Identification and characterization of microRNAs in the gonad of Trachinotus ovatus using Solexa sequencing

He, Pingping; Wei, Pinyuan; Chen, Xiaohan; Lin, Yong; Peng, J.

doi:10.1016/j.cbd.2019.03.010

Cited by 13 publications

(18 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a critical regulator of gene expression, let-7 miRNAs are abundant in gonadal tissues of mammals and are involved in multiple physiological processes, such as the regulation of sexual maturity, sperm formation and oocyte maturation 40 – 42 . In teleosts, the let-7 family was also highly expressed in the gonads of Trachinotus ovatus 34 , C. carpio 22 , P. olivaceus 23 , Megalobrama amblycephala 43 , Danio rerio 35 , and Oryzias latipes 25 , consistent with the expression in T. rubripes , indicating functional conservation across vertebrates and suggesting that the let-7 family might be crucial for reproductive physiology. In P. olivaceus , female-biased expression of miR-200b was found, and the expression level in the testes was sevenfold lower than in the ovaries 23 .…”

Section: Discussionsupporting

confidence: 56%

See 1 more Smart Citation

Identification of sex differentiation-related microRNA and long non-coding RNA in Takifugu rubripes gonads

Yan

Liu

Jiang

et al. 2021

Sci Rep

View full text Add to dashboard Cite

Although sex determination and differentiation are key developmental processes in animals, the involvement of non-coding RNA in the regulation of this process is still not clarified. The tiger pufferfish (Takifugu rubripes) is one of the most economically important marine cultured species in Asia, but analyses of miRNA and long non-coding RNA (lncRNA) at early sex differentiation stages have not been conducted yet. In our study, high-throughput sequencing technology was used to sequence transcriptome libraries from undifferentiated gonads of T. rubripes. In total, 231 (107 conserved, and 124 novel) miRNAs were obtained, while 2774 (523 conserved, and 2251 novel) lncRNAs were identified. Of these, several miRNAs and lncRNAs were predicted to be the regulators of the expression of sex-related genes (including fru-miR-15b/foxl2, novel-167, novel-318, and novel-538/dmrt1, novel-548/amh, lnc_000338, lnc_000690, lnc_000370, XLOC_021951, and XR_965485.1/gsdf). Analysis of differentially expressed miRNAs and lncRNAs showed that three mature miRNAs up-regulated and five mature miRNAs were down-regulated in male gonads compared to female gonads, while 79 lncRNAs were up-regulated and 51 were down-regulated. These findings could highlight a group of interesting miRNAs and lncRNAs for future studies and may reveal new insights into the function of miRNAs and lncRNAs in sex determination and differentiation.

show abstract

Section: Discussionsupporting

confidence: 56%

“…miR-100 is also abundant in the undifferentiated gonads of O. niloticus 19 , and in the mature ovaries and testes of Pelteobagrus fulvidraco , and H. hippoglossus 14 , 17 . In T. ovatus , the miR-30 family is highly expressed in the testes 34 . In O. niloticus 18 and D. rerio 35 , the miR-181a family is also abundantly expressed.…”

Section: Discussionmentioning

confidence: 99%

Identification of sex differentiation-related microRNA and long non-coding RNA in Takifugu rubripes gonads

Yan

Liu

Jiang

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…To better compare the similarities and differences of the miRNA expression profiles in Acanthopagrus latus and six reported fishes. Except for the species-specific miRNAs, we summarized nine testis- or ovary-biased miRNAs, including let-7, miR-10, miR-7, miR-9, miR-202, miR-200, miR-29, miR-21, and miR-725, which exhibited significant sex-biased expression in at least four fish species ( Table 2 ) [ 18 , 19 , 20 , 21 , 22 , 25 , 26 , 27 ]. MiR-7 exhibited a significant testis-biased expression in Acanthopagrus latus and three other fish species, indicating that it might play an important role in testis development.…”

Section: Discussionmentioning

confidence: 99%

“…In mice, miR-143 regulates granulosa cell proliferation by targeting the FSH signaling pathway [ 46 ]. The miR-30 family was highly expressed in the testes of Trachinotus ovatus [ 26 ], yellow catfish [ 19 ], and Amur sturgeon [ 19 , 25 ]. The miR-181 family is highly expressed in the gonads of tilapia [ 21 ], mice [ 47 ], and humans [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, some miRNAs, including miR-34c-5p, miR-153-5p, and miR-749, were expressed exclusively in Nile tilapia XX gonads at five days post-hatching, whereas miR-1306-5p, miR-132b, and miR-18c were expressed exclusively in the XY gonads [ 21 ]. MiRNA expression patterns have also been studied in the gonads of Oplegnathus punctatus [ 22 ], Scylla paramamosain [ 23 , 24 ], Acipenser schrenckii [ 25 ], Trachinotus ovatus [ 26 ], and Odontobutis potamophila [ 27 ]. These studies help to clarify the roles of miRNAs in sexual differentiation and gonadal development.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identification and Comparison of microRNAs in the Gonad of the Yellowfin Seabream (Acanthopagrus Latus)

Lin

Fang

et al. 2020

IJMS

View full text Add to dashboard Cite

Yellowfin seabream (Acanthopagrus latus) is a commercially important fish in Asian coastal waters. Although natural sex reversal has been described in yellowfin seabream, the mechanisms underlying sexual differentiation and gonadal development in this species remain unclear. MicroRNAs (miRNAs) have been shown to play crucial roles in gametogenesis and gonadal development. Here, two libraries of small RNAs, constructed from the testes and ovaries of yellowfin seabream, were sequenced. Across both gonads, we identified 324 conserved miRNAs and 92 novel miRNAs: 67 ovary-biased miRNAs, including the miR-200 families, the miR-29 families, miR-21, and miR-725; and 88 testis-biased miRNAs, including the let-7 families, the miR-10 families, miR-7, miR-9, and miR-202-3p. GO (Gene Ontology) annotations and KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analyses of putative target genes indicated that many target genes were significantly enriched in the steroid biosynthesis pathway and in the reproductive process. Our integrated miRNA-mRNA analysis demonstrated a putative negatively correlated expression pattern in yellowfin seabream gonads. This study profiled the expression patterns of sex-biased miRNAs in yellowfin seabream gonads, and provided important molecular resources that will help to clarify the miRNA-mediated post-transcriptional regulation of sexual differentiation and gonadal development in this species.

show abstract

Novel miRNA identification and comparative profiling of miRNA regulations revealed important pathways in Jinding duck ovaries by small RNA sequencing

et al. 2020

View full text Add to dashboard Cite

Functional studies have revealed miRNAs play pivotal roles in ovulation and ovary development in mammalians, whereas little is known about the miRNA function in ducks. In this study, miRNA deep sequencing in the ovary tissues was carried out to obtain the miRNA profile from ovaries before oviposition (BO) and after oviposition (AO) in Jinding duck. Overall, an average of 23,128,075 and 26,020,523 reads were identified in the BO and AO samples, respectively, and 6739 miRNAs were identified from them through further mapping and analysis. Besides, 1570 miRNAs were identified as differentially expressed miRNAs compared with BO, including 493 miRNAs up-regulated and 1077 down-regulated in AO. Moreover, 2291 target genes were predicted from 443 significantly differentially expressed miRNAs. In addition, GO and KEGG pathway analysis indicated that target genes were enriched in some basic cell metabolism pathways as well as the productive pathways such as MAPK signaling pathway, gonadotropin-releasing hormone signaling pathway, TGF-beta signaling pathway which had been significantly changed. Our results helped to replenish the duck miRNA database and illustrate the potential mechanism of miRNA function in duck ovary development and reproduction process.

show abstract

Identification and characterization of microRNAs in the gonad of Trachinotus ovatus using Solexa sequencing

Cited by 13 publications

References 42 publications

Identification of sex differentiation-related microRNA and long non-coding RNA in Takifugu rubripes gonads

Identification of sex differentiation-related microRNA and long non-coding RNA in Takifugu rubripes gonads

Identification and Comparison of microRNAs in the Gonad of the Yellowfin Seabream (Acanthopagrus Latus)

Novel miRNA identification and comparative profiling of miRNA regulations revealed important pathways in Jinding duck ovaries by small RNA sequencing

Contact Info

Product

Resources

About