Identification and characterization of nuclear microsatellite loci in the aquatic moss <i>Platyhypnidium riparioides</i> (Brachytheciaceae)

Hutsemékers, Virginie; Risterucci, Ange-Marie; Ricca, Mariana; Boles, Sandra B.; Hardy, Olivier J.; Shaw, A. Jonathan; Vanderpoorten, Alain

doi:10.1111/j.1755-0998.2008.02193.x

Cited by 16 publications

(13 citation statements)

References 13 publications

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“…91 and 34 sympatric specimens of R. riparioides and G. torrenticola were collected at the type (and to date only known) locality of the latter in Tenerife (see online Appendix A). Each of the 1136 gametophytes collected was genotyped at eight microsatellite loci using primers R3, R9, R11, R13 and R17 following the protocols described in Hutsemekers et al (2008). Another three primer pairs was designed from the library of SSR-enriched loci described in Hutsemekers et al (2008).…”

Section: Taxon Sampling Morphological Analyses and Molecular Protocolsmentioning

confidence: 99%

“…Each of the 1136 gametophytes collected was genotyped at eight microsatellite loci using primers R3, R9, R11, R13 and R17 following the protocols described in Hutsemekers et al (2008). Another three primer pairs was designed from the library of SSR-enriched loci described in Hutsemekers et al (2008). These forward and reverse primer pairs are: R21: F: CCCAAATGCAATCCATGA; R: GACGAAGCCGAAACTCGT; R24: F: TCCTCTTGGTTTGAAAAGG; R: GCAGGTGAAATCGAAAGA; R26: F: CGCACTACCGATCTATGC; R: TTTTGCAGTTTCCTCACC.…”

Section: Taxon Sampling Morphological Analyses and Molecular Protocolsmentioning

confidence: 99%

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Morphology informed by phylogeny reveals unexpected patterns of species differentiation in the aquatic moss Rhynchostegium riparioides s.l.

Hutsemékers

Vieira

Ros

et al. 2012

Molecular Phylogenetics and Evolution

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a b s t r a c tBryophyte floras typically exhibit extremely low levels of endemism. The interpretation, that this might reflect taxonomic shortcomings, is tested here for the Macaronesian flora, using the moss species complex of Rhynchostegium riparioides as a model. The deep polyphyly of R. riparioides across its distribution range reveals active differentiation that better corresponds to geographic than morphological differences. Morphometric analyses are, in fact, blurred by a size gradient that accounts for 80% of the variation observed among gametophytic traits. The lack of endemic diversification observed in R. riparioides in Macaronesia weakens the idea that the low rates of endemism observed in the Macaronesian bryophyte flora might solely be explained by taxonomic shortcomings. To the reverse, the striking polyphyly of North American and European lineages of R. riparioides suggests that the similarity between the floras of these continents has been over-emphasized. Discriminant analyses point to the existence of morphological discontinuities among the lineages resolved by the molecular phylogeny. The global rate of error associated to species identification based on morphology (0.23) indicates, however, that intergradation of shape and size characters among species in the group challenges their identification.

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Section: Taxon Sampling Morphological Analyses and Molecular Protocolsmentioning

confidence: 99%

Section: Taxon Sampling Morphological Analyses and Molecular Protocolsmentioning

confidence: 99%

Morphology informed by phylogeny reveals unexpected patterns of species differentiation in the aquatic moss Rhynchostegium riparioides s.l.

Hutsemékers

Vieira

Ros

et al. 2012

Molecular Phylogenetics and Evolution

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“…Investigations on spatial genetic structure rely on the use of highly variable genetic markers, such as microsatellite or AFLP markers (e.g., Snäll et al 2004a). Although taxon-specific microsatellites are presently widely available for many kinds of organisms, there have been, so far, only few reports of the successful development of species-specific microsatellite markers for bryophytes (e.g., Van Der Velde et al 2000;Leonardia et al 2006;Provan and Wilson 2007;Stackelberg et al 2006;Hutsemekers et al 2008b;Korpelainen et al 2008a, b;Szövenyi et al 2008), among which markers developed for liverworts are almost nonexistent (but see, Korpelainen et al 2008a). Therefore, to improve knowledge of population genetic processes in liverworts, especially regarding spatial genetic structuring, we examined fine-scale spatial genetic structure in the small (\3 cm long) liverwort, Barbilophozia attenuata (Mart.)…”

