequence, also known as RPRD1A) have been shown to regulate cell proliferation and alter the cell cycle through Wnt/β-catenin pathway downstream genes in human. Although several studies have revealed the mechanism by which CREPT and p15RS regulate cell proliferation in human and mammals, it is still unclear how these genes function in poultry. In order to determine the function of CREPT and p15RS in chicken, we examined the expression of CREPT and p15RS in a variety of chicken tissues and DF-1 cells. Then, we determined the effect of overexpression or depletion of CREPT or p15RS, by transiently transfecting chicken DF-1 cells with overexpression and short hairpin RNA (shRNA) vectors respectively, on the regulation of cell proliferation. The results showed that CREPT and p15RS had different expression patterns and opposite effects on the cell cycling and proliferation. Knockdown of p15RS expression or overexpression of CREPT facilitated cell proliferation by promoting the cell-cycle transition from G0/G1 to S-phase and G2/M, whereas knockdown of CREPT or overexpression of p15RS inhibited cell proliferation. Mechanistically, CREPT and p15RS control DF-1 cell proliferation by regulating the expression of Wnt/β-catenin pathway downstream regulatory genes, including β-catenin, TCF4, and Cyclin D1. In conclusion, CREPT and p15RS regulate cell proliferation and the cell-cycle transition in chicken DF-1 cells by regulating the transcription of Wnt/β-catenin pathway downstream regulatory genes.

show abstract

Identification and characterization of P15RS, a novel P15INK4b related gene on G1/S progression

Cited by 17 publications

References 18 publications

p15RS Attenuates Wnt/β-Catenin Signaling by Disrupting β-Catenin·TCF4 Interaction

p15RS Attenuates Wnt/β-Catenin Signaling by Disrupting β-Catenin·TCF4 Interaction

Control of the RNA polymerase II phosphorylation state in promoter regions by CTD interaction domain-containing proteins RPRD1A and RPRD1B

CREPT and p15RS regulate cell proliferation and cycling in chicken DF‐1 cells through the Wnt/β‐catenin pathway

Contact Info

Product

Resources

About