Identification and characterization of microsatellite markers in the Chagas disease vector Triatoma dimidiata

Anderson, Jennifer M.; Lai, James E.; Dotson, Ellen M.; Cordón-Rosales, Celia; Ponce, Carlos; Norris, Douglas E.; Beard, Charles B.

doi:10.1016/s1567-1348(02)00033-3

Cited by 26 publications

(21 citation statements)

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“…The sequences flanking the loci were not similar nor did the primer sequences match sufficiently to amplify. Thus, we consider our microsatellite loci different from the published sequences for R. pallescens (Harry et al, 1988), T. dimidiata (Anderson et al, 2002) and T. infestans (García et al, 2004).…”

Section: Resultsmentioning

confidence: 99%

“…Microsatellite loci have been previously described for triatomine Rhodnius pallescens (Harry et al, 1988), Triatoma dimidiata (Anderson et al, 2002) and very recently for T. infestans (García et al, 2004), but thus far, no works applying or testing those markers for population genetics studies have been published. The present work not only describes but also tests the suitable of new microsatellite loci for T. infestans for population genetic studies as part of a comprehensive study on the eco-epidemiology and control of Chagas disease in northern Argentina.…”

Section: Introductionmentioning

confidence: 99%

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Identification and characterization of microsatellite markers in the Chagas disease vector Triatoma infestans (Heteroptera: Reduviidae)

Marcet

Lehmann

Groner

et al. 2006

Infection, Genetics and Evolution

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Triatoma infestans, the main vector of Chagas disease in the southern cone countries, is the principal target of a regional elimination program. A better understanding of its dispersal, sources of reinfestation, and insecticide resistance is key to an effective control program. To address such problems, we identified and characterized 13 microsatellite loci of T. infestans. For each locus, primer sequences and PCR conditions are presented. Allele variability and frequency were analyzed in 59 T. infestans specimens from different rural communities in northwestern Argentina; nine loci were considered suitable for population genetic studies. Departure from Hardy-Weinberg equilibrium was detected in 10/13 loci with F IS values ranging from 0.04 to 0.91, indicating heterozygote deficit and a possible grade of sub-structure in the sample analyzed. Presence of null alleles in some loci cannot be discarded. The present work provides a promising tool to develop a population genetic study of natural populations of T. infestans in tandem with field studies and analyses of bug dispersal and the reinfestation process.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification and characterization of microsatellite markers in the Chagas disease vector Triatoma infestans (Heteroptera: Reduviidae)

Marcet

Lehmann

Groner

et al. 2006

Infection, Genetics and Evolution

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“…Several DNAbased techniques have been hitherto applied to triatomines Lyman 1999, Monteiro et al 2001). These include randomly amplified polymorphic DNA (RAPD) (Carlier et al 1996, Dujardin et al 1998a, Noireau et al 2000a, Borges et al 2000, Jaramillo et al 2001, Pacheco et al 2003, species-specific length variation of rDNA amplicons (Jaramillo et al 2001), single strand conformational polymorphism analysis , sequencing of selected nuclear (Monteiro et al 2000, 2001 and mitochondrial gene fragments (García and Powell 1998, García et al 2001, Gaunt and Miles 2002, Hypša et al 2002, Sainz et al 2004, and characterization of polymorphic microsatellite markers (Harry et al 1998, Anderson et al 2002). …”

Section: Dna-based Methodsmentioning

confidence: 99%

Molecular research and the control of Chagas disease vectors

Abad‐Franch

Monteiro²

2005

An. Acad. Bras. Ciênc.

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Chagas disease control initiatives are yielding promising results. Molecular research has helped successful programs by identifying and characterizing introduced vector populations and by defining intervention targets accurately. However, researchers and health officials are facing new challenges throughout Latin America. Native vectors persistently reinfest insecticide-treated households, and sylvatic triatomines maintain disease transmission in humid forest regions (including Amazonia) without colonizing human dwellings. In these scenarios, fine-scale vector studies are essential to define epidemiological risk patterns and clarify the involvement of little-known triatomine taxa in disease transmission. These eco-epidemiological investigations, as well as the planning and monitoring of control interventions, rely by necessity on accurate taxonomic judgments. The problems of cryptic speciation and phenotypic plasticity illustrate this need -and how molecular systematics can provide the fitting answers. Molecular data analyses also illuminate basic aspects of vector evolution and adaptive trends. Here we review the applications of molecular markers (concentrating on allozymes and DNA sequencing) to the study of triatomines. We analyze the suitability, strengths and weaknesses of the various techniques for taxonomic, systematic and evolutionary investigations at different levels (populations, species, and higher taxonomic categories).Key words: molecular systematics, DNA, allozymes, Triatominae, Chagas disease. CHAGAS DISEASE: BURDEN, CONTROL EFFORTS AND NEW CHALLENGESChagas disease (CD), caused by Trypanosoma cruzi and transmitted by triatomine bugs, is a major public health problem in Latin America. An overall prevalence of ∼ 18 million infections was estimated in the late 1980s, with 90 million people living at risk and 45000 deaths/year. CD was the most important parasitic disease in Latin America in terms of its impact on national economies and public health systems (WHO 1991, World Bank 1993.In the absence of vaccines or adequate drugs for large-scale treatment, the reduction of disease burden critically depends on the control of transmission by triatomine vectors and infected blood transfusion. Several multinational initiatives have been launchedAn Acad Bras Cienc (2005) 77 (3) 438FERNANDO ABAD-FRANCH and FERNANDO A. MONTEIRO with that purpose. Ten years of concerted action in the Southern Cone have resulted in the elimination of transmission by Triatoma infestans (the most widespread domestic vector) from vast areas of the region. Incidence dropped by an average of 94% in the area, and by 65% in Latin America (WHO 2002). Recent estimates show however that substantial efforts are still necessary: overall prevalence remains over 12 million, with 200000 new cases/year in 15 countries (Morel and Lazdins 2003).The success of the Southern Cone initiative was eased by the fact that the main vector species, T. infestans, is entirely domestic throughout most of its range, making reinfestation of insectic...

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“…Já existem caracterizados e descritos primers para R. pallescens (10 loci), R. ecuadoriensis (6 loci), R. prolixus (20 loci), R. robustus (10 loci), T. brasiliensis (6 loci), T. dimidiata (8 loci), T. infestans (19 loci) e T. pseudomaculata (6 loci;Harry et al 1998, Anderson et al 2002, García et al 2004, Marcet et al 2006, Fitzpatrick et al 2009). …”

Section: Microssatélitesunclassified

Vetores da doença de Chagas no Brasil

Galvão¹

2014

105

107

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Identification and characterization of microsatellite markers in the Chagas disease vector Triatoma dimidiata

Cited by 26 publications

References 21 publications

Identification and characterization of microsatellite markers in the Chagas disease vector Triatoma infestans (Heteroptera: Reduviidae)

Identification and characterization of microsatellite markers in the Chagas disease vector Triatoma infestans (Heteroptera: Reduviidae)

Molecular research and the control of Chagas disease vectors

Vetores da doença de Chagas no Brasil

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