volume 278, issue 31, P28892-28900 2003
DOI: 10.1074/jbc.m302890200
View full text

Abstract: To identify deneddylases, proteases with specificity for hydrolysis of Nedd8 derivatives, a facile method was developed for the synthesis of Nedd8 amidomethylcoumarin (a substrate) and Nedd8 vinyl sulfone (an inhibitor). Deneddylase activity is necessary to reverse the conjugation of Nedd8 to cullin, a modification that regulates at least some ubiquitin ligases. The reaction of Nedd8 vinyl sulfone with L-M(TK ؊ ) mouse fibroblast lysates identified two deneddylases. The deubiquitinating enzyme UCH-L3 is label…

Expand abstract