Identification and characterization of immune-related microRNAs in blunt snout bream, Megalobrama amblycephala

Jiang, Yuhong; Tang, Leilei; Zhang, Fuyun; Jiang, Hongyang; Liu, Xiaowen; Yang, Liying; Zhang, Lei; Mao, Jingrong; Ji, Yan

doi:10.1016/j.fsi.2015.12.013

Cited by 35 publications

(8 citation statements)

References 71 publications

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“…For example, Zhang et al identified DEMs, including miR-30c and miR-145 in M. amblycephala that responded to high fat diet throughput sequencing analysis [6]. Among the identified known DEMs, miRNAs including mam-miR-192, mam-miR-128 and mam-miR-100-5p showed a relatively higher number of sequencing reads in NSD intestines, HSD intestines, NSD livers, and HSD liver libraries (Supplementary Table S4), and they had been identified to be associated with immune function and basic physical development [25,26,27,28]. In this present study, we demonstrated that mam-miR-205-5p was significantly upregulated in HSD intestines in comparison with NSD intestines (Supplementary Table S6).…”

Section: Discussionmentioning

confidence: 99%

Identification of Differentially Expressed Micrornas Associate with Glucose Metabolism in Different Organs of Blunt Snout Bream (Megalobrama amblycephala)

Miao

Lin

Pan

et al. 2017

IJMS

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Blunt snout bream (Megalobrama amblycephala) is a widely favored herbivorous fish species and is a frequentlyused fish model for studying the metabolism physiology. This study aimed to provide a comprehensive illustration of the mechanisms of a high-starch diet (HSD) induced lipid metabolic disorder by identifying microRNAs (miRNAs) controlled pathways in glucose and lipid metabolism in fish using high-throughput sequencing technologies. Small RNA libraries derived from intestines, livers, and brains of HSD and normal-starch diet (NSD) treated M. amblycephala were sequenced and 79, 124 and 77 differentially expressed miRNAs (DEMs) in intestines, livers, and brains of HSD treated fish were identified, respectively. Bioinformatics analyses showed that these DEMs targeted hundreds of predicted genes were enriched into metabolic pathways and biosynthetic processes, including peroxisome proliferator-activated receptor (PPAR), glycolysis/gluconeogenesis, and insulin signaling pathway. These analyses confirmed that miRNAs play crucial roles in glucose and lipid metabolism related to high wheat starch treatment. These results provide information on further investigation of a DEM-related mechanism dysregulated by a high carbohydrate diet.

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Section: Discussionmentioning

confidence: 99%

Identification of Differentially Expressed Micrornas Associate with Glucose Metabolism in Different Organs of Blunt Snout Bream (Megalobrama amblycephala)

Miao

Lin

Pan

et al. 2017

IJMS

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“…The small RNA transcriptome of A. hydrophila -infected M. amblycephala was analyzed in the liver because the organ plays important roles in immune responses in both mammals and fish, particularly in fish responses to A. hydrophila infection [26,27,28,29,30]. Previous studies in our lab indicated that some immune-related genes are activated at an early stage of A. hydrophila infection in M. amblycephala [31,32,33,34,35].…”

Section: Resultsmentioning

confidence: 99%

“…Studies concerning the response of miRNAs to bacterial infections have confirmed miRNAs as key players in the host innate and adaptive immune responses [23], suggesting that identification of immune-related miRNAs might facilitate the understanding of their pathological functions and the disease defense mechanisms in M. amblycephala . To date, miRNAs have been found to be involved in growth, diet-induced hepatic steatosis, intermuscular bone development and lipopolysaccharide (LPS) stimulation in M. amblycephala [8,24,25,26]. However, the involvement of M. amblycephala miRNAs in A. hydrophila infection has not been elucidated.…”

Section: Introductionmentioning

confidence: 99%

Identification and Characterization of MicroRNAs in the Liver of Blunt Snout Bream (Megalobrama amblycephala) Infected by Aeromonas hydrophila

