Identification and characterization of new <i>Muscodor</i> endophytes from gramineous plants in Xishuangbanna, China

Mao, Lijuan; Chen, Jiajie; Xia, Chenyang; Feng, Xiaoxiao; Kong, Dedong; Qi, Zhenyu; Liu, Feng; Dian, Chen; Lin, Fu‐Cheng; Zhang, Chulong

doi:10.1002/mbo3.666

Cited by 9 publications

(7 citation statements)

References 19 publications

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“…Earlier, naphthalene and sabinene monohydrates were reported by Daisy et al (2002) and Singh et al (2011) from Muscodor vitigenus and Phomopsis sp., respectively. The endophytic VOCs are majorly found to be bio-active, and our experimental outcomes support the same (Banerjee et al, 2010a,b;Kudalkar et al, 2012;Mao et al, 2019;Pena et al, 2019).…”

Section: Volatile Organic Compound-mediated Anti-fungal Actionsupporting

confidence: 89%

Broad-Spectrum Antimicrobial Action of Cell-Free Culture Extracts and Volatile Organic Compounds Produced by Endophytic Fungi Curvularia Eragrostidis

Santra

Banerjee

2022

Front. Microbiol.

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Endophytes are the mutualistic microorganisms that reside within the host plant and promote plant growth in adverse conditions. Plants and their endophytes are engaged in a symbiotic relationship that enables endophytes to access bioactive genes of the ethnomedicinal plants, and, as a result, endophytes are constantly addressed in the sector of pharmaceuticals and agriculture for their multidomain bio-utility. The gradual increase of antimicrobial resistance can be effectively countered by the endophytic metabolites. In these circumstances, in the present investigation, endophytic Curvularia eragrostidis HelS1 was isolated from an ethnomedicinally valuable plant Helecteris isora from East India’s forests. The secondary volatile and non-volatile metabolites are extracted from HelS1 and are found to be effective broad-spectrum antimicrobials. A total of 26 secondary metabolites (9 volatiles and 17 non-volatiles) are extracted from the isolate, which exhibits effective antibacterial [against six Gram-positive and seven Gram-negative pathogens with a minimum inhibitory concentrations (MIC) value ranging from 12.5 to 400 μg ml–1] and antifungal (against seven fungal plant pathogens) activity. The secondary metabolite production was optimised by one variable at a time technique coupled with the response surface methodology. The results revealed that there was a 34% increase in antibacterial activity in parameters with 6.87 g L–1 of fructose (as a carbon source), 3.79 g L–1 of peptone (as a nitrogen source), pH 6.75, and an inoculation period of 191.5 h for fermentation. The volatile metabolite production was also found to be optimum when the medium was supplemented with yeast extract and urea (0.2 g L–1) along with dextrose (40 g L–1). Amongst extracted volatile metabolites, 1-H-indene 1 methanol acetate, tetroquinone, N, N-diphenyl-2-nitro-thio benzamide, Trans 1, 2-diethyl-trans-2-decalinol, naphthalene, and azulene are found to be the most effective. Our investigation opens up opportunities in the sector of sustainable agriculture as well as the discovery of novel antimicrobials against dreadful phyto and human pathogens.

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Section: Volatile Organic Compound-mediated Anti-fungal Actionsupporting

confidence: 89%

Broad-Spectrum Antimicrobial Action of Cell-Free Culture Extracts and Volatile Organic Compounds Produced by Endophytic Fungi Curvularia Eragrostidis

Santra

Banerjee

2022

Front. Microbiol.

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“…However, the isolation method was mainly considered the ability of the isolated strains to produce diffusible antifungal compounds. Recent studies have demonstrated that VOCs as well as the diffusible antifungal compounds produced by PGPR have equally important functions in antifungal activities (Mao, Chen, & Xia, ; Ossowicki, Jafra, & Garbeva, ; Xie, Zang, & Wu, ). In this study, the dual‐culture and double‐plate chamber method were introduced to distinguish the ability of the isolated strains to produce diffusible and volatile antifungal compounds.…”

Section: Discussionmentioning

confidence: 99%

Antifungal and plant growth promotion activity of volatile organic compounds produced by Bacillus amyloliquefaciens

Zhou

et al. 2019

MicrobiologyOpen

131

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Fusarium wilt of watermelon, caused by F. oxysporum f.sp. niveum (FON), is a devastating disease that causes extensive losses throughout the world. Five bacterial strains (L3, h, β, b, and L) isolated from the watermelon rhizosphere showed antagonistic activity against FON during in vitro tests. Strain L3 produced diffusible and volatile organic compounds (VOCs) which showed the strongest antifungal activity. Arabidopsis thaliana plantlets exposed to VOCs produced by strain L3 showed a 2.39‐fold increase in biomass, 1.40‐fold increase in primary root length, and 5.05‐fold increase in number of lateral roots. Confocal laser scanning microscope showed that the GFP‐labeled strain L3 could colonize along the elongation and differentiation zones of watermelon roots. In greenhouse pot experiments, the biocontrol efficiency of strain L3 against fusarium wilt of watermelon was up to 68.4% in comparison with the control treatment. In addition, inoculation of the strain L3 resulted in a 23.4% increase in plant fresh weight. Based on 16S rDNA sequence analysis, the strain L3 was identified as Bacillus amyloliquefaciens L3. Fourteen VOCs produced by strain L3 were identified through GC‐MS analysis. Of nine VOCs tested, 2‐nonanone and 2‐heptanone were proved to have strong antifungal properties. Acetoin and 2,3‐butanediol were found to promote plant growth. The results suggested B. amyloliquefaciens L3 was a potential biocontrol agent, and that VOCs produced by B. amyloliquefaciens L3 play important roles in the process of biocontrol and plant growth promotion.

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“…The morphological characteristics of Induratia species were based on colony and mycelial characteristics, as reproductive structures have never been observed in any culture medium (Strobel et al 2001;Zhang et al 2010;Chen et al 2019;Mao et al 2018). However, Samarakoon et al (2020) found the sexual state of two specimens from Thailand, I. ziziphi and I. thailandica.…”

Section: Discussionmentioning

confidence: 99%

Polyphasic characterization and antimicrobial properties of Induratia species isolated from Coffea arabica in Brazil

et al. 2021

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Fungi belonging to the genus Induratia are endophytes that have received considerable attention because of the production of natural bioactive secondary metabolites, such as volatile compounds, with antimicrobial activity. In this study, we distinguished I. coffeana and Induratia sp. isolated from Coffea arabica in Brazil based on three-loci phylogeny, ITS, RPB2, and TUB2. The Induratia isolates showed high morphological plasticity and produced different volatile organic compounds, indicating that these compounds might not be correlated with their phylogenetic assignment. However, PLS-DA was able to discriminate Induratia isolates into three different clusters: one associated with I. coffeana and two with Induratia sp. Induratia isolates showed biofumigant activity against Botrytis cinerea with emphasis on I. coffeana (CML 4019), which inhibited the pathogenic fungus in postharvest strawberries. Nematicidal activity against Meloidogyne incognita was also observed in filtrates and volatile compounds produced by Induratia isolates. Moreover, we observed that I. coffeana (CML 4019) metabolites showed antibacterial activity against Staphylococcus aureus, Enterococcus faecalis, and E. faecium and that extracts of seven other Induratia isolates reduced the pre-formed biofilm of Staphylococcus aureus and Staphylococcus epidermidis.

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Identification and characterization of new Muscodor endophytes from gramineous plants in Xishuangbanna, China

Abstract: The endophytic fungi Muscodor spp. produce volatile organic compounds (VOCs) which can inhibit and even kill pathogenic fungi, bacteria, and nematodes. Nine endophytic fungal strains, isolated from the shoots of gramineous plants including

Cited by 9 publications

References 19 publications

Broad-Spectrum Antimicrobial Action of Cell-Free Culture Extracts and Volatile Organic Compounds Produced by Endophytic Fungi Curvularia Eragrostidis

Broad-Spectrum Antimicrobial Action of Cell-Free Culture Extracts and Volatile Organic Compounds Produced by Endophytic Fungi Curvularia Eragrostidis

Antifungal and plant growth promotion activity of volatile organic compounds produced by Bacillus amyloliquefaciens

Polyphasic characterization and antimicrobial properties of Induratia species isolated from Coffea arabica in Brazil

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