Identification and Characterization of Forced Degradation Products for Dofetilide using Rapid and Sensitive UPLC-MS/MS Method and HRMS Studies

Abstract

A simple, rapid and efficient method has been developed and validated using ultra UPLC combined with Q-ToF MS system for recognition and characterization of forced degradation products obtained from dofetilide degradation studies. The dofetilide drug is an antiarrhythmic and belongs to Class III and it was treated with various stress conditions like acidic, basic, oxidative, photolytic and thermal conditions as per ICH guidelines. The main drug shows extensive degradation towards oxidative degradation conditions and single degradation product was identified through chromatogram. The chromatographic separation among main and its impurities were attained through 2.1 × 150, 1.8 μm column from gradient elution using UPLC and its detection at wavelength 230 nm. The validation was performed for the developed method using various parameters like specificity, linearity and robustness studies. Waters Synapt G2 Q TOF system was used and performed MSn studies to establish mass spectral fragmentation pathway for drug and its degradation products and determined accurate masses study. The efficiency of this method was helpful to identify and characterize the drug and degradation products using LC/MSn techniques.

Sensitive and validated UPLC-MS/MS method for the identification and characterization of forced degradation products for felodipine

“…The parameters set for MS studies were as follows: cone voltage: 3.0 v, capillary voltage: 3.2 v, source temperature: 100 8C, desolvation temperature: 250 8C and gas flow: 800 L h À1 . Masslynx 4.1 software was used for data acquisition [15][16][17][18][19].…”

“…Moreover, UPLC is a common laboratory technique that decreases the cost of designing and validating a method while increasing the efficiency of analysis. The speed of separation and efficiency improve with UPLC, resulting in the rapid development of techniques [13][14][15][16][17][18][19]. The goal of ultra-performance liquid chromatography (UPLC) drug analysis is to authenticate a drug's identity and provide quantitative data, as well as to follow the progress of a disease's treatment [8].…”