Identification and characterization of lymph organ microRNAs in red swamp crayfish, Procambarus clarkii infected with white spot syndrome virus

Du, Zhiqiang; Leng, Xiao-Yun; Shen, Xiu-li; Jin, Yanhui; Li, Xin-Cang

doi:10.1016/j.fsi.2017.08.007

Cited by 14 publications

(8 citation statements)

References 30 publications

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“…It thus seems miR-210 and miR-10b are typical bacterial induced miRNAs, as our KEGG analysis showed that the predicted target genes of the 12 potential AHPND-related miRNAs, were mainly enriched in the Vibrio cholerae subcategory. For miR-193, it had previously been reported to be associated with WSSV infection [ 35 ], and was shown to be upregulated upon low virulent WSSV infection [ 36 ]. While miR-210, miR-10b and miR-193 were shown in previous studies to be upregulated, their expression in this study was observed to be downregulated (VA relative to VN), suggesting that probably the expression of these miRNAs was suppressed so as to increase the expression or function of their associated target genes; a possible implication of their importance in shrimp immune defense.…”

Section: Discussionmentioning

confidence: 99%

Acute Hepatopancreatic Necrosis Disease (AHPND) related microRNAs in Litopenaeus vannamei infected with AHPND-causing strain of Vibrio parahemolyticus

et al. 2018

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BackgroundAcute hepatopancreatic necrosis disease (AHPND) has emerged as a major debilitating disease that causes massive shrimp death resulting in substantial economic losses in shrimp aquaculture. Given that several diseases and infections have been associated with microRNAs (miRNAs), we conducted a comparative transcriptomic analysis using the AHPND (VA) and non-AHPND (VN) strains of Vibrio parahemolyticus to identify miRNAs potentially involved in AHPND pathogenesis in Litopenaeus vannamei.ResultsA total of 83 miRNAs (47 upregulated and 36 downregulated) were significantly differentially expressed between the VA and VN challenged groups, while 222 target genes of these miRNAs were predicted. Functional enrichment analysis revealed that the miRNAs target genes were involved in multiple biological processes including metabolic pathways, amoebiasis, Vibrio cholerae infection etc. Finally, interaction network and qPCR (Real-time Quantitative PCR) analysis of 12 potential key AHPND-related miRNAs and their predicted target genes, revealed their possible involvement in modulating several immune-related processes in the pathogenesis of AHPND.ConclusionsWe have shown using comparative transcriptomic analysis, miRNAs and their target genes that are responsive to AHPND V. parahemolyticus infection in shrimp, therefore suggesting their possible role in defense response to AHPND V. parahemolyticus infection.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4728-4) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Acute Hepatopancreatic Necrosis Disease (AHPND) related microRNAs in Litopenaeus vannamei infected with AHPND-causing strain of Vibrio parahemolyticus

et al. 2018

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“…The red swamp crayfish, Procambarus clarkii , which is an invasive species in Europe and Asia, is also a highly adaptable aquaculture species that is gradually becoming an important aquatic food sector among fishery trading commodities in China ( Du et al, 2017 ). The pond monoculture and rice-crayfish cultivation models are the two main farming models used for crayfish in China.…”

Section: Introductionmentioning

confidence: 99%

Metagenomics Analysis Reveals Compositional and Functional Differences in the Gut Microbiota of Red Swamp Crayfish, Procambarus clarkii, Grown on Two Different Culture Environments

et al. 2021

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The structure and function of intestinal microorganisms are closely related to host metabolism, development, physiology, and health. The red swamp crayfish, Procambarus clarkii, is an important farmed aquatic species in China, which is grown in aquaculture ponds and rice paddy fields. Since these are two distinct cultivation environments with important differences in nutrient input and ecological community composition, we hypothesized that they may have different effects on the gut microbiota of the crayfish. Here, we sought to examine this hypothesis. To that aim, metagenomics analyses were applied to unveil the taxonomic composition and functional diversity of the microbiota in the intestines of red swamp crayfish grown in aquaculture ponds and rice-crayfish cultivation environments. The results showed that Firmicutes and Proteobacteria were the two most abundant microbial components. In addition, the relative abundance of bacterial and archaeal communities, but not that of fungal and viral communities, significantly differed between the two environments. The abundance of genes involved in pathways related to genetic information processing and human diseases was lower in the guts of red swamp crayfish grown in rice-crayfish cultivation environments. In particular, the abundance of two gene sets, K13730 and K08303, which are related to epithelial cell invasion by Listeria monocytogenes and Helicobacter pylori, respectively, decreased in this culture environment. In addition, the samples from rice-crayfish cultivation environments tended to have lower relative abundance of glycosyltransferases (GTs), which were the most abundant carbohydrate-active enzymes in the samples from both groups, higher abundance of glycoside hydrolases, and lower abundance of GT2.

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“…The red swamp crayfish Procambarus clarkii is a highly adaptable and fecund freshwater crayfish species becoming an important aquatic food sector among fishery trading commodities in China (Du et al 2017;Shen et al 2014). The production exceeded 1 million tons with 37 billion USD output in 2017, accounting for 80% of global production according to FAO statistics (FAOSTAT, http://www.fao.org/faost at/en/) and Crayfish Industry Report 2018 (http://www.moa.gov.cn/).…”

Section: Introductionmentioning

confidence: 99%

Gut microbiota of red swamp crayfish Procambarus clarkii in integrated crayfish-rice cultivation model

Zhang

et al. 2020

AMB Expr

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Increasing evidences suggest that intestinal microbiota balance closely correlated with host's health status could affected by external environment. Integrated crayfish-rice cultivation model is a highly efficient artificial ecosystem widely practiced in subtropical China. Less information is available to estimate the influence response to the micro-ecology of crayfish intestine and so as to influence the biological processes. Thus, 16S rRNA high-throughput sequencing approach was employed to investigate the composition diversity and functions of bacterial community in the intestines of Procambarus clarkii farmed within this model. Results exhibited the highly diversity of microflora with dominant phyla Actinobacteria, Proteobacteria, Tenericutes, Firmicutes and Bacteroidetes. The genera of Candidatus Bacilloplasma and Ornithinibacter were presented as predominant population much exceeds in richness comparing to that of other genus. Despite the highly diversity in the bacterial community, the predicted functions indicated relative consistent in biological processing pathway. Collectively, significant richness of genes was observed involved in amino acid and carbohydrate metabolism and membrane transport processing. This study would contribute to the understanding of the impact of growth conditions on host-microbiota relation especially in aquatic animals.

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Identification and characterization of lymph organ microRNAs in red swamp crayfish, Procambarus clarkii infected with white spot syndrome virus

Cited by 14 publications

References 30 publications

Acute Hepatopancreatic Necrosis Disease (AHPND) related microRNAs in Litopenaeus vannamei infected with AHPND-causing strain of Vibrio parahemolyticus

Acute Hepatopancreatic Necrosis Disease (AHPND) related microRNAs in Litopenaeus vannamei infected with AHPND-causing strain of Vibrio parahemolyticus

Metagenomics Analysis Reveals Compositional and Functional Differences in the Gut Microbiota of Red Swamp Crayfish, Procambarus clarkii, Grown on Two Different Culture Environments

Gut microbiota of red swamp crayfish Procambarus clarkii in integrated crayfish-rice cultivation model

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