Identification and characterization of Corynebacterium lactis isolated from Amblyomma testudinarium of Sus scrofa in Malaysia

Lim, Fang-Shiang; Loong, Shih Keng; Khoo, Jing Jing; Tan, Kian Lam; Zainal, Nurhafiza; Abdullah, MuhammadFirdaus; Khor, Chee‐Sieng; AbuBakar, Sazaly

doi:10.11158/saa.23.9.10

Cited by 10 publications

(8 citation statements)

References 26 publications

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“…We employed a non-selective bacterial culture method in this study, previously shown to enable isolation of rare and pathogenic bacteria species from tick specimens such as Corynebacterium lactis (Lim et al, 2018) and P. lautus (Loong et al, 2018a). Even though no bacterial growth was observed for some tick specimens, we suggest that it was not due to the limitation of the agar culture media but the method employed for bacterial isolation (Stewart, 2012).…”

Section: Discussionmentioning

confidence: 95%

Culturable pathogenic bacteria in ticks parasitizing farm animals and rodents in Malaysia

et al. 2020

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Section: Discussionmentioning

confidence: 95%

Culturable pathogenic bacteria in ticks parasitizing farm animals and rodents in Malaysia

et al. 2020

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“…Visual inspection of the bacterial isolate after incubation in a 96-well plate at 37°C for 48 h [10] noted that the C. lipophiloflavum isolate did not produce biofilm. Morphological characteristics of the bacterium unveiled by transmission electron microscopy [11] displayed structures similar to C. phoceense [12] and C. lactis [13] [Figure . 2]. The bacterium showed characteristic clubshaped cell and the presence of an external lipid layer.…”

Section: Resultsmentioning

confidence: 99%

“…Although the C. lipophiloflavum isolate did not produce biofilm, it may switch from the dormant state to biofilm producer [17] on leaving the normal flora, as a result of selection and environmental pressures, further suggesting the undesirable influence of corynebacteria in dairy contamination. While C. lactis may seem harmless when it was first isolated from the cow's milk, recent studies have shown that it was also found in ticks and can cause infection in companion animals [13]. Hence, treating C. lipophiloflavum as a harmless normal flora of the cow could have disastrous aftermath to the entire dairy supply chain.…”

Section: Resultsmentioning

confidence: 99%

Microbiological analysis of raw milk unveiled the presence of a dairy contaminant, Corynebacterium lipophiloflavum

2019

Jabb

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Dairy farming occupied a distinct position in agriculture since milk can be harvested every day, providing a regular source of income to the farmers. Development of the Malaysian dairy farming industry was marred by poor farm hygiene practices, leading to the proliferation of dairy-spoilage bacteria, affecting milk quality. In this study, we report the isolation and characterization of a rare Corynebacterium species from raw milk after the implementation of improved farm hygiene practices. All milking equipment, farm worker's hands and the cow's udders and teats were washed with detergent and wiped dry with clean towels before milk sample collection. Collected foremilk samples from mastitis-free cows were inoculated onto Petrifilm™ and cultured colonies were plated onto nutrient agar. Biochemical and molecular tests were performed for the identification of peculiar bacterial isolates. A unique yellow-pigmented bacteria isolate was recovered from the milk of a healthy cow after the adoption of improved farm hygiene practices. Phenotypic and genotypic characterization confirmed the milk isolate as Corynebacterium lipophiloflavum. This is the first description of C. lipophiloflavum in cow's milk and could possibly imply the influence of bovine flora in dairy contamination. The findings highlight the increasing spectrum of Corynebacterium species with potential adverse impact to the dairy industry. It is recommended to screen for C. lipophiloflavum in all milk processing facility to ensure that milk is safe for consumption and its products prepared to the highest quality and safety standards.

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“…C. lactis and other Corynebacterium species are frequently found in raw milk (13, 30) and some isolates of Corynebacterium bovis and C. amycolatum are causative agents of mastitis in dairy cows (53, 54). C. lactis was eventually linked to infections in companion animals, but is not considered a pathogen so far and the determinants of infection remain unclear (55, 56). Interestingly, a putative BGC similar to corynaridin exists in the genome of an isolate of C. striatum from an intensive care unit (49).…”

Section: Discussionmentioning

confidence: 99%

Identification, Characterization and Mode of Action of Corynaridin, a Novel Linaridin from Corynebacterium lactis

Pashou

Reich

Weixler

et al. 2022

Preprint

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Genome analysis of Corynebacterium lactis revealed a bacteriocin gene cluster encoding a putative bacteriocin of the linaridin family of ribosomally synthesized and posttranslationally modified peptides (RIPPs). The locus harbors typical linaridin modification enzymes but lacks genes for a decarboxylase and methyltransferase, which is unusual for type B linaridins. Supernatants of Corynebacterium lactis RW3-42 showed antimicrobial activity against Corynebacterium glutamicum. Deletion of the precursor gene crdA clearly linked the antimicrobial activity of the producer strain to the identified gene cluster. Following purification, we observed potent activity of the peptide against Actinobacteria, mainly other members of the genus Corynebacterium including the pathogenic species Corynebacterium striatum and Corynebacterium amycolatum. Also, low activity against some Firmicutes was observed, but no activity against Gram-negative species. The peptide is resilient towards heat but sensitive to proteolytic degradation by trypsin and proteinase K. Notably, time-kill kinetics and experiments using live biosensors to monitor membrane integrity suggest bactericidal activity that does not involve formation of pores in the cytoplasmic membrane. As Corynebacterium species are ubiquitous in nature and include important commensal and pathogens of mammalian organisms, secretion of bacteriocins by species of this genus could be a hitherto neglected trait with high relevance for intra- and interspecies competition and infection.ImportanceBacteriocins are antimicrobial peptides produced by bacteria to fend off competitors in ecological niches and are considered to be important factors influencing the composition of microbial communities. However, bacteriocin production by bacteria of the genus Corynebacterium has been a hitherto neglected trait, although its species are ubiquitous in nature and make up large parts of the microbiome of humans and animals. In this study, we describe and characterize a novel linaridin-family bacteriocin from Corynebacterium lactis and show its narrow spectrum activity, mainly against other actinobacteria. Moreover, we were able to extend the limited knowledge on linaridin bioactivity in general and for the first time describe the mode of action of such a bacteriocin. Interestingly, the peptide, which was named corynaridin, appears bactericidal, but without formation of pores in the bacterial membrane.

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Identification and characterization of Corynebacterium lactis isolated from Amblyomma testudinarium of Sus scrofa in Malaysia

Cited by 10 publications

References 26 publications

Culturable pathogenic bacteria in ticks parasitizing farm animals and rodents in Malaysia

Culturable pathogenic bacteria in ticks parasitizing farm animals and rodents in Malaysia

Microbiological analysis of raw milk unveiled the presence of a dairy contaminant, Corynebacterium lipophiloflavum

Identification, Characterization and Mode of Action of Corynaridin, a Novel Linaridin from Corynebacterium lactis

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