Identification and Characterization of<i>Fringilla coelebs</i>Papillomavirus 1 (FcPV1) in Free-living and Captive Birds in Italy

Prosperi, Alice; Chiari, Mario; Zanoni, Mariagrazia; Gallina, Laura; Casa, Giovanni Della; Scagliarini, Alessandra; Llombart‐Bosch, Antonio

doi:10.7589/2015-09-254

Cited by 8 publications

(11 citation statements)

References 12 publications

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“…Domestic mammals, such as cattle and dogs, are particularly well represented with 23 Bovine PV types and 20 Canine PV types, respectively (PaVE; available at https://pave.niaid.nih.gov/ 11 ). Only eight genotypes have been found in birds 13 – 15 —from diverse avian orders—three from reptiles 16 and one from a fish 17 . Recently, a new PV genotype was described from a duck microbiome collected in Bhopal, India.…”

Section: Introductionmentioning

confidence: 99%

Molecular identification of papillomavirus in ducks

Williams

Tolf

Waldenström

2018

Sci Rep

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Papillomaviruses infect many vertebrates, including birds. Persistent infections by some strains can cause malignant proliferation of cells (i.e. cancer), though more typically infections cause benign tumours, or may be completely subclinical. Sometimes extensive, persistent tumours are recorded–notably in chaffinches and humans. In 2016, a novel papillomavirus genotype was characterized from a duck faecal microbiome, in Bhopal, India; the sixth papillomavirus genotype from birds. Prompted by this finding, we screened 160 cloacal swabs and 968 faecal samples collected from 299 ducks sampled at Ottenby Bird Observatory, Sweden in 2015, using a newly designed real-time PCR. Twenty one samples (1.9%) from six individuals (2%) were positive. Eighteen sequences were identical to the published genotype, duck papillomavirus 1. One additional novel genotype was recovered from three samples. Both genotypes were recovered from a wild strain domestic mallard that was infected for more than 60 days with each genotype. All positive individuals were adult (P = 0.004). Significantly more positive samples were detected from swabs than faecal samples (P < 0.0001). Sample type data suggests transmission may be via direct contact, and only infrequently, via the oral-faecal route. Infection in only adult birds supports the hypothesis that this virus is sexually transmitted, though more work is required to verify this.

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Section: Introductionmentioning

confidence: 99%

Molecular identification of papillomavirus in ducks

Williams

Tolf

Waldenström

2018

Sci Rep

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“…FcPV1 sequences (including one full genome of ca. 7700 bp) have been previously reported from chaffinches in Madeira, the Netherlands, Sweden, Spain and Italy 6 , 11 , 13 , 31 , 44 . All the sequences recovered in the current study, indeed all modern sequences, are identical, belonging to one dominant haplotype.…”

Section: Discussionmentioning

confidence: 66%

“…FcPV1 and Serinus canaria papillomavirus 1, the papillomavirus most similar to FcPV1, share less than 68% nucleotide identity. In contrast, known FcPV1 sequences differ in this region of the genome by a maximum of 1 nucleotide 6 , 31 . In the current study, we document the first papillomavirus sequences for bullfinch, greenfinch and goldfinch.…”

Section: Discussionmentioning

confidence: 93%

“…Prosperi et al . 6 ), offers an alternative for confirmation of viral infection; however, such studies require optimal tissue preservation which was not possible in the current study due to the nature of sample acquisition.…”

Section: Discussionmentioning

confidence: 97%

“…Chaffinches with proliferative leg skin lesions associated with papillomavirus infection have been described from multiple countries in western, northern and central Europe, including the Czech Republic, Germany, Great Britain, Italy, Sweden and the Netherlands 6 , 10 – 14 . Papillomavirus from similar leg skin lesions has been reported less commonly in wild brambling ( Fringilla montifringilla ), wild bullfinch ( Pyrrhula pyrrhula ) and captive greenfinch ( Chloris chloris ) 2 , 6 , 15 . The macroscopic appearance of these proliferative leg skin lesions has been described generally as (squamous) papillomas and wart-like growths and more specifically as nodular or hyperplastic lesions with deeply fissured papillary growths (i.e.…”

Section: Introductionmentioning

confidence: 99%

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Spatio-temporal dynamics and aetiology of proliferative leg skin lesions in wild British finches

Lawson

Robinson

Fernández³

et al. 2018

Sci Rep

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Proliferative leg skin lesions have been described in wild finches in Europe although there have been no large-scale studies of their aetiology or epizootiology to date. Firstly, disease surveillance, utilising public reporting of observations of live wild finches was conducted in Great Britain (GB) and showed proliferative leg skin lesions in chaffinches (Fringilla coelebs) to be widespread. Seasonal variation was observed, with a peak during the winter months. Secondly, pathological investigations were performed on a sample of 39 chaffinches, four bullfinches (Pyrrhula pyrrhula), one greenfinch (Chloris chloris) and one goldfinch (Carduelis carduelis) with proliferative leg skin lesions and detected Cnemidocoptes sp. mites in 91% (41/45) of affected finches and from all species examined. Fringilla coelebs papillomavirus (FcPV1) PCR was positive in 74% (23/31) of birds tested: a 394 base pair sequence was derived from 20 of these birds, from all examined species, with 100% identity to reference genomes. Both mites and FcPV1 DNA were detected in 71% (20/28) of birds tested for both pathogens. Histopathological examination of lesions did not discriminate the relative importance of mite or FcPV1 infection as their cause. Development of techniques to localise FcPV1 within lesions is required to elucidate the pathological significance of FcPV1 DNA detection.

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