Identification and characterization of mkk genes and their expression profiles in rainbow trout (Oncorhynchus mykiss) symptomatically or asymptomatically infected with Vibrio anguillarum

Yang, Xiaodong; Hou, Zhi-Shuai; Liu, Meng-Qun; Zeng, Chu; Zhao, Hong-Kui; Xin, Yuan-Ru; Xiang, Kai-Wen; Yang, Qian; Wen, Haishen; Li, Jifang

doi:10.1016/j.fsi.2021.12.049

Cited by 6 publications

(2 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The correlation analysis of gene expressions was analyzed via the Pearson correlation coefficient by MetaboAnalyst and GraphPad Prism 8.0. Data of basal expression of ghrh-gh-sst-igf axis in diploid trout were retrieved from previously published RNA-Seq data (SRA of NCBI: PRJNA865462 for liver; SRA of NCBI: PRJNA753277 for brain and kidney) [ 69 , 70 ]. The gene count data were analyzed by DESeq2 R package and then the average count was normalized by average β-actin ((average of normalized count × 10,000)/(average of β-actin count)) [ 60 ].…”

Section: Methodsmentioning

confidence: 99%

Crosstalk between Growth and Osmoregulation of GHRH-SST-GH-IGF Axis in Triploid Rainbow Trout (Oncorhynchus mykiss)

Xiang

Yang

Liu

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

Smolting is an important development stage of salmonid, and an energy trade-off occurs between osmotic regulation and growth during smolting in rainbow trout (Oncorhynchus mykiss). Growth hormone releasing hormone, somatostatin, growth hormone and insulin-like growth factor (GHRH-SST-GH-IGF) axis exhibit pleiotropic effects in regulating growth and osmotic adaptation. Due to salmonid specific genome duplication, increased paralogs are identified in the ghrh-sst-gh-igf axis, however, their physiology in modulating osmoregulation has yet to be investigated. In this study, seven sst genes (sst1a, sst1b, sst2, sst3a, sst3b, sst5, sst6) were identified in trout. We further investigated the ghrh-sst-gh-igf axis of diploid and triploid trout in response to seawater challenge. Kidney sst (sst1b, sst2, sst5) and sstr (sstr1b1, sstr5a, sstr5b) expressions were changed (more than 2-fold increase (except for sstr5a with 1.99-fold increase) or less than 0.5-fold decrease) due to osmoregulation, suggesting a pleiotropic physiology of SSTs in modulating growth and smoltification. Triploid trout showed significantly down-regulated brain sstr1b1 and igfbp2a1 (p < 0.05), while diploid trout showed up-regulated brain igfbp1a1 (~2.61-fold, p = 0.057) and igfbp2a subtypes (~1.38-fold, p < 0.05), suggesting triploid trout exhibited a better acclimation to the seawater environment. The triploid trout showed up-regulated kidney igfbp5a subtypes (~6.62 and 7.25-fold, p = 0.099 and 0.078) and significantly down-regulated igfbp5b2 (~0.37-fold, p < 0.05), showing a conserved physiology of teleost IGFBP5a in regulating osmoregulation. The IGFBP6 subtypes are involved in energy and nutritional regulation. Distinctive igfbp6 subtypes patterns (p < 0.05) potentially indicated trout triggered energy redistribution in brain and kidney during osmoregulatory regulation. In conclusion, we showed that the GHRH-SST-GH-IGF axis exhibited pleiotropic effects in regulating growth and osmoregulatory regulation during trout smolting, which might provide new insights into seawater aquaculture of salmonid species.

show abstract

Section: Methodsmentioning

confidence: 99%

Crosstalk between Growth and Osmoregulation of GHRH-SST-GH-IGF Axis in Triploid Rainbow Trout (Oncorhynchus mykiss)

Xiang

Yang

Liu

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…Upon stimulation by growth factors, inflammation, hormones, as well as endogenous stress and environmental signals, MAPKs receive activation signals from MKKs and MKKKs, are activated in response, and manifest as cascading phosphorylation. 18 MAPKs are traditionally classified into 2 major categories: mitogenic and stress-activated MAPKs. The classical representatives of mitogenic responses are ERK MAPKs which have notable effects in tumor multiplication, transfer, and aggression; tumor extracellular matrix degradation; and tumor angiogenesis.…”

Section: Introductionmentioning

confidence: 99%

PKC-δ Promotes IL-1β-Induced Apoptosis of Rat Chondrocytes and Via Activating JNK and P38 MAPK Pathways

Lü

2023

CARTILAGE

View full text Add to dashboard Cite

Objective Protein kinase C-delta (PKC-δ) is involved in apoptosis. This study aimed to establish whether PKC-δ can further promote IL-1β-induced chondrocyte apoptosis by mediating the phosphorylation of the JNK and p38 mitogen-activated protein kinase (MAPK) signaling pathways In osteoarthritis (OA). Methods We employed chondrocyte staining to determine the extent of cartilage degeneration. PKC-δ and p38 signal expressions were used in the immunohistochemical (IHC) test and apoptosis was assayed at the TUNEL test in human osteoarthritic and controls. We stimulated rat cartilage cells using IL-1β (10 ng/ml)/rottlerin (10 μM) or lentivirus. To determine the apoptosis rate, we employed flow cytometry. The mRNA of both BCL2-related X (BAX) and cysteine aspartate protease 3 (caspase-3) could be measured via qRT-PCR. Western blot measured the protein levels of BAX, caspase-3, PKC-δ, p-JNK/JNK and p-p38/p38. Results The positive rate of PKC-δ and the apoptotic rate of chondrocytes in OA were higher than controls. The manifestation of PKC-δ was positively related to the degree of cartilage degeneration, p38 protein expression, and apoptosis rate. IL-1β exposure upregulated PKC-δ expression in chondrocytes in a dose-dependent manner. Decreasing PKC-δ expression and its phosphorylation in OA can inhibit MAPK signaling pathway activation (phosphorylation) by downregulating JNK and p38 protein phosphorylation and expression. This inhibition decreases caspase-3 and BAX levels, consequently lowering the apoptosis rate in chondrocytes. Conclusion PKC-δ activation by IL-1β in OA promotes chondrocyte apoptosis via activation of the JNK and p38 MAPK signal pathways, thereby promoting the OA progression.

show abstract

Genome-wide identification of MKK and MAPK gene families and their expression analysis under abiotic stress in largemouth bass (Micropterus salmoides)

Chen

et al. 2022

Aquaculture

View full text Add to dashboard Cite

Identification and characterization of mkk genes and their expression profiles in rainbow trout (Oncorhynchus mykiss) symptomatically or asymptomatically infected with Vibrio anguillarum

Cited by 6 publications

References 73 publications

Crosstalk between Growth and Osmoregulation of GHRH-SST-GH-IGF Axis in Triploid Rainbow Trout (Oncorhynchus mykiss)

Crosstalk between Growth and Osmoregulation of GHRH-SST-GH-IGF Axis in Triploid Rainbow Trout (Oncorhynchus mykiss)

PKC-δ Promotes IL-1β-Induced Apoptosis of Rat Chondrocytes and Via Activating JNK and P38 MAPK Pathways

Genome-wide identification of MKK and MAPK gene families and their expression analysis under abiotic stress in largemouth bass (Micropterus salmoides)

Contact Info

Product

Resources

About