Cui

Wei

et al. 2016

IJMS

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MicroRNAs (miRNAs) are small RNA molecules that play key roles in regulation of various biological processes. In order to better understand the biological significance of miRNAs in the context of Aeromonas hydrophila infection in Megalobrama amblycephala, small RNA libraries obtained from fish liver at 0 (non-infection), 4, and 24 h post infection (poi) were sequenced using Illumina deep sequencing technology. A total of 11,244,207, 9,212,958, and 7,939,157 clean reads were obtained from these three RNA libraries, respectively. Bioinformatics analysis identified 171 conserved miRNAs and 62 putative novel miRNAs. The existence of ten randomly selected novel miRNAs was validated by RT-PCR. Pairwise comparison suggested that 61 and 44 miRNAs were differentially expressed at 4 and 24 h poi, respectively. Furthermore, the expression profiles of nine randomly selected miRNAs were validated by qRT-PCR. MicroRNA target prediction, gene ontology (GO) annotation, and Kyoto Encylopedia of Genes and Genomes (KEGG) analysis indicated that a variety of biological pathways could be affected by A. hydrophila infection. Additionally, transferrin (TF) and transferrin receptor (TFR) genes were confirmed to be direct targets of miR-375. These results will expand our knowledge of the role of miRNAs in the immune response of M. amblycephala to A. hydrophila infection, and facilitate the development of effective strategies against A. hydrophila infection in M. amblycephala.

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“…Among the 6 miRNAs identified by deep sequencing as being responsive only to ASAL, miR-727a-3p was qPCR confirmed as being significantly down-regulated compared with PBS control in fish fed the control diet. Similarly, the expression of miR-727-3p was reduced in the LPS-stimulated blunt snout bream (Megalobrama amblycephala) [67]. It should be noted that miR-727 is likely a teleost-specific miRNA [68], and the role of miR-727a-3p in the antibacterial immune response in fish is yet to be determined.…”

Section: Mirnas Only Associated With Antibacterial Immune Response Inmentioning

confidence: 99%

Dietary Immunostimulant CpG Modulates MicroRNA Biomarkers Associated with Immune Responses in Atlantic Salmon (Salmo salar)

Xue

Woldemariam

Caballero‐Solares

et al. 2019

Cells

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MicroRNAs (miRNAs) are key regulators in fish immune responses. However, no study has previously characterized the impact of polyriboinosinic polyribocytidylic acid (pIC) and formalin-killed typical Aeromonas salmonicida (ASAL) on miRNA expression in Atlantic salmon fed a commercial diet with and without immunostimulant CpG. To this end, first, we performed small RNA deep sequencing and qPCR analyses to identify and confirm pIC- and/or ASAL-responsive miRNAs in the head kidney of salmon fed a control diet. DESeq2 analyses identified 12 and 18 miRNAs differentially expressed in pIC and ASAL groups, respectively, compared to the controls. Fifteen of these miRNAs were studied by qPCR; nine remained significant by qPCR. Five miRNAs (miR-27d-1-2-5p, miR-29b-2-5p, miR-146a-5p, miR-146a-1-2-3p, miR-221-5p) were shown by qPCR to be significantly induced by both pIC and ASAL. Second, the effect of CpG-containing functional feed on miRNA expression was investigated by qPCR. In pre-injection samples, 6 of 15 miRNAs (e.g., miR-181a-5-3p, miR-462a-3p, miR-722-3p) had significantly lower expression in fish fed CpG diet than control diet. In contrast, several miRNAs (e.g., miR-146a-1-2-3p, miR-192a-5p, miR-194a-5p) in the PBS- and ASAL-injected groups had significantly higher expression in CpG-fed fish. Multivariate statistical analyses confirmed that the CpG diet had a greater impact on miRNA expression in ASAL-injected compared with pIC-injected fish. This study identified immune-relevant miRNA biomarkers that will be valuable in the development of diets to combat infectious diseases of salmon.

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Identification and characterization of immune-related microRNAs in blunt snout bream, Megalobrama amblycephala

Cited by 35 publications

References 71 publications

Identification of Differentially Expressed Micrornas Associate with Glucose Metabolism in Different Organs of Blunt Snout Bream (Megalobrama amblycephala)

Identification of Differentially Expressed Micrornas Associate with Glucose Metabolism in Different Organs of Blunt Snout Bream (Megalobrama amblycephala)

Identification and Characterization of MicroRNAs in the Liver of Blunt Snout Bream (Megalobrama amblycephala) Infected by Aeromonas hydrophila

Dietary Immunostimulant CpG Modulates MicroRNA Biomarkers Associated with Immune Responses in Atlantic Salmon (Salmo salar)

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