Section: Introductionmentioning

confidence: 99%

Fine-scale spatial genetic structure of a liverwort (Barbilophozia attenuata) within a network of ant trails

et al. 2010

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Fine-scale spatial genetic structure (SGS) of the liverwort, Barbilophozia attenuata, occupying an area characterized by a network of ant trails, was investigated using microsatellite markers. This is the first study investigating SGS in a liverwort. Significant genetic differentiation was detected among colonies along and outside ant trails, and the SGS pattern varied depending on the spatial scale. At short distances, kinship coefficients were significantly positive up to about eight meters, after which they approached zero and turned negative, while at distances greater than 25 m the values were about zero. Thus, nearby individuals are more closely related than expected, at mid-distances less related, and at great distances genotypes show a random distribution. We suggest that the reproductive mode strongly affects SGS in B. attenuata. Asexual propagation by relatively large gemmae allows more effective establishment than sexual reproduction by small-sized spores, and causes an aggregation of similar genotypes, although the inbreeding effect cannot be ruled out. In environments with small-scale disturbances, e.g., ant trails, gemmae are favoured over spores at establishment. Also, the diaspore bank of the forest floor can be activated by disturbances, which may affect SGS. At mid-distances, the isolation by distance effect, presumably related to comparatively ineffective gemma dispersal, is visible, while at greater distances, the role of spores as effective means of dispersal is evident. The Sp statistic values, which quantify the strength of SGS, indicate that outsider colonies possess less SGS than do plant colonies along ant trails, which relates to the more frequent spore production of outsider colonies. Moreover, dispersal from fallen logs or stumps may be more effective than dispersal from ground-level colonies along ant trails.

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“…Each gametophyte was genotyped at 10 microsatellite loci using primers R3, R9, R11, R13, R14, and R17 (36) and four new primer pairs designed from the library of SSR-enriched loci described in ref. 36: R21: F, CCCAAATGCAATCCATGA and R, GACGAAGCCGAAACTCGT; R24: F, TCCT-CTTGGTTTGAAAAGG and R, GCAGGTGAAATCGAAAGA; R26: F, CGCACTA-CCGATCTATGC and R, TTTTGCAGTTTCCTCACC; and R35: F, ACTCTCGTCGT-TCATTTT and R, AATCAATCGTGAACTTTGCT.…”

Section: Methodsmentioning

confidence: 99%

“…DNA extraction and genotyping followed the protocols described in ref. 36. Each gametophyte was genotyped at 10 microsatellite loci using primers R3, R9, R11, R13, R14, and R17 (36) and four new primer pairs designed from the library of SSR-enriched loci described in ref.…”

Section: Methodsmentioning

confidence: 99%

Oceanic islands are not sinks of biodiversity in spore-producing plants

Hutsemékers

Szövényi

Shaw

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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Islands have traditionally been considered as migratory and evolutionary dead ends for two main reasons: island colonizers are typically assumed to lose their dispersal power, and continental back colonization has been regarded as unlikely because of niche preemption. The hypothesis that islands might actually represent dynamic refugia and migratory stepping stones for species that are effective dispersers, and in particular, for spore-producing plants, is formally tested here, using the archipelagos of the Azores, Canary Islands, and Madeira, as a model. Population genetic analyses based on nuclear microsatellite variation indicate that dispersal ability of the moss Platyhypnidium riparioides does not decrease in the island setting. The analyses further show that, unlike island populations, mainland (southwestern Europe and North Africa) populations underwent a severe bottleneck during the last glacial maximum (LGM). Our results thus refute the traditional view of islands as the end of the colonization road and point to a different perception of North Atlantic archipelagos as major sources of biodiversity for the postglacial recolonization of Europe by spore-producing plants.biogeography | speciation | genetic diversity | island syndromes | isolation-with-migration I sland biogeography has historically been and remains a research area of prime importance for the advance of biology (1). It was in an island setting that Darwin (2) and Wallace (3) presented their theories of evolution by natural selection. It was also in this context that McArthur and Wilson (4) described the "equilibrium model," according to which biodiversity patterns can be explained by isolation from source populations (immigration) and area size (extinction). Oceanic islands hence appear as natural laboratories of evolutionary and biogeographic processes and Wallace (5) suggested that understanding evolutionary processes within an island context is a key to understanding the same mechanisms in the more complex continental settings.Theoretical work on population structure has used island models as paradigms for understanding how interactions between gene flow, effective population size, and genetic drift influence patterns of genetic variation. Although surprisingly few studies have explicitly tested models of population structure and diversity in island and mainland plant populations (6), theory predicts that island populations should be less variable and more genetically differentiated relative to source populations (7). Differentiation of island populations is further enhanced by one of the most striking syndromes displayed by island taxa, that is, their typical loss of dispersal power as a result of either absence of predation and/or competition in the island setting or counter selection against high dispersal ability associated with the risk of massive individual loss to surrounding sea water (8). This process can be fairly rapid. In the annual daisy Lactuca muralis, for example, a substantial and significant loss of dispersal power owing...

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Identification and characterization of nuclear microsatellite loci in the aquatic moss Platyhypnidium riparioides (Brachytheciaceae)

Cited by 16 publications

References 13 publications

Morphology informed by phylogeny reveals unexpected patterns of species differentiation in the aquatic moss Rhynchostegium riparioides s.l.

Morphology informed by phylogeny reveals unexpected patterns of species differentiation in the aquatic moss Rhynchostegium riparioides s.l.

Fine-scale spatial genetic structure of a liverwort (Barbilophozia attenuata) within a network of ant trails

Oceanic islands are not sinks of biodiversity in spore-producing plants

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