Identification and characterization of microRNAs in white and black coated yak

Abstract

In this study, we used high-throughput technology to provide the first transcriptome dataset for differentially expressed miRNA in mixed pools of dermis tissue from black- and white-coated yak to research the possible molecular mechanisms of yak coat pigmentation. In this study, 92,636,002 and 95,917,842 clear reads were generated through Illumina paired-end sequencing. A total of 78 differentially expressed miRNAs (DEMs) were identified, including 59 upregulated and 19 downregulated miRNAs in the mixed pools of white-coated yak compared with the mixed pools of black-coated yak. In addition, 3634 genes were predicted as putative targets of DEMs. These DEGs related to 59 GO categories and were enriched in 216 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, including melanogenesis and the Wnt signaling pathway. The results of the current study indicated that the coat color of the yak involved the transcriptional regulation process of miRNAs. These results provide helpful data to understand the molecular mechanisms of yak coat pigmentation.

Differentially Expressed miRNAs in Mouse Uterus during Embryo Implantation

“…MiRNAs regulate more than 30% of chromosomes in the human genome (Bartel, 2004). The research on miRNA has gradually expanded from human and model animals to other common livestock (Basang et al, 2018), new miRNAs were often reported (Chang et al, 2018). As one of the most important regulators, miRNA can affect cell proliferation, apoptosis, adhesion, angiogenesis and migration directly or indirectly (Chen et al, 2016;Paramasivam et al, 2017) To achieve a mutually acceptable state between the maternal uterus and the embryo in the process of implantation, it is necessary to regulate many related genes to make the maternal body reach an optimally receptive state.…”