Identification and Characterization of Glycosyltransferase 43 (GT43) Gene Family in Sorghum bicolor

Abstract

Sorghum (Sorghum bicolor) is an essential bioenergy crop. Cellulosic and non-cellulosic polysaccharides, which can both be transformed into biofuels, make up a majority of their biomass. Many glycosyltransferases (GT) families, including GT43, are involved in the biosynthesis of xylan in the primary and secondary cells of plants. In this study, we performed identification, phylogenetic tree, physiochemical properties, subcellular localization, protein-protein interaction network, detection of motifs analysis, gene structure, secondary and three-dimensional (3D) model construction, functional domain, gene duplication, cis-acting elements, sequences logos, multiple sequence alignment and gene expression profiles based on RNA-sequence analyses in GT47 gene family. As a result, we identified eleven members of the GT43 gene family. The eleven GT43 genes in sorghum that we were able to identify and explain here. The phylogenetic tree of the GT43 genes family showed that all GT43 genes have evolutionary relationships with each other in sorghum. Gene structure, motifs, sequence logos, and multiple sequence alignment analysis showed that all members of the GT47 protein were highly conserved. Subcellular localization showed all members of GT43 proteins were localized in the different compartments of sorghum. The secondary structure of GT43 genes was made up of a different percentage of α-helix, random coils, β-turn, and extended strands and the tertiary structure model showed that all GT43 proteins had similar 3D structures. Current results indicated that GT43 members of the GT43 gene family (Sobic.010G238800,Sobic.003G254700 Sobic.001G409100) were highly expressed in internodes of sorghum plant based on RNA-Sequence. These results indicated the extensive and dependent relationship of GT43 genes involved in sorghum (S. bicolor). This study will also help formulate accurate and precise laboratory experiments for future use.