Identification and characterization of microRNAs involved in double-color formation in Paeonia suffruticosa ‘Shima Nishiki’ by high-throughput sequencing

Zhang, Xinpeng; Jia, Jinshan; Zhao, Mingyuan; Li, Cheng; Han, Xu; Xu, Zongda

doi:10.1007/s13580-021-00379-2

Cited by 3 publications

(5 citation statements)

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“…With the rapid development of next-generation high-throughput sequencing technology, many researchers are studying the coloring molecular mechanism of double-color flowers in many ornamental plants based on sequencing technologies such as transcriptome, miRNA, proteome, and methylation. These plants mainly include peach (Chen et al, 2014), lotus (Deng et al, 2022), lily (Xu et al, 2017), herbaceous peony (Zhao et al, 2014(Zhao et al, , 2017a, tree peony (Zhang et al, 2018a(Zhang et al, , 2020(Zhang et al, , 2022, phalaenopsis , pansy (Wang et al, 2022), and other ornamental plant species. HORTSCIENCE VOL.…”

Section: Discussionmentioning

confidence: 99%

“…Yamagishi and Sakai (2020) found that the specific expressions of miR828 and MYB12 in the miR828/MYB12 regulation module are closely related to the coloring of doublecolor flowers in five Asiatic hybrid lily bicolor cultivars. Zhang et al (2022) found that the specific expressions of miR858 and PsMYB12L in the miR858/PsMYB12L module may be one of the important reasons for regulating the color difference of its double-color flowers in P. suffruticosa 'Shima Nishiki'.…”

Section: Molecular Mechanism Affecting the Color Difference Of Double...mentioning

confidence: 99%

“…In recent years, studies in many plants have shown that some miRNAs (miR156, miR828, miR858, miR395, miR827, etc.) can directly affect their target genes (such as some TFs involved in the regulation of flavonoid biosynthesis), then affect the expression level of key structural genes related to flavonoid biosynthesis, and ultimately regulate flower color differences (Bonar et al, 2018;Gou et al, 2011;Li et al, 2020;Pashkovskiy et al, 2021;Tirumalai et al, 2019;Yamagishi and Sakai, 2020;Zhang et al, 2022). Zhao et al (2017aZhao et al ( , 2017b found that the specific expressions of miR156e-3p and SPL1 in the miR156e-3p/SPL1 regulation module may be an important reason for the yellow formation of double-color flowers in P. lactiflora 'Jinhui'.…”

Section: Molecular Mechanism Affecting the Color Difference Of Double...mentioning

confidence: 99%

“…The flowers of this chimera usually have a colorful and prominent phenotype and are often favored by many consumers in the flower market (Li and He, 2005;Noman et al, 2017;Wang et al, 2022;Wu et al, 2020). Therefore, it is not hard to understand that the research and cultivation of these unique double-color flowers has also become one of the main directions of many researchers and breeding workers Zhang et al, 2019Zhang et al, , 2022.…”

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Advances on the Coloring Mechanism of Double-color Flowers in Plants

Zhang

Wang

et al. 2022

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Plants with the flower color phenotype of double-color flowers are very precious and attractive and can usually be regarded as valuable germplasm resources for studying and improving flower color. This paper summarizes the coloring mechanism of double-color flowers in plants from three aspects: the formation of double-color flowers, the physiological factors affecting the coloring difference of double-color flowers, and the molecular mechanism affecting the coloring difference of double-color flowers, to provide a theoretical reference for the in-depth study of the coloring mechanism and molecular breeding of double-color flowers in the future.

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Section: Discussionmentioning

confidence: 99%

Section: Molecular Mechanism Affecting the Color Difference Of Double...mentioning

confidence: 99%

Section: Molecular Mechanism Affecting the Color Difference Of Double...mentioning

confidence: 99%

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confidence: 99%

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Advances on the Coloring Mechanism of Double-color Flowers in Plants

Zhang

Wang

et al. 2022

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“…The flower colors of tree peonies can be divided into nine types: red, pink, yellow, purple, green, white, black, blue, and multi-color. Most research on tree peonies has focused on red, pink, yellow, purple, and multi-color blooms [2][3][4][5][6][7][8][9] and petal blotch [10,11], which are bright and conspicuous, with no prior studies reported on green-colored peonies. There are more than ten cultivars of green flower peonies in China, but all their blooms transition to white or pink upon opening, significantly diminishing their ornamental appeal.…”

Section: Introductionmentioning

confidence: 99%

Selection of Stable Reference Genes for QRT-PCR in Tree Peony ‘Doulv’ and Functional Analysis of PsCUC3

Zhou,

Ma,

Zhou

et al. 2024

Plants

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(1) Background: Tree peonies display extensive cultivar diversity due to widespread hybridization, resulting in a complex genetic architecture. This complexity complicates the selection of universal reference genes across different cultivars for qRT-PCR analyses. Paeonia suffruticosa ‘Doulv’, notable for its unique green blooms in China, exhibits chlorosis post-flowering and features petaloid stamens and pistils. (2) Methods: Based on published literature and RNA-seq data from ‘Doulv’, nine candidate reference genes—ACT (Actin), TUB (β-Tubulin), UBC (Ubiquitin Conjugating Enzyme), UBQ (Ubiquitin), UPL (Ubiquitin Protein Ligase), PP2A (Protein Phosphatase 2A), PP2C (Protein Phosphatase 2C), MBF1A (Multiprotein Bridging Factor 1A), and GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase)—were selected. Their expression stability was assessed across various tissues and developmental stages of ‘Doulv’ flowers using qRT-PCR, with evaluations conducted via GeNorm_v3.5, NormFinder_v20, and BestKeeper_v1.0. Gene cloning and expression analyses of PsCUC3, including its subcellular localization, were performed. (3) Results: GAPDH and ACT were identified as the most stable reference genes in petaloid stamens across various developmental stages of ‘Doulv’, whereas UBC and MBF1A were optimal across different tissues. Notably, specific conserved amino acids in PsCUC3 from ‘Doulv’ diverged from those in NAM/CUC3 proteins of other species, impacting its protein structure. PsCUC3 expression analysis revealed no correlation with chlorophyll content in petaloid stamens but an association with petaloid organ development. Furthermore, PsCUC3 was predominantly localized in the nucleus. (4) Conclusions: This study comprehensively evaluated suitable reference genes using GeNorm_v3.5, NormFinder_v20, and BestKeeper_v1.0 software, establishing a robust qRT-PCR detection system for ‘Doulv’ peony. These results provide a solid experimental foundation for further research on ‘Doulv’ peony. Building on this experimental foundation, the functional analysis of the PsCUC3 gene was conducted. The findings suggest a potential association between the PsCUC3 gene and floral morphology alterations in ‘Doulv’, identifying PsCUC3 as crucial for understanding the molecular mechanisms influencing floral structure in tree peonies.

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