Identification and characterization of neutrophil extracellular trap shapes in flow cytometry

Ginley, Brandon; Emmons, Tiffany R.; Sasankan, Prabhu; Urban, Constantin F.; Segal, Brahm H.; Sarder, Pinaki

doi:10.1117/12.2254680

Cited by 2 publications

(2 citation statements)

References 8 publications

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“…Neural network research focuses on infectious diseases and autoimmune diseases, but it is also closely related to thrombotic diseases (acute myocardial infarction). 17,18 NETs are a newly discovered neutrophil bactericidal mechanism that activates neutrophils to release chromatin and then combine with the gingival sulcus to form a new defence network against gingival plaque bacteria. 19 It has been reported 20,21 that under the induction of IL-8, LPS and interferon, the neutrophil nucleus is deformed, the chromatin is homogeneous, the nuclear membrane is broken, the nuclear material is directly in contact with the particles, forming a mixture, and finally the cell membrane is broken and NETs are released.…”

Section: Discussionmentioning

confidence: 99%

Characteristics of neutrophil extracellular traps in patients with periodontitis and gingivitis

2020

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We sought to compare the characteristics and clinical significance of neutrophil extracellular traps in gingival samples from patients with periodontitis and those with gingivitis. The clinical indexes of gingival samples from patients with periodontitis and gingivitis were measured; the expression of TNF-alpha and IL-8 was measured by real-time fluorescent quantitative PCR; and the expression of TLR-8 and MMP-9 was measured by western blotting assays. Chemotaxis, phagocytosis and phagocytic activity of neutrophils were measured. Compared with the healthy group, the expression of TNF-α and IL-8 in the periodontitis group and the gingivitis group increased significantly (p < 0.05), and TNF-α in the gingivitis group was significantly lower than that in the healthy group (p < 0.05). The expression of IL-8 in the periodontitis group was significantly higher than that in the periodontitis group (p < 0.05). Furthermore, the expression of TLR-8 and MMP-9 in the periodontitis group was different from that in the gingivitis group and the healthy group, and the expression of TLR-8 and MMP-9 in the gingivitis group was significantly different from that in the healthy group (p < 0.05). In addition, the neutrophil mobility index in healthy people was 3.02 ± 0.53, that in the periodontitis group was 2.21 ± 0.13, and that in the gingivitis group was 2.31 ± 0.12. In conclusion, the chemotaxis of neutrophils in gingival samples of patients with periodontitis and gingivitis was decreased, the phagocytotic ability and activity of neutrophils were reduced, and the release of the extracellular trap-releasing inducible factors TNF-alpha and IL-8 also declined.

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Section: Discussionmentioning

confidence: 99%

Characteristics of neutrophil extracellular traps in patients with periodontitis and gingivitis

2020

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“…If the removal is not timely, a large amount of antigens will accumulate, which may induce the body to produce a large amount of autoantibodies, leading to autoimmune diseases. Neural network research focuses on infectious diseases and autoimmune diseases, but it is also closely related to thrombotic diseases (acute myocardial infarction) (16).…”

Section: Discussionmentioning

confidence: 99%

A comparative study of the characteristics and clinical significance of extracellular traps of neutrophils in gingival samples of periodontitis and gingivitis patients

Zhang

Yang

Song

2019

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Background: We sought to compare the characteristics and clinical significance of extracellular traps of neutrophils in gingival samples of periodontitis and gingivitis. Methods: The clinical indexes of gingival samples from periodontitis and gingivitis were measured; the expression of TNF-alpha and IL-8 were measured by real-time fluorescent quantitative PCR; the expression of TLR-8 and MMP-9 were measured by Western blotting assays Chemotaxis, phagocytosis and phagocytic activity of neutrophils were measured. Results: The periodontal clinical indexes of periodontitis and gingivitis samples were significantly higher than those of healthy people (P<0.05). There were significant differences in BOP, PD and CAL between periodontitis and gingivitis samples (P<0.05), but no differences in gingival index (GI) (P>0.05). Compared with the healthy group, the expressions of TNF-α and IL-8 in the periodontitis group and the gingivitis group increased significantly (P<0.05), and TNF-α in the gingivitis group was significantly lower than that in the healthy group (P<0.05). The expression of IL-8 in the periodontitis group was significantly higher than that in the periodontitis group (P<0.05); the expression of TLR-8 and MMP-9 in the periodontitis group was different from that in the gingivitis group and the healthy people group, and the expression of TLR-8 and MMP-9 in the gingivitis group was significantly different from that in the healthy people group (P<0.05); the neutrophil mobility index in healthy people was (3.02±0.53), and that in the periodontitis group was (2.21±0.13), and (2.31±0.12) in the gingiva. There were significant differences between the periodontitis group and the gingivitis group (P<0.05, P<0.05). The phagocytic ability and activity of neutrophils in healthy people were (392.03±20.04), (89.34±10.12), the periodontitis group was (360.32±18.23), (80.02±10.21), and the gingivitis group was (359.32±20.11), (78.93±17.32), respectively. There were significant differences between the periodontitis group and the gingivitis group and healthy people group (P<0.05, P<0.05). Conclusion: The chemotaxis of neutrophils in gingival samples of periodontitis and gingivitis patients was decreased, the phagocytosis ability and activity of neutrophils were reduced, and the release of extracellular trap-releasing inducible factors TNF-alpha and IL-8 also declined.

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Identification and characterization of neutrophil extracellular trap shapes in flow cytometry

Cited by 2 publications

References 8 publications

Characteristics of neutrophil extracellular traps in patients with periodontitis and gingivitis

Characteristics of neutrophil extracellular traps in patients with periodontitis and gingivitis

A comparative study of the characteristics and clinical significance of extracellular traps of neutrophils in gingival samples of periodontitis and gingivitis patients